Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	I	V	D	Q	Q	T	F	R	D	A	M	S	C	M	G	A	A	V	N	I	I	T	T	D	G	P	A	G	R	A	G	F	T	A	S	A	V	C	S	V	T	D	T	P	P	T	L	L	V	C	L	N	R	G	A	S	V	W
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	A	F	N	E	N	R	T	L	C	V	N	T	L	S	A	G	Q	E	P	L	S	N	L	F	G	G	K	T	P	M	E	H	R	F	A	A	A	R	W	Q	T	G	V	T	G	C	P	Q	L	E	E	A	L	V	S	F	D	C	R
Secondary structure
SS confidence
Disorder																												?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.
Sequence		I	S	Q	V	V	S	V	G	T	H	D	I	L	F	C	A	I	E	A	I	H	R	H	T	T	P	Y	G	L	V	W	F	D	R	S	Y	H	A	L	M	R	P	A	C
Secondary structure
SS confidence
Disorder																							?	?															?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2r0x chain A Region: 4 - 159 Aligned: 156 Modelled: 156 Confidence: 100.0% Identity: 50% PDB header:oxidoreductase Chain: A: PDB Molecule:possible flavin reductase; PDBTitle: crystal structure of a putative flavin reductase (ycdh, hs_1225) from2 haemophilus somnus 129pt at 1.06 a resolution Phyre2 PDB 3k87 chain B Region: 2 - 161 Aligned: 160 Modelled: 160 Confidence: 100.0% Identity: 41% PDB header:oxidoreductase Chain: B: PDB Molecule:chlorophenol-4-monooxygenase component 1; PDBTitle: crystal structure of nadh:fad oxidoreductase (tftc) - fad2 complex Phyre2 PDB 3rh7 chain A Region: 2 - 160 Aligned: 156 Modelled: 159 Confidence: 100.0% Identity: 31% PDB header:oxidoreductase Chain: A: PDB Molecule:hypothetical oxidoreductase; PDBTitle: crystal structure of a hypothetical oxidoreductase (sma0793) from2 sinorhizobium meliloti 1021 at 3.00 a resolution Phyre2 PDB 3cb0 chain B Region: 1 - 159 Aligned: 159 Modelled: 159 Confidence: 100.0% Identity: 39% PDB header:oxidoreductase Chain: B: PDB Molecule:4-hydroxyphenylacetate 3-monooxygenase; PDBTitle: cobr Phyre2 PDB 3nfw chain B Region: 4 - 163 Aligned: 158 Modelled: 159 Confidence: 100.0% Identity: 25% PDB header:oxidoreductase Chain: B: PDB Molecule:nitrilotriacetate monooxygenase component b; PDBTitle: crystal structure of nitrilotriacetate monooxygenase component b2 (a0r521 homolog) from mycobacterium thermoresistibile Phyre2 PDB 3pft chain A Region: 4 - 160 Aligned: 155 Modelled: 157 Confidence: 100.0% Identity: 19% PDB header:oxidoreductase Chain: A: PDB Molecule:flavin reductase; PDBTitle: crystal structure of untagged c54a mutant flavin reductase (dszd) in2 complex with fmn from mycobacterium goodii Phyre2 PDB 2qck chain A Region: 6 - 161 Aligned: 153 Modelled: 156 Confidence: 100.0% Identity: 22% PDB header:oxidoreductase Chain: A: PDB Molecule:flavin reductase domain protein; PDBTitle: crystal structure of flavin reductase domain protein (yp_831077.1)2 from arthrobacter sp. fb24 at 1.90 a resolution Phyre2 PDB 2d38 chain A Region: 6 - 161 Aligned: 153 Modelled: 156 Confidence: 100.0% Identity: 20% PDB header:oxidoreductase Chain: A: PDB Molecule:hypothetical nadh-dependent fmn oxidoreductase; PDBTitle: the crystal structure of flavin reductase hpac complexed with nadp+ Phyre2 PDB 2ecr chain A Region: 6 - 163 Aligned: 148 Modelled: 158 Confidence: 100.0% Identity: 26% PDB header:oxidoreductase Chain: A: PDB Molecule:flavin reductase component (hpac) of 4-hydroxyphenylacetate PDBTitle: crystal structure of the ligand-free form of the flavin reductase2 component (hpac) of 4-hydroxyphenylacetate 3-monooxygenase Phyre2 PDB 