Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	L	I	W	S	E	E	S	W	D	D	Y	L	Y	W	Q	E	T	D	K	R	I	V	K	K	I	N	E	L	I	K	D	T	R	R	T	P	F	E	G	K	G	K	P	E	P	L	K	H	N	L	S	G	F	W	S	R	R	I
Secondary structure
SS confidence
Disorder		?	?																			?																			?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.
Sequence		T	E	E	H	R	L	V	Y	A	V	T	D	D	S	L	L	I	A	A	C	R	Y	H	Y
Secondary structure
SS confidence
Disorder																							?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2a6s chain A domain 1 Region: 1 - 83 Aligned: 83 Modelled: 83 Confidence: 100.0% Identity: 100% Fold: RelE-like Superfamily: RelE-like Family: YoeB/Txe-like Phyre2 PDB 3oei chain H Region: 2 - 84 Aligned: 83 Modelled: 83 Confidence: 100.0% Identity: 46% PDB header:toxin, protein binding Chain: H: PDB Molecule:relk (toxin rv3358); PDBTitle: crystal structure of mycobacterium tuberculosis reljk (rv3357-rv3358-2 relbe3) Phyre2 PDB 1z8m chain A domain 1 Region: 1 - 83 Aligned: 83 Modelled: 83 Confidence: 99.8% Identity: 19% Fold: RelE-like Superfamily: RelE-like Family: RelE-like Phyre2 PDB 2otr chain A Region: 1 - 83 Aligned: 83 Modelled: 83 Confidence: 99.8% Identity: 18% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein hp0892; PDBTitle: solution structure of conserved hypothetical protein hp0892 from2 helicobacter pylori Phyre2 PDB 2khe chain A Region: 2 - 83 Aligned: 78 Modelled: 82 Confidence: 99.4% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:toxin-like protein; PDBTitle: solution structure of the bacterial toxin rele from thermus2 thermophilus hb8 Phyre2 PDB 3bpq chain D Region: 3 - 83 Aligned: 76 Modelled: 81 Confidence: 99.3% Identity: 13% PDB header:toxin Chain: D: PDB Molecule:toxin rele3; PDBTitle: crystal structure of relb-rele antitoxin-toxin complex from2 methanococcus jannaschii Phyre2 PDB 3g5o chain C Region: 1 - 82 Aligned: 75 Modelled: 82 Confidence: 99.3% Identity: 25% PDB header:toxin/antitoxin Chain: C: PDB Molecule:uncharacterized protein rv2866; PDBTitle: the crystal structure of the toxin-antitoxin complex relbe2 (rv2865-2 2866) from mycobacterium tuberculosis Phyre2 PDB 1wmi chain A domain 1 Region: 2 - 83 Aligned: 78 Modelled: 82 Confidence: 99.3% Identity: 14% Fold: RelE-like Superfamily: RelE-like Family: RelE-like Phyre2 PDB 3kix chain Y Region: 2 - 82 Aligned: 75 Modelled: 75 Confidence: 98.6% Identity: 15% PDB header:ribosome Chain: Y: PDB Molecule: PDBTitle: structure of rele nuclease bound to the 70s ribosome2 (postcleavage state; part 3 of 4) Phyre2 PDB 3kxe chain B Region: 2 - 82 Aligned: 75 Modelled: 77 Confidence: 97.5% Identity: 16% PDB header:protein binding Chain: B: PDB Molecule:toxin protein pare-1; PDBTitle: a conserved mode of protein recognition and binding in a2 pard-pare toxin-antitoxin complex Phyre2 PDB 2p2e chain A domain 1 Region: 1 - 15 Aligned: 15 Modelled: 15 Confidence: 32.3% Identity: 7% Fold: HesB-like domain Superfamily: HesB-like domain Family: HesB-like domain Phyre2 PDB 2qgo chain A Region: 1 - 15 Aligned: 15 Modelled: 15 Confidence: 31.9% Identity: 7% PDB header:biosynthetic protein Chain: A: PDB Molecule:putative fe-s biosynthesis protein; PDBTitle: crystal structure of a putative fe-s biosynthesis protein from2 lactobacillus acidophilus Phyre2 PDB 3bb9 chain A domain 1 Region: 39 - 67 Aligned: 29 Modelled: 29 Confidence: 24.1% Identity: 7% Fold: Cystatin-like Superfamily: NTF2-like Family: SO0125-like Phyre2 PDB 2qn6 chain A domain 2 Region: 57 - 67 Aligned: 11 Modelled: 11 Confidence: 22.0% Identity: 36% Fold: Elongation factor/aminomethyltransferase common domain Superfamily: EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain Family: EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain Phyre2 PDB 1s0u chain A domain 2 Region: 57 - 67 Aligned: 11 Modelled: 11 Confidence: 21.9% Identity: 45% Fold: Elongation factor/aminomethyltransferase common domain Superfamily: EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain Family: EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain Phyre2 PDB 2l09 chain A Region: 1 - 15 Aligned: 15 Modelled: 15 Confidence: 20.7% Identity: 27% PDB header:oxidoreductase Chain: A: PDB Molecule:asr4154 protein; PDBTitle: solution