Job Description P37677 Confidence 100.00% Date Thu Jan 5 11:56:51 GMT 2012 Rank 14 Aligned Residues 463 % Identity 22% Template c3ezwD_ PDB info PDB header:transferase Chain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices Resolution 2.00 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.
Predicted Secondary structure
Query SS confidence
Query Sequence		T	Q	Y	W	L	G	L	D	C	G	G	S	W	L	K	A	G	L	Y	D	R	E	G	R	E	A	G	V	Q	R	L	P	L	C	A	L	S	P	Q	P	G	W	A	E	R	D	M	A	E	L	W	Q	C	C	M	A	V	I	R	A	L	L	T	H	S	G	V	S	G	E	Q	I	V	G	I	G	I	S	A	Q
Query Conservation
Alig confidence
Template Conservation
Template Sequence		K	K	Y	I	V	A	L	D	Q	G	T	T	S	S	R	A	V	V	M	D	H	D	A	N	I	I	S	V	S	Q	R	E	F	E	Q	I	Y	P	K	P	G	W	V	E	H	D	P	M	E	I	W	A	T	Q	S	S	T	L	V	E	V	L	A	K	A	D	I	S	S	D	Q	I	A	A	I	G	I	T	N	Q
Template Known Secondary structure												S	S									T	T														B			S	T	T																								T	T			G	G	G
Template Predicted Secondary structure
Template SS confidence
		3	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.
		82	.	.	.	.	.	.	.	90		.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.					.
Predicted Secondary structure											.																																																																		.	.	.	.
Query SS confidence											.																																																																		.	.	.	.
Query Sequence		G	K	G	L	F	L	L	D	K	.	N	D	K	P	L	G	N	A	I	L	S	S	D	R	R	A	M	E	I	V	R	R	W	Q	E	D	G	I	P	E	K	L	Y	P	L	T	R	Q	T	L	W	T	G	H	P	V	S	L	L	R	W	L	K	E	H	E	P	E	R	Y	A	Q	I	G	C	.	.	.	.	V
Query Conservation											.																																																																		.	.	.	.
Alig confidence											.																																																																		.	.	.	.
Template Conservation
Template Sequence		R	E	T	T	I	V	W	E	K	E	T	G	K	P	I	Y	N	A	I	V	W	Q	C	R	R	T	A	E	I	C	E	H	L	K	R	D	G	L	E	D	Y	I	R	S	N	T	G	L	V	I	D	P	Y	F	S	G	T	K	V	K	W	I	L	D	H	V	E	G	S	R	E	R	A	R	R	G	E	L	L	F
Template Known Secondary structure		S	S		B					T	T	T			B	S	S					T	T			T	T										T	T										S			S	S	T	T	S												S	T	T							T	T	S
Template Predicted Secondary structure
Template SS confidence
		83	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.
		157	.	.	160	.	.	.	.	.	.	.	.	.			170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.
Predicted Secondary structure															.	.
Query SS confidence															.	.
Query Sequence		M	M	T	H	D	Y	L	R	W	C	L	T	G	.	.	V	K	G	C	E	E	S	N	I	S	E	S	N	L	Y	N	M	S	L	G	E	Y	D	P	C	L	T	D	W	L	G	I	A	E	I	N	H	A	L	P	P	V	V	G	S	A	E	I	C	G	E	I	T	A	Q	T	A	A	L	T	G	L	K	A	G
Query Conservation															.	.
Alig confidence															.	.																																			.	.																		.	.	.	.	.	.	.	.	.	.	.
Template Conservation																																																			.	.																		.	.	.	.	.	.	.	.	.	.	.
Template Sequence		G	T	V	D	T	W	L	I	W	K	M	T	Q	G	R	V	H	V	T	D	Y	T	N	A	S	R	T	M	L	F	N	I	H	T	L	D	W	D	D	K	M	L	E	V	L	D	I	P	R	.	.	E	M	L	P	E	V	R	R	S	S	E	V	Y	G	Q	T	N	.	.	.	.	.	.	.	.	.	.	.	R
Template Known Secondary structure													T	T	T	S										G	G	G	S	S			T	T	T	T										T	T			G	.	.	G	G							S									.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																																																			.	.																		.	.	.	.	.	.	.	.	.	.	.
Template SS confidence
		163	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.			.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.												.
		235	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.				.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.
Predicted Secondary structure																																																													.	.	.
Query SS confidence																																																													.	.	.