1wgb chain A Region: 5 - 157 Aligned: 153 Modelled: 153 Confidence: 100.0% Identity: 18% Fold: Split barrel-like Superfamily: FMN-binding split barrel Family: NADH:FMN oxidoreductase-like Phyre2 PDB 1rz0 chain A Region: 5 - 161 Aligned: 152 Modelled: 157 Confidence: 100.0% Identity: 27% Fold: Split barrel-like Superfamily: FMN-binding split barrel Family: NADH:FMN oxidoreductase-like Phyre2 PDB 1i0r chain A Region: 4 - 156 Aligned: 149 Modelled: 153 Confidence: 100.0% Identity: 21% Fold: Split barrel-like Superfamily: FMN-binding split barrel Family: NADH:FMN oxidoreductase-like Phyre2 PDB 3bnk chain B Region: 13 - 162 Aligned: 150 Modelled: 150 Confidence: 100.0% Identity: 18% PDB header:electron transport Chain: B: PDB Molecule:flavoredoxin; PDBTitle: x-ray crystal structure of flavoredoxin from methanosarcina2 acetivorans Phyre2 PDB 2r6v chain A Region: 15 - 162 Aligned: 148 Modelled: 148 Confidence: 100.0% Identity: 14% PDB header:flavoprotein Chain: A: PDB Molecule:uncharacterized protein ph0856; PDBTitle: crystal structure of fmn-binding protein (np_142786.1) from pyrococcus2 horikoshii at 1.35 a resolution Phyre2 PDB 1eje chain A Region: 1 - 162 Aligned: 160 Modelled: 162 Confidence: 100.0% Identity: 13% Fold: Split barrel-like Superfamily: FMN-binding split barrel Family: NADH:FMN oxidoreductase-like Phyre2 PDB 1usc chain A Region: 12 - 158 Aligned: 147 Modelled: 147 Confidence: 100.0% Identity: 16% Fold: Split barrel-like Superfamily: FMN-binding split barrel Family: NADH:FMN oxidoreductase-like Phyre2 PDB 3bpk chain B Region: 6 - 161 Aligned: 156 Modelled: 156 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: B: PDB Molecule:nitrilotriacetate monooxygenase component b; PDBTitle: crystal structure of nitrilotriacetate monooxygenase2 component b from bacillus cereus Phyre2 PDB 3e4v chain A Region: 6 - 161 Aligned: 153 Modelled: 156 Confidence: 100.0% Identity: 12% PDB header:flavoprotein Chain: A: PDB Molecule:nadh:fmn oxidoreductase like protein; PDBTitle: crystal structure of nadh:fmn oxidoreductase like protein in complex2 with fmn (yp_544701.1) from methylobacillus flagellatus kt at 1.40 a3 resolution Phyre2 PDB 3hmz chain A Region: 4 - 162 Aligned: 159 Modelled: 159 Confidence: 100.0% Identity: 11% PDB header:oxidoreductase Chain: A: PDB Molecule:flavin reductase domain protein, fmn-binding; PDBTitle: crystal structure of a fmn-binding domain of flavin reductases-like2 enzyme (sbal_0626) from shewanella baltica os155 at 1.50 a resolution Phyre2 PDB 2d5m chain A Region: 4 - 162 Aligned: 155 Modelled: 159 Confidence: 100.0% Identity: 13% PDB header:electron transport Chain: A: PDB Molecule:flavoredoxin; PDBTitle: flavoredoxin of desulfovibrio vulgaris (miyazaki f) Phyre2 1 c2r0xA_ 2 c3k87B_ 3 c3rh7A_ 4 c3cb0B_ 5 c3nfwB_ 6 c3pftA_ 7 c2qckA_ 8 c2d38A_ 9 c2ecrA_ 10 d1wgba_ 11 d1rz0a_ 12 d1i0ra_ 13 c3bnkB_ 14 c2r6vA_ 15 d1ejea_ 16 d1usca_ 17 c3bpkB_ 18 c3e4vA_ 19 c3hmzA_ 20 c2d5mA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2r0xA_ 100.0 50 PDB header:oxidoreductase Chain: A: PDB Molecule:possible flavin reductase; PDBTitle: crystal structure of a putative flavin reductase (ycdh, hs_1225) from2 haemophilus somnus 129pt at 1.06 a resolution 2 c3k87B_ 100.0 41 PDB header:oxidoreductase Chain: B: PDB Molecule:chlorophenol-4-monooxygenase component 