nmr structure of protein asr4154 from nostoc sp. pcc71202 northeast structural genomics consortium target id nsr143 Phyre2 PDB 1kk1 chain A domain 2 Region: 57 - 67 Aligned: 11 Modelled: 11 Confidence: 20.5% Identity: 45% Fold: Elongation factor/aminomethyltransferase common domain Superfamily: EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain Family: EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain Phyre2 PDB 3e9v chain A domain 1 Region: 14 - 62 Aligned: 47 Modelled: 49 Confidence: 17.7% Identity: 15% Fold: BTG domain-like Superfamily: BTG domain-like Family: BTG domain-like Phyre2 PDB 1wmv chain A Region: 41 - 68 Aligned: 28 Modelled: 28 Confidence: 16.2% Identity: 18% PDB header:oxidoreductase, apoptosis Chain: A: PDB Molecule:ww domain containing oxidoreductase; PDBTitle: solution structure of the second ww domain of wwox Phyre2 PDB 2dog chain A Region: 42 - 83 Aligned: 41 Modelled: 42 Confidence: 14.5% Identity: 22% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:probable 16s rrna-processing protein rimm; PDBTitle: solution structure of the n-terminal domain of rimm from thermus2 thermophilus hb8 Phyre2 1 d2a6sa1 2 c3oeiH_ 3 d1z8ma1 4 c2otrA_ 5 c2kheA_ 6 c3bpqD_ 7 c3g5oC_ 8 d1wmia1 9 c3kixy_ 10 c3kxeB_ 11 d2p2ea1 12 c2qgoA_ 13 d3bb9a1 14 d2qn6a2 15 d1s0ua2 16 c2l09A_ 17 d1kk1a2 18 d3e9va1 19 c1wmvA_ 20 c2dogA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2a6sa1 100.0 100 Fold:RelE-like Superfamily:RelE-like Family:YoeB/Txe-like 2 c3oeiH_ 100.0 46 PDB header:toxin, protein binding Chain: H: PDB Molecule:relk (toxin rv3358); PDBTitle: crystal structure of mycobacterium tuberculosis reljk (rv3357-rv3358-2 relbe3) 3 d1z8ma1 99.8 19 Fold:RelE-like Superfamily:RelE-like Family:RelE-like 4 c2otrA_ 99.8 18 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein hp0892; PDBTitle: solution structure of conserved hypothetical protein hp0892 from2 helicobacter pylori 5 c2kheA_ 99.4 18 PDB header:hydrolase Chain: A: PDB Molecule:toxin-like protein; PDBTitle: solution structure of the bacterial toxin rele from thermus2 thermophilus hb8 6 c3bpqD_ 99.3 13 PDB header:toxin Chain: D: PDB Molecule:toxin rele3; PDBTitle: crystal structure of relb-rele antitoxin-toxin complex from2 methanococcus jannaschii 7 c3g5oC_ 99.3 25 PDB header:toxin/antitoxin Chain: C: PDB Molecule:uncharacterized protein rv2866; PDBTitle: the crystal structure of the toxin-antitoxin complex relbe2 (rv2865-2 2866) from mycobacterium tuberculosis 8 d1wmia1 99.3 14 Fold:RelE-like Superfamily:RelE-like Family:RelE-like 9 c3kixy_ 98.6 15 PDB header:ribosome Chain: Y: PDB Molecule: PDBTitle: structure of rele nuclease bound to the 70s ribosome2 (postcleavage state; part 3 of 4) 10 c3kxeB_ 97.5 16 PDB header:protein binding Chain: B: PDB Molecule:toxin protein pare-1; PDBTitle: a conserved mode of protein recognition and binding in a2 pard-pare toxin-antitoxin complex 11 d2p2ea1 32.3 7 Fold:HesB-like domain Superfamily:HesB-like domain Family:HesB-like domain 12 c2qgoA_ 31.9 7 PDB header:biosynthetic protein Chain: A: PDB Molecule:putative fe-s biosynthesis protein; PDBTitle: crystal structure of a putative fe-s biosynthesis protein from2 lactobacillus acidophilus 13 d3bb9a1 24.1 7 Fold:Cystatin-like Superfamily:NTF2-like Family:SO0125-like 14 d2qn6a2 22.0 36 Fold:Elongation factor/aminomethyltransferase common domain Superfamily:EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain Family:EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain 15 d1s0ua2 21.9 45 Fold:Elongation factor/aminomethyltransferase common domain Superfamily:EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain Family:EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain 16 c2l09A_ 20.7 27 PDB header:oxidoreductase Chain: A: PDB Molecule:asr4154 protein; PDBTitle: solution nmr structure of protein asr4154 from nostoc sp. pcc71202 northeast structural genomics consortium target id nsr143 17 d1kk1a2 20.5 45 Fold:Elongation factor/aminomethyltransferase common domain Superfamily:EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain Family:EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain 18 d3e9va1 17.7 15 Fold:BTG domain-like Superfamily:BTG