Job Description P11553 Confidence 100.00% Date Thu Jan 5 11:32:45 GMT 2012 Rank 3 Aligned Residues 462 % Identity 22% Template c3flcX_ PDB info PDB header:transferase Chain: X: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of the his-tagged h232r mutant of glycerol kinase2 from enterococcus casseliflavus with glycerol Resolution 1.85 Å

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		12	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.			.	80	.	.	.	.	.	.	.	.	.
Predicted Secondary structure																																																																					.	.
Query SS confidence																																																																					.	.
Query Sequence		K	Q	E	V	I	L	V	L	D	C	G	A	T	N	V	R	A	I	A	V	N	R	Q	G	K	I	V	A	R	A	S	T	P	N	A	S	D	I	A	M	E	N	N	T	W	H	Q	W	S	L	D	A	I	L	Q	R	F	A	D	C	C	R	Q	I	N	S	E	.	.	L	T	E	C	H	I	R	G	I	A	V
Query Conservation																																																																					.	.
Alig confidence																																					.	.																															.	.
Template Conservation																																					.	.
Template Sequence		E	K	N	Y	V	M	A	I	D	Q	G	T	T	S	S	R	A	I	I	F	D	R	N	G	K	K	I	G	S	S	Q	K	E	F	P	.	.	Q	Y	F	P	K	S	G	W	V	E	H	N	A	N	E	I	W	N	S	V	Q	S	V	I	A	G	A	F	I	E	S	G	I	R	P	E	A	I	A	G	I	G	I
Template Known Secondary structure													S	S									T	T	S												.	.		B		S	S	T	T																												G	G	G
Template Predicted Secondary structure																																					.	.
Template SS confidence
		3	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.			.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80
		90	.	.	.	.	.	.	.	.	.	100	.		.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.
Predicted Secondary structure														.																																																																		.	.
Query SS confidence														.																																																																		.	.
Query Sequence		T	T	F	G	V	D	G	A	L	V	D	K	.	Q	G	N	L	L	Y	P	I	I	S	W	K	C	P	R	T	A	A	V	M	D	N	I	E	R	L	I	S	A	Q	R	L	Q	A	I	S	G	V	G	A	F	S	F	N	T	L	Y	K	L	V	W	L	K	E	N	H	P	Q	L	L	E	R	A	H	A	.	.
Query Conservation														.																																																																		.	.
Alig confidence														.																																																																		.	.
Template Conservation
Template Sequence		T	N	Q	R	E	T	T	V	V	W	D	K	T	T	G	Q	P	I	A	N	A	I	V	W	Q	S	R	Q	S	S	P	I	A	D	Q	L	K	V	D	G	H	T	E	M	I	H	E	K	T	G	L	V	I	D	A	Y	F	S	A	T	K	V	R	W	L	L	D	N	I	E	G	A	Q	E	K	A	D	N	G	E
Template Known Secondary structure					S	S							T	T	T			B	S	S					T	T			T	T										T	T										S					T	T	S												S	T	T							T	T
Template Predicted Secondary structure
Template SS confidence
		81	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160
				167	.	.	170	.	.	.	.	.	.	.	.	.	180	.			.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.
Predicted Secondary structure		.	.																.	.
Query SS confidence		.	.																.	.
Query Sequence		.	.	W	L	F	I	S	S	L	I	N	H	R	L	T	G	E	.	.	F	T	T	D	I	T	M	A	G	T	S	Q	M	L	D	I	Q	Q	R	D	F	S	P	Q	I	L	Q	A	T	G	I	P	R	R	L	F	P	R	L	V	E	A	G	E	Q	I	G	T	L	Q	N	S	A	A	A	M	L	G	L	P	V
Query Conservation		.	.																.	.
Alig confidence		.	.																.	.																																																					.	.	.	.
Template Conservation																																																																									.	.	.	.
Template Sequence		L	L	F	G	T	I	D	S	W	L	V	W	K	L	T	D	G	Q	V	H	V	T	D	Y	S	N	A	S	R	T	M	L	Y	N	I	H	K	L	E	W	D	Q	E	I	L	D	L	L	N	I	P	S	S	M	L	P	E	V	K	S	N	S	E	V	Y	G	H	T	R	S	Y	.	.	.	.	R	F	Y	G	S
Template Known Secondary structure																T	T	T	S											T	T	T	S			T	T	T	T										T	T			G	G	G							S									G	G	.	.	.	.	G	G	T	T
Template Predicted Secondary structure																																																																									.	.	.	.