Query Sequence		T	P	V	V	G	G	L	F	D	V	V	S	T	A	L	C	A	G	I	E	D	E	F	T	L	N	A	V	M	G	T	W	A	V	T	S	G	I	T	R	G	L	R	D	G	E	A	H	P	Y	V	Y	G	R	Y	V	N	D	G	.	.	.	E	F	I	V	H	E	A	S	P	T	S	S	G	N	L	E	W	F
Query Conservation																																																													.	.	.
Alig confidence																																																													.	.	.
Template Conservation
Template Sequence		I	P	I	S	G	I	A	G	D	Q	Q	A	A	L	F	G	Q	L	C	V	K	E	G	M	A	K	N	T	Y	G	T	G	C	F	M	L	M	N	T	G	E	K	A	V	K	S	E	N	G	L	L	T	T	I	A	C	G	P	T	G	E	V	N	Y	A	L	E	G	A	V	F	M	A	G	A	S	I	Q	W	L
Template Known Secondary structure																		T	T			S	T	T							S	S	S								T	T	S					S	S	S									T	T	S											S		S
Template Predicted Secondary structure
Template SS confidence
		237	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.
		312	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.				.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.
Predicted Secondary structure																																										.	.	.
Query SS confidence																																										.	.	.
Query Sequence		T	A	Q	W	G	E	I	S	F	D	E	I	N	Q	A	V	A	S	L	P	K	A	G	G	D	L	F	F	L	P	F	L	Y	G	S	N	A	G	L	E	.	.	.	M	T	S	G	F	Y	G	M	Q	A	I	H	T	R	A	H	L	L	Q	A	I	Y	E	G	V	V	F	S	H	M	T	H	L	N	R	M	R
Query Conservation																																										.	.	.
Alig confidence										.	.	.	.																													.	.	.
Template Conservation										.	.	.	.
Template Sequence		R	D	E	M	K	L	I	D	.	.	.	.	S	E	Y	F	A	T	K	V	Q	N	T	N	G	V	Y	V	V	P	A	F	T	G	L	G	A	P	Y	W	D	P	Y	A	R	G	A	I	F	G	L	T	R	G	V	N	A	N	H	I	I	R	A	T	L	E	S	I	A	Y	Q	T	R	D	V	L	E	A	M	Q
Template Known Secondary structure				T	S					.	.	.	.							T	S	S	S		T	T							T	T		B		T	T	T		B	T	T										T	T
Template Predicted Secondary structure										.	.	.	.
Template SS confidence
		317	.	.	320	.	.	.	.					.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.
		389		390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.
Predicted Secondary structure			.
Query SS confidence			.
Query Sequence		E	.	R	F	T	D	V	H	T	L	R	V	T	G	G	P	A	H	S	D	V	W	M	Q	M	L	A	D	V	S	G	L	R	I	E	L	P	Q	V	E	E	T	G	C	F	G	A	A	L	A	A	R	V	G	T	G	V	Y	H	N	F	S	E	A	Q	R	D	L	R	H	P	V	R	T	L	L	P	D	M	T
Query Conservation			.
Alig confidence			.																																			.	.	.																									.	.	.	.
Template Conservation																																						.	.	.																									.	.	.	.
Template Sequence		A	D	S	G	I	R	L	H	A	L	R	V	D	G	G	A	V	A	N	N	F	L	M	Q	F	Q	S	D	I	L	G	T	R	V	E	R	.	.	.	P	E	V	T	A	L	G	A	A	Y	L	A	G	L	A	V	G	F	W	Q	N	L	D	E	.	.	.	.	L	Q	E	I	E	R	E	F	R	P	G	I	E
Template Known Secondary structure									S						S	G	G	G	G													T	S					.	.	.																	T	S	S	S					.	.	.	.		T										S
Template Predicted Secondary structure																																						.	.	.																									.	.	.	.
Template SS confidence
		397	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.				.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.					.	.	.	460	.	.	.	.	.	.	.	.	.
		468	.	470	.	.	.	.	.	.	.	.	.	480	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		A	H	Q	L	Y	Q	K	K	Y	Q	R	Y	Q	H	L	I	A	A	L	Q	G	F
Query Conservation
Alig confidence									.
Template Conservation									.
Template Sequence		T	T	E	R	N	Y	R	.	Y	A	G	W	K	K	A	V	K	R	A	M	A	W
Template Known Secondary structure									.												S	S
Template Predicted Secondary structure									.
Template SS confidence
		476	.	.	.	480	.	.		.	.	.	.	.	.	.	490	.	.	.	.	.	.