Job Description P11553 Confidence 100.00% Date Thu Jan 5 11:32:45 GMT 2012 Rank 15 Aligned Residues 444 % Identity 20% Template c3ezwD_ PDB info PDB header:transferase Chain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices Resolution 2.00 Å

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		12	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.			80	.	.	.	.	.	.	.	.	.
Predicted Secondary structure																																																																						.	.
Query SS confidence																																																																						.	.
Query Sequence		K	Q	E	V	I	L	V	L	D	C	G	A	T	N	V	R	A	I	A	V	N	R	Q	G	K	I	V	A	R	A	S	T	P	N	A	S	D	I	A	M	E	N	N	T	W	H	Q	W	S	L	D	A	I	L	Q	R	F	A	D	C	C	R	Q	I	N	S	E	L	.	.	T	E	C	H	I	R	G	I	A	V
Query Conservation																																																																						.	.
Alig confidence																																					.	.																																.	.
Template Conservation																																					.	.
Template Sequence		E	K	K	Y	I	V	A	L	D	Q	G	T	T	S	S	R	A	V	V	M	D	H	D	A	N	I	I	S	V	S	Q	R	E	F	E	.	.	Q	I	Y	P	K	P	G	W	V	E	H	D	P	M	E	I	W	A	T	Q	S	S	T	L	V	E	V	L	A	K	A	D	I	S	S	D	Q	I	A	A	I	G	I
Template Known Secondary structure													S	S									T	T													.	.		B			S	T	T																								T	T			G	G	G
Template Predicted Secondary structure																																					.	.
Template SS confidence
		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.			.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.
		90	.	.	.	.	.	.	.	.	.	100	.		.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.
Predicted Secondary structure														.																																																																		.	.
Query SS confidence														.																																																																		.	.
Query Sequence		T	T	F	G	V	D	G	A	L	V	D	K	.	Q	G	N	L	L	Y	P	I	I	S	W	K	C	P	R	T	A	A	V	M	D	N	I	E	R	L	I	S	A	Q	R	L	Q	A	I	S	G	V	G	A	F	S	F	N	T	L	Y	K	L	V	W	L	K	E	N	H	P	Q	L	L	E	R	A	H	A	.	.
Query Conservation														.																																																																		.	.
Alig confidence														.																																																																		.	.
Template Conservation
Template Sequence		T	N	Q	R	E	T	T	I	V	W	E	K	E	T	G	K	P	I	Y	N	A	I	V	W	Q	C	R	R	T	A	E	I	C	E	H	L	K	R	D	G	L	E	D	Y	I	R	S	N	T	G	L	V	I	D	P	Y	F	S	G	T	K	V	K	W	I	L	D	H	V	E	G	S	R	E	R	A	R	R	G	E
Template Known Secondary structure					S	S		B					T	T	T			B	S	S					T	T			T	T										T	T										S			S	S	T	T	S												S	T	T							T	T	S
Template Predicted Secondary structure
Template SS confidence
		80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.
				167	.	.	170	.	.	.	.	.	.	.	.	.	180			.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.
Predicted Secondary structure		.	.															.	.
Query SS confidence		.	.															.	.
Query Sequence		.	.	W	L	F	I	S	S	L	I	N	H	R	L	T	G	.	.	E	F	T	T	D	I	T	M	A	G	T	S	Q	M	L	D	I	Q	Q	R	D	F	S	P	Q	I	L	Q	A	T	G	I	P	R	R	L	F	P	R	L	V	E	A	G	E	Q	I	G	T	L	Q	N	S	A	A	A	M	L	G	L	P	V
Query Conservation		.	.															.	.
Alig confidence		.	.															.	.																																																			.	.	.	.	.	.	.	.	.	.	.
Template Conservation																																																																						.	.	.	.	.	.	.	.	.	.	.
Template Sequence		L	L	F	G	T	V	D	T	W	L	I	W	K	M	T	Q	G	R	V	H	V	T	D	Y	T	N	A	S	R	T	M	L	F	N	I	H	T	L	D	W	D	D	K	M	L	E	V	L	D	I	P	R	E	M	L	P	E	V	R	R	S	S	E	V	Y	G	Q	T	.	.	.	.	.	.	.	.	.	.	.	N
Template Known Secondary structure																T	T	T	S										G	G	G	S	S			T	T	T	T										T	T			G	G	G							S								.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																																																																						.	.	.	.	.	.	.	.	.	.	.
Template SS confidence
		160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.												.
