Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	D	E	I	D	A	M	A	L	Y	R	A	W	Q	Q	L	D	N	G	S	C	A	Q	I	R	R	V	S	E	P	D	E	L	R	D	I	P	A	F	Y	R	L	V	Q	P	F	G	W	E	N	P	R	H	Q	Q	A	L	L	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?										?	?	?	?	?	?	?	?					?				?																?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		M	V	F	C	L	S	A	G	K	N	V	I	R	H	Q	D	K	K	S	E	Q	T	T	G	I	S	L	G	R	A	L	A	N	S	G	R	I	N	E	R	R	I	F	Q	L	I	R	A	D	R	T	A	D	M	V	Q	L	R	R	L
Secondary structure
SS confidence
Disorder														?		?	?	?	?	?	?	?	?	?	?							?	?	?	?	?	?	?	?														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160
Sequence		L	T	H	A	E	P	V	L	D	W	P	L	M	A	R	M	L	T	W	W	G	K	R	E	R	Q	Q	L	L	E	D	F	V	L	T	T	N	K	N	A
Secondary structure
SS confidence
Disorder					?																																?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2zca chain B Region: 6 - 156 Aligned: 147 Modelled: 151 Confidence: 100.0% Identity: 16% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative uncharacterized protein tthb189; PDBTitle: crystal structure of tthb189, a crispr-associated protein,2 cse2 family from thermus thermophilus hb8 Phyre2 PDB 1k3k chain A Region: 98 - 139 Aligned: 42 Modelled: 42 Confidence: 19.6% Identity: 19% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 1shz chain F Region: 103 - 155 Aligned: 51 Modelled: 53 Confidence: 9.1% Identity: 16% PDB header:signaling protein Chain: F: PDB Molecule:rho guanine nucleotide exchange factor 1; PDBTitle: crystal structure of the p115rhogef rgrgs domain in a2 complex with galpha(13):galpha(i1) chimera Phyre2 PDB 1h8b chain A Region: 1 - 37 Aligned: 37 Modelled: 37 Confidence: 9.1% Identity: 24% Fold: EF Hand-like Superfamily: EF-hand Family: EF-hand modules in multidomain proteins Phyre2 PDB 1j55 chain A Region: 92 - 160 Aligned: 61 Modelled: 69 Confidence: 7.8% Identity: 10% Fold: EF Hand-like Superfamily: EF-hand Family: S100 proteins Phyre2 PDB 1oh0 chain A Region: 1 - 38 Aligned: 38 Modelled: 38 Confidence: 7.1% Identity: 16% Fold: Cystatin-like Superfamily: NTF2-like Family: Ketosteroid isomerase-like Phyre2 PDB 1ysy chain A domain 1 Region: 105 - 138 Aligned: 34 Modelled: 34 Confidence: 6.6% Identity: 15% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Coronavirus NSP7-like Family: Coronavirus NSP7-like Phyre2 PDB 2kua chain A Region: 46 - 139 Aligned: 94 Modelled: 94 Confidence: 6.4% Identity: 4% PDB header:apoptosis Chain: A: PDB Molecule:bcl-2-like protein 10; PDBTitle: solution structure of a divergent bcl-2 protein Phyre2 PDB 1ohp chain A domain 1 Region: 1 - 38 Aligned: 38 Modelled: 38 Confidence: 6.1% Identity: 18% Fold: Cystatin-like Superfamily: NTF2-like Family: Ketosteroid isomerase-like Phyre2 1 c2zcaB_ 2 d1k3ka_ 3 c1shzF_ 4 d1h8ba_ 5 d1j55a_ 6 d1oh0a_ 7 d1ysya1 8 c2kuaA_ 9 d1ohpa1

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2zcaB_ 100.0 16 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative uncharacterized protein tthb189; PDBTitle: crystal structure of tthb189, a crispr-associated protein,2 cse2 family from thermus thermophilus hb8 2 d1k3ka_ 19.6 19 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 3 c1shzF_ 9.1 16 PDB header:signaling protein Chain: F: PDB Molecule:rho guanine nucleotide exchange factor 1; PDBTitle: crystal structure of the p115rhogef rgrgs domain in a2 complex with galpha(13):galpha(i1) chimera 4 d1h8ba_ 9.1 24 Fold:EF Hand-like Superfamily:EF-hand Family:EF-hand modules in multidomain proteins 5 d1j55a_ 7.8 10 Fold:EF Hand-like Superfamily:EF-hand Family:S100 proteins 6 d1oh0a_ 7.1 16 Fold:Cystatin-like Superfamily:NTF2-like Family:Ketosteroid isomerase-like 7 d1ysya1 6.6 15 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Coronavirus NSP7-like Family:Coronavirus NSP7-like 8 c2kuaA_ 6.4 4 PDB header:apoptosis Chain: A: PDB Molecule:bcl-2-like protein 10; PDBTitle: solution structure of a divergent bcl-2 protein 9 d1ohpa1 6.1 18 Fold:Cystatin-like Superfamily:NTF2-like Family:Ketosteroid isomerase-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0