Template SS confidence
		161	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.					.	.	.	.	.
		243	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.		.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.
Predicted Secondary structure																						.
Query SS confidence																						.
Query Sequence		G	I	P	V	I	S	A	G	H	D	T	Q	F	A	L	F	G	A	G	A	.	E	Q	N	E	P	V	L	S	S	G	T	W	E	I	L	M	V	R	S	A	Q	V	D	T	S	L	L	S	Q	Y	A	G	S	T	C	E	L	D	S	Q	A	G	L	Y	N	P	G	M	Q	W	L	A	S	G	V	L	E	W	V
Query Conservation																						.
Alig confidence																						.
Template Conservation
Template Sequence		E	V	P	I	A	G	M	A	G	D	Q	Q	A	A	L	F	G	Q	M	A	F	E	K	G	M	I	K	N	T	Y	G	T	G	A	F	I	V	M	N	T	G	E	E	P	L	S	D	N	D	L	L	T	T	I	G	Y	G	I	N	G	K	V	Y	Y	A	L	E	G	S	I	F	V	A	G	S	A	I	Q	W	L
Template Known Secondary structure																			T	T			S	T	T							S	S	S									T						T	T	S									T	T											S		S
Template Predicted Secondary structure
Template SS confidence
		237	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.
		322	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.									.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.
Predicted Secondary structure																																											.	.	.	.	.	.	.	.
Query SS confidence																																											.	.	.	.	.	.	.	.
Query Sequence		R	K	L	F	W	T	A	E	T	P	W	Q	M	L	I	E	E	A	R	L	I	A	P	G	A	D	G	V	K	M	Q	C	D	L	L	S	C	Q	N	A	G	.	.	.	.	.	.	.	.	W	Q	G	V	T	L	N	T	T	R	G	H	F	Y	R	A	A	L	E	G	L	T	A	Q	L	Q	R	N	L	Q	M
Query Conservation																																											.	.	.	.	.	.	.	.
Alig confidence										.	.																																.	.	.	.	.	.	.	.
Template Conservation										.	.
Template Sequence		R	D	G	L	R	M	I	E	.	.	T	S	P	Q	S	E	E	L	A	A	K	A	K	G	D	N	E	V	Y	V	V	P	A	F	T	G	L	G	A	P	Y	W	D	S	E	A	R	G	A	V	F	G	L	T	R	G	T	T	K	E	D	F	V	R	A	T	L	Q	A	V	A	Y	Q	S	K	D	V	I	D	T
Template Known Secondary structure				T	S			S	S	.	.	S	T	T											S		S								T	T		B		T	T	T		B	T	T										T	T
Template Predicted Secondary structure										.	.
Template SS confidence
		318	.	320	.	.	.	.	.			.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.
		394	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		L	E	K	I	G	H	F	K	A	S	E	L	L	L	V	G	G	G	S	R	N	T	L	W	N	Q	I	K	A	N	M	L	D	I	P	V	K	V	L	D	D	A	E	T	T	V	A	G	A	A	L	F	G	W	Y	G	V	G	E	F	N	S	P	E	E	A	R	A	Q	I	H	Y	Q	Y	R	Y	F	Y	P	Q
Query Conservation
Alig confidence																																																																				.
Template Conservation																																																																				.
Template Sequence		M	K	K	D	S	G	I	D	I	P	L	L	K	V	D	G	G	A	A	K	N	D	L	L	M	Q	F	Q	A	D	I	L	D	I	D	V	Q	R	A	A	N	L	E	T	T	A	L	G	A	A	Y	L	A	G	L	A	V	G	F	W	K	D	L	D	E	L	.	K	S	M	A	E	E	G	Q	M	F	T	P	E
Template Known Secondary structure																	S	G	G	G	G													T	S						S	S	S															T	T	S	S							.			T
Template Predicted Secondary structure																																																																				.
Template SS confidence
		396	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.		.	.	.	.	.	.	.	.	470	.	.	.	.
		474	.	.	.	.	.	480	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		T	E	P	E	F	I	E	E	V
Query Conservation
Alig confidence
Template Conservation
Template Sequence		M	P	A	E	E	R	D	N	L
Template Known Secondary structure		S
Template Predicted Secondary structure
Template SS confidence
		475	.	.	.	.	480	.	.	.