1; PDBTitle: crystal structure of nadh:fad oxidoreductase (tftc) - fad2 complex 3 c3rh7A_ 100.0 31 PDB header:oxidoreductase Chain: A: PDB Molecule:hypothetical oxidoreductase; PDBTitle: crystal structure of a hypothetical oxidoreductase (sma0793) from2 sinorhizobium meliloti 1021 at 3.00 a resolution 4 c3cb0B_ 100.0 39 PDB header:oxidoreductase Chain: B: PDB Molecule:4-hydroxyphenylacetate 3-monooxygenase; PDBTitle: cobr 5 c3nfwB_ 100.0 25 PDB header:oxidoreductase Chain: B: PDB Molecule:nitrilotriacetate monooxygenase component b; PDBTitle: crystal structure of nitrilotriacetate monooxygenase component b2 (a0r521 homolog) from mycobacterium thermoresistibile 6 c3pftA_ 100.0 19 PDB header:oxidoreductase Chain: A: PDB Molecule:flavin reductase; PDBTitle: crystal structure of untagged c54a mutant flavin reductase (dszd) in2 complex with fmn from mycobacterium goodii 7 c2qckA_ 100.0 22 PDB header:oxidoreductase Chain: A: PDB Molecule:flavin reductase domain protein; PDBTitle: crystal structure of flavin reductase domain protein (yp_831077.1)2 from arthrobacter sp. fb24 at 1.90 a resolution 8 c2d38A_ 100.0 20 PDB header:oxidoreductase Chain: A: PDB Molecule:hypothetical nadh-dependent fmn oxidoreductase; PDBTitle: the crystal structure of flavin reductase hpac complexed with nadp+ 9 c2ecrA_ 100.0 26 PDB header:oxidoreductase Chain: A: PDB Molecule:flavin reductase component (hpac) of 4-hydroxyphenylacetate PDBTitle: crystal structure of the ligand-free form of the flavin reductase2 component (hpac) of 4-hydroxyphenylacetate 3-monooxygenase 10 d1wgba_ 100.0 18 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:NADH:FMN oxidoreductase-like 11 d1rz0a_ 100.0 27 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:NADH:FMN oxidoreductase-like 12 d1i0ra_ 100.0 21 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:NADH:FMN oxidoreductase-like 13 c3bnkB_ 100.0 18 PDB header:electron transport Chain: B: PDB Molecule:flavoredoxin; PDBTitle: x-ray crystal structure of flavoredoxin from methanosarcina2 acetivorans 14 c2r6vA_ 100.0 14 PDB header:flavoprotein Chain: A: PDB Molecule:uncharacterized protein ph0856; PDBTitle: crystal structure of fmn-binding protein (np_142786.1) from pyrococcus2 horikoshii at 1.35 a resolution 15 d1ejea_ 100.0 13 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:NADH:FMN oxidoreductase-like 16 d1usca_ 100.0 16 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:NADH:FMN oxidoreductase-like 17 c3bpkB_ 100.0 17 PDB header:oxidoreductase Chain: B: PDB Molecule:nitrilotriacetate monooxygenase component b; PDBTitle: crystal structure of nitrilotriacetate monooxygenase2 component b from bacillus cereus 18 c3e4vA_ 100.0 12 PDB header:flavoprotein Chain: A: PDB Molecule:nadh:fmn oxidoreductase like protein; PDBTitle: crystal structure of nadh:fmn oxidoreductase like protein in complex2 with fmn (yp_544701.1) from methylobacillus flagellatus kt at 1.40 a3 resolution 19 c3hmzA_ 100.0 11 PDB header:oxidoreductase Chain: A: PDB Molecule:flavin reductase domain protein, fmn-binding; PDBTitle: crystal structure of a fmn-binding domain of flavin reductases-like2 enzyme (sbal_0626) from shewanella baltica os155 at 1.50 a resolution 20 c2d5mA_ 100.0 13 PDB header:electron transport Chain: A: PDB Molecule:flavoredoxin; PDBTitle: flavoredoxin of desulfovibrio