domain-like Family:BTG domain-like 19 c1wmvA_ 16.2 18 PDB header:oxidoreductase, apoptosis Chain: A: PDB Molecule:ww domain containing oxidoreductase; PDBTitle: solution structure of the second ww domain of wwox 20 c2dogA_ 14.5 22 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:probable 16s rrna-processing protein rimm; PDBTitle: solution structure of the n-terminal domain of rimm from thermus2 thermophilus hb8 21 d2ux0a1 not modelled 13.6 26 Fold:Cystatin-like Superfamily:NTF2-like Family:Association domain of calcium/calmodulin-dependent protein kinase type II alpha subunit, CAMK2A 22 c2kruA_ not modelled 13.3 21 PDB header:oxidoreductase Chain: A: PDB Molecule:light-independent protochlorophyllide reductase PDBTitle: solution nmr structure of the pcp_red domain of light-2 independent protochlorophyllide reductase subunit b from3 chlorobium tepidum. northeast structural genomics4 consortium target ctr69a (casp target) 23 c2apnA_ not modelled 13.1 5 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:protein hi1723; PDBTitle: hi1723 solution structure 24 d1khba2 not modelled 13.0 28 Fold:PEP carboxykinase N-terminal domain Superfamily:PEP carboxykinase N-terminal domain Family:PEP carboxykinase N-terminal domain 25 d2z15a1 not modelled 11.5 29 Fold:BTG domain-like Superfamily:BTG domain-like Family:BTG domain-like 26 c3sjaG_ not modelled 10.3 16 PDB header:hydrolase/transport protein Chain: G: PDB Molecule:golgi to er traffic protein 1; PDBTitle: crystal structure of s. cerevisiae get3 in the open state in complex2 with get1 cytosolic domain 27 d2f86b1 not modelled 8.6 19 Fold:Cystatin-like Superfamily:NTF2-like Family:Association domain of calcium/calmodulin-dependent protein kinase type II alpha subunit, CAMK2A 28 d1hkxa_ not modelled 8.5 22 Fold:Cystatin-like Superfamily:NTF2-like Family:Association domain of calcium/calmodulin-dependent protein kinase type II alpha subunit, CAMK2A 29 c2l4jA_ not modelled 8.4 29 PDB header:transcription Chain: A: PDB Molecule:yes-associated protein 2 (yap2); PDBTitle: yap ww2 30 c3hx8A_ not modelled 8.4 10 PDB header:isomerase Chain: A: PDB Molecule:putative ketosteroid isomerase; PDBTitle: crystal structure of putative ketosteroid isomerase2 (np_103587.1) from mesorhizobium loti at 1.45 a resolution 31 d1jtma_ not modelled 8.2 13 Fold:Lysozyme-like Superfamily:Lysozyme-like Family:Phage lysozyme 32 c3sjbC_ not modelled 8.1 14 PDB header:hydrolase/transport protein Chain: C: PDB Molecule:golgi to er traffic protein 1; PDBTitle: crystal structure of s. cerevisiae get3 in the open state in complex2 with get1 cytosolic domain 33 d1p5ca_ not modelled 7.8 11 Fold:Lysozyme-like Superfamily:Lysozyme-like Family:Phage lysozyme 34 c3q9cF_ not modelled 7.4 15 PDB header:hydrolase Chain: F: PDB Molecule:acetylpolyamine amidohydrolase; PDBTitle: crystal structure of h159a apah complexed with n8-acetylspermidine 35 c3robC_ not modelled 7.1 20 PDB header:structural genomics, unknown function Chain: C: PDB Molecule:uncharacterized conserved protein; PDBTitle: the crystal structure of a conserved protein from planctomyces2 limnophilus dsm 3776 36 c2qeyA_ not modelled 6.5 29 PDB header:lyase Chain: A: PDB Molecule:phosphoenolpyruvate carboxykinase, cytosolic [gtp]; PDBTitle: rat cytosolic pepck in complex with gtp 37 d1nwba_ not modelled 5.7 0 Fold:HesB-like domain Superfamily:HesB-like domain Family:HesB-like domain 38 c2fh0A_ not modelled 5.6 6 PDB header:unknown function Chain: A: PDB Molecule:hypothetical 16.0 kda protein in abf2-chl12 PDBTitle: nmr ensemble of the yeast saccharomyces cerevisiae protein2 ymr074cp core region 39 d2jb0b1 not modelled 5.2 11 Fold:His-Me finger endonucleases Superfamily:His-Me finger endonucleases Family:HNH-motif 40 d1eija_ not modelled 5.2 19 Fold:RuvA C-terminal domain-like Superfamily:Double-stranded DNA-binding domain Family:Double-stranded DNA-binding domain 41 d1tbga_ not modelled 5.2 12 Fold:7-bladed beta-propeller Superfamily:WD40 repeat-like Family:WD40-repeat 42 c2awyB_ not modelled 5.1 30 PDB header:oxygen storage/transport Chain: B: PDB Molecule:hemerythrin-like domain protein dcrh; PDBTitle: met-dcrh-hr

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0