		243	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.		.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.		.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320
Predicted Secondary structure																						.																																.
Query SS confidence																						.																																.
Query Sequence		G	I	P	V	I	S	A	G	H	D	T	Q	F	A	L	F	G	A	G	A	.	E	Q	N	E	P	V	L	S	S	G	T	W	E	I	L	M	V	R	S	A	Q	V	D	T	S	L	L	S	Q	Y	A	.	G	S	T	C	E	L	D	S	Q	A	G	L	Y	N	P	G	M	Q	W	L	A	S	G	V	L	E	W
Query Conservation																						.																																.
Alig confidence																						.																																.
Template Conservation
Template Sequence		R	I	P	I	S	G	I	A	G	D	Q	Q	A	A	L	F	G	Q	L	C	V	K	E	G	M	A	K	N	T	Y	G	T	G	C	F	M	L	M	N	T	G	E	K	A	V	K	S	E	N	G	L	L	T	T	I	A	C	G	P	T	G	E	V	N	Y	A	L	E	G	A	V	F	M	A	G	A	S	I	Q	W
Template Known Secondary structure																			T	T			S	T	T							S	S	S								T	T	S					S	S	S									T	T	S											S		S
Template Predicted Secondary structure
Template SS confidence
		236	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.
		321	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.									.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.
Predicted Secondary structure																																												.	.	.	.	.	.	.	.
Query SS confidence																																												.	.	.	.	.	.	.	.
Query Sequence		V	R	K	L	F	W	T	A	E	T	P	W	Q	M	L	I	E	E	A	R	L	I	A	P	G	A	D	G	V	K	M	Q	C	D	L	L	S	C	Q	N	A	G	.	.	.	.	.	.	.	.	W	Q	G	V	T	L	N	T	T	R	G	H	F	Y	R	A	A	L	E	G	L	T	A	Q	L	Q	R	N	L	Q
Query Conservation																																												.	.	.	.	.	.	.	.
Alig confidence											.	.	.	.	.	.																												.	.	.	.	.	.	.	.
Template Conservation											.	.	.	.	.	.
Template Sequence		L	R	D	E	M	K	L	I	D	.	.	.	.	.	.	S	E	Y	F	A	T	K	V	Q	N	T	N	G	V	Y	V	V	P	A	F	T	G	L	G	A	P	Y	W	D	P	Y	A	R	G	A	I	F	G	L	T	R	G	V	N	A	N	H	I	I	R	A	T	L	E	S	I	A	Y	Q	T	R	D	V	L	E
Template Known Secondary structure					T	S					.	.	.	.	.	.							T	S	S	S		T	T							T	T		B		T	T	T		B	T	T										T	T
Template Predicted Secondary structure											.	.	.	.	.	.
Template SS confidence
		316	.	.	.	320	.	.	.	.							.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.
		393	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		M	L	E	K	I	G	H	F	K	A	S	E	L	L	L	V	G	G	G	S	R	N	T	L	W	N	Q	I	K	A	N	M	L	D	I	P	V	K	V	L	D	D	A	E	T	T	V	A	G	A	A	L	F	G	W	Y	G	V	G	E	F	N	S	P	E	E	A	R	A	Q	I	H	Y	Q	Y	R	Y	F	Y	P
Query Conservation
Alig confidence																																									.	.	.																									.	.	.	.
Template Conservation																																									.	.	.																									.	.	.	.
Template Sequence		A	M	Q	A	D	S	G	I	R	L	H	A	L	R	V	D	G	G	A	V	A	N	N	F	L	M	Q	F	Q	S	D	I	L	G	T	R	V	E	R	.	.	.	P	E	V	T	A	L	G	A	A	Y	L	A	G	L	A	V	G	F	W	Q	N	L	D	E	.	.	.	.	L	Q	E	I	E	R	E	F	R	P
Template Known Secondary structure												S						S	G	G	G	G													T	S					.	.	.																	T	S	S	S					.	.	.	.		T
Template Predicted Secondary structure																																									.	.	.																									.	.	.	.
Template SS confidence
		394	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.				.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.					.	.	.	460	.	.	.	.	.	.
		473	.	.	.	.	.	.	480	.
Predicted Secondary structure
Query SS confidence
Query Sequence		Q	T	E	P	E	F	I	E	E
Query Conservation
Alig confidence
Template Conservation
Template Sequence		G	I	E	T	T	E	R	N	Y
Template Known Secondary structure			S
Template Predicted Secondary structure
Template SS confidence
		473	.	.	.	.	.	.	480	.