vulgaris (miyazaki f) 21 c3fgeA_ not modelled 100.0 11 PDB header:oxidoreductase Chain: A: PDB Molecule:putative flavin reductase with split barrel domain; PDBTitle: crystal structure of putative flavin reductase with split barrel2 domain (yp_750721.1) from shewanella frigidimarina ncimb 400 at 1.743 a resolution 22 c3b5mD_ not modelled 99.9 13 PDB header:structural genomics, unknown function Chain: D: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of conserved uncharacterized protein from2 rhodopirellula baltica 23 d2imla1 not modelled 99.3 11 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:MTH863-like 24 c2ptfB_ not modelled 98.6 14 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein mth_863; PDBTitle: crystal structure of protein mth_863 from methanobacterium2 thermoautotrophicum bound to fmn 25 d2nr4a1 not modelled 98.6 16 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:MTH863-like 26 d2ptfa1 not modelled 98.5 15 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:MTH863-like 27 d1flma_ not modelled 95.3 12 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 28 c2q9kA_ not modelled 95.0 14 PDB header:oxidoreductase Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a putative oxidoreductase (exig_1997) from2 exiguobacterium sibiricum 255-15 at 1.59 a resolution 29 c2htdB_ not modelled 88.3 6 PDB header:oxidoreductase Chain: B: PDB Molecule:predicted flavin-nucleotide-binding protein from cog3576 PDBTitle: crystal structure of a putative pyridoxamine 5'-phosphate oxidase2 (ldb0262) from lactobacillus delbrueckii subsp. at 1.60 a resolution 30 d1vl7a_ not modelled 78.9 18 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 31 d2hq7a1 not modelled 70.0 10 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 32 c2re7A_ not modelled 60.5 7 PDB header:oxidoreductase Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a pyridoxamine 5'-phosphate oxidase related2 protein (psyc_0186) from psychrobacter arcticus 273-4 at 2.50 a3 resolution 33 d1rfea_ not modelled 60.2 12 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 34 d2asfa1 not modelled 54.1 8 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 35 d2htia1 not modelled 51.9 8 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 36 c2htiA_ not modelled 51.9 8 PDB header:fmn-binding protein Chain: A: PDB Molecule:bh0577 protein; PDBTitle: crystal structure of a flavin-nucleotide-binding protein (bh_0577)2 from bacillus halodurans at 2.50 a resolution 37 c3ec6A_ not modelled 46.4 11 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:general stress protein 26; PDBTitle: crystal structure of the general stress protein 26 from bacillus2 anthracis str. sterne 38 d2fhqa1 not modelled 46.4 9 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 39 c3dmbA_ not modelled 43.2 11 PDB header:oxidoreductase Chain: A: PDB Molecule:putative general stress protein 26 with a pnp-oxidase like PDBTitle: crystal structure of a putative general stress family protein2 (xcc2264) from xanthomonas campestris pv. campestris at 2.30 a3 resolution 40 c3db0B_ not modelled 36.1 11 PDB header:oxidoreductase Chain: B: PDB Molecule:lin2891 protein; PDBTitle: crystal structure of putative pyridoxamine 5'-phosphate oxidase2 (np_472219.1) from listeria innocua at 2.00 a resolution 41 c3f7eB_ not modelled 34.6 10 PDB header:unknown function Chain: B: PDB Molecule:pyridoxamine 5'-phosphate oxidase-related, fmn- PDBTitle: msmeg_3380 f420 reductase 42 d1w9aa_ not modelled 24.7 8 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 43 c3fkhB_ not modelled 22.5 11 PDB header:oxidoreductase Chain: B: PDB Molecule:putative pyridoxamine 5'-phosphate oxidase; PDBTitle: crystal structure of putative pyridoxamine 5'-phosphate oxidase2 (np_601736.1) from corynebacterium glutamicum atcc 13032 kitasato at3 2.51 a resolution 44 d1xzwa1 not modelled 19.0 18 Fold:Immunoglobulin-like beta-sandwich Superfamily:Purple acid phosphatase, N-terminal domain Family:Purple acid phosphatase, N-terminal domain 45 d2arza1 not modelled 17.4 15 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 46 d2hq9a1 not modelled 16.2 6 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 47 c2ou5B_ not modelled 11.6 12 PDB header:flavoprotein Chain: B: PDB Molecule:pyridoxamine 5'-phosphate oxidase-related, fmn-binding; PDBTitle: crystal structure of a pyridoxamine 5'-phosphate oxidase-related fmn-2 binding protein (jann_0254) from jannaschia sp. ccs1 at 1.60 a3 resolution 48 c2ig6B_ not modelled 9.5 13 PDB header:oxidoreductase Chain: B: PDB Molecule:nimc/nima family protein; PDBTitle: crystal structure of a nimc/nima family protein (ca_c2569) from2 clostridium acetobutylicum at 1.80 a resolution 49 d2qfra1 not modelled 9.2 11 Fold:Immunoglobulin-like beta-sandwich Superfamily:Purple acid phosphatase, N-terminal domain Family:Purple acid phosphatase, N-terminal domain 50 c2iabB_ not modelled 8.1 14 PDB header:unknown function Chain: B: PDB Molecule:hypothetical protein; PDBTitle: crystal structure of a protein with fmn-binding split barrel fold2 (np_828636.1) from streptomyces avermitilis at 2.00 a resolution 51 d2i02a1 not modelled 7.9 13 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 52 d1mkya3 not modelled 7.3 25 Fold:Alpha-lytic protease prodomain-like Superfamily:Probable GTPase Der, C-terminal domain Family:Probable GTPase Der, C-terminal domain 53 c3cp3A_ not modelled 7.3 13 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of conserved protein of unknown function dip18742 from corynebacterium diphtheriae 54 c1a2oB_ not modelled 7.1 17 PDB header:bacterial chemotaxis Chain: B: PDB Molecule:cheb methylesterase; PDBTitle: structural basis for methylesterase cheb regulation by a2 phosphorylation-activated domain 55 d1dmla2 not modelled 7.0 24 Fold:DNA clamp Superfamily:DNA clamp Family:DNA polymerase processivity factor 56 d2fg9a1 not modelled 6.3 15 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 57 c2hhzA_ not modelled 6.0 6 PDB header:oxidoreductase Chain: A: PDB Molecule:pyridoxamine 5'-phosphate oxidase-related; PDBTitle: crystal structure of a pyridoxamine 5'-phosphate oxidase-related2 protein (ssuidraft_2804) from streptococcus suis 89/1591 at 2.00 a3 resolution 58 d1svba1 not modelled 5.9 13 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:Class II viral fusion proteins C-terminal domain 59 c1mkyA_ not modelled 5.4 20 PDB header:ligand binding protein Chain: A: PDB Molecule:probable gtp-binding protein enga; PDBTitle: structural analysis of the domain interactions in der, a2 switch protein containing two gtpase domains

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0