Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	R	R	A	I	G	V	S	E	R	P	P	L	L	Q	T	I	P	L	S	L	Q	H	L	F	A	M	F	G	A	T	V	L	V	P	V	L	F	H	I	N	P	A	T	V	L	L	F	N	G	I	G	T	L	L	Y	L	F	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		C	K	G	K	I	P	A	Y	L	G	S	S	F	A	F	I	S	P	V	L	L	L	L	P	L	G	Y	E	V	A	L	G	G	F	I	M	C	G	V	L	F	C	L	V	S	F	I	V	K	K	A	G	T	G	W	L	D	V	L	F
Secondary structure
SS confidence
Disorder																								?		?																										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		P	P	A	A	M	G	A	I	V	A	V	I	G	L	E	L	A	G	V	A	A	G	M	A	G	L	L	P	A	E	G	Q	T	P	D	S	K	T	I	I	I	S	I	T	T	L	A	V	T	V	L	G	S	V	L	F	R	G	F	L
Secondary structure
SS confidence
Disorder																																																										?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	I	I	P	I	L	I	G	V	L	V	G	Y	A	L	S	F	A	M	G	I	V	D	T	T	P	I	I	N	A	H	W	F	A	L	P	T	L	Y	T	P	R	F	E	W	F	A	I	L	T	I	L	P	A	A	L	V	V	I	A
Secondary structure
SS confidence
Disorder		?	?																									?		?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		E	H	V	G	H	L	V	V	T	A	N	I	V	K	K	D	L	L	R	D	P	G	L	H	R	S	M	F	A	N	G	L	S	T	V	I	S	G	F	F	G	S	T	P	N	T	T	Y	G	E	N	I	G	V	M	A	I	T	R	V
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?	?	?	?	?																			?		?	?	?	?	?	?										?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		Y	S	T	W	V	I	G	G	A	A	I	F	A	I	L	L	S	C	V	G	K	L	A	A	A	I	Q	M	I	P	L	P	V	M	G	G	V	S	L	L	L	Y	G	V	I	G	A	S	G	I	R	V	L	I	E	S	K	V	D	Y
Secondary structure
SS confidence
Disorder		?	?																																																					?		?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		N	K	A	Q	N	L	I	L	T	S	V	I	L	I	I	G	V	S	G	A	K	V	N	I	G	A	A	E	L	K	G	M	A	L	A	T	I	V	G	I	G	L	S	L	I	F	K	L	I	S	V	L	R	P	E	E	V	V	L	D
Secondary structure
SS confidence
Disorder																													?	?																		?		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.
Sequence		A	E	D	A	D	I	T	D	K
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3qe7 chain A Region: 4 - 408 Aligned: 405 Modelled: 405 Confidence: 100.0% Identity: 100% PDB header:transport protein Chain: A: PDB Molecule:uracil permease; PDBTitle: crystal structure of uracil transporter--uraa Phyre2 PDB 1dxz chain A Region: 10 - 33 Aligned: 24 Modelled: 24 Confidence: 27.8% Identity: 21% PDB header:transmembrane protein Chain: A: PDB Molecule:acetylcholine receptor protein, alpha chain; PDBTitle: m2 transmembrane segment of alpha-subunit of nicotinic2 acetylcholine receptor from torpedo californica, nmr, 203 structures Phyre2 PDB 3lpz chain A Region: 271 - 287 Aligned: 17 Modelled: 17 Confidence: 19.6% Identity: 18% PDB header:protein transport Chain: A: PDB Molecule:get4 (yor164c homolog); PDBTitle: crystal structure of c. therm. get4 Phyre2 PDB 1v53 chain A domain 1 Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 9.7% Identity: 19% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 1x0l chain B Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 8.9% Identity: 14% PDB header:oxidoreductase Chain: B: PDB Molecule:homoisocitrate dehydrogenase; PDBTitle: crystal structure of tetrameric homoisocitrate dehydrogenase from an2 extreme thermophile, thermus thermophilus Phyre2 PDB 1g2u chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 8.7% Identity: 19% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 1xac chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 7.9% Identity: 19% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 3u1h chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 7.7% Identity: 19% PDB header:oxidoreductase Chain: A: PDB Molecule:3-isopropylmalate dehydrogenase; PDBTitle: crystal structure of ipmdh from the last common ancestor of bacillus Phyre2 PDB 3fmx chain X Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 7.5% Identity: 17% PDB header:oxidoreductase Chain: X: PDB Molecule:tartrate dehydrogenase/decarboxylase; PDBTitle: crystal structure of tartrate dehydrogenase from pseudomonas2 putida complexed with nadh Phyre2 PDB 1cnz chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 7.5% Identity: 21% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 3rj1 chain R Region: 405 - 429 Aligned: 25 Modelled: 25 Confidence: 6.9% Identity: 8% PDB header:transcription Chain: R: PDB Molecule:mediator of rna polymerase ii transcription subunit 22; PDBTitle: architecture of the mediator head module Phyre2 PDB 1vlc chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 6.8% Identity: 21% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 2d4v chain D Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 6.7% Identity: 7% PDB header:oxidoreductase Chain: D: PDB Molecule:isocitrate dehydrogenase; PDBTitle: crystal structure of nad dependent isocitrate dehydrogenase2 from acidithiobacillus thiooxidans Phyre2 PDB 2jln chain A Region: 72 - 426 Aligned: 353 Modelled: 355 Confidence: 6.5% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter Phyre2 PDB 1cm7 chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 6.5% Identity: 21% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 1wpw chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 6.5% Identity: 21% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 1w0d chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 6.5% Identity: 10% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 1hqs chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 6.4% Identity: 10% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 1a05 chain A Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 6.0% Identity: 21% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 2d1c chain B Region: 247 - 288 Aligned: 42 Modelled: 42 Confidence: 5.9% Identity: 19% PDB header:oxidoreductase Chain: B: PDB Molecule:isocitrate dehydrogenase; PDBTitle: crystal structure of tt0538 protein from thermus thermophilus hb8 Phyre2 1 c3qe7A_ 2 c1dxzA_ 3 c3lpzA_ 4 d1v53a1 5 c1x0lB_ 6 d1g2ua_ 7 d1xaca_ 8 c3u1hA_ 9 c3fmxX_ 10 d1cnza_ 11 c3rj1R_ 12 d1vlca_ 13 c2d4vD_ 14 c2jlnA_ 15 d1cm7a_ 16 d1wpwa_ 17 d1w0da_ 18 d1hqsa_ 19 d1a05a_ 20 c2d1cB_ 21 22

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3qe7A_ 100.0 100 PDB header:transport protein Chain: A: PDB Molecule:uracil permease; PDBTitle: crystal structure of uracil transporter--uraa 2 c1dxzA_ 27.8 21 PDB header:transmembrane protein Chain: A: PDB Molecule:acetylcholine receptor protein, alpha chain; PDBTitle: m2 transmembrane segment of alpha-subunit of nicotinic2 acetylcholine receptor from torpedo californica, nmr, 203 structures 3 c3lpzA_ 19.6 18 PDB header:protein transport Chain: A: PDB Molecule:get4 (yor164c homolog); PDBTitle: crystal structure of c. therm. get4 4 d1v53a1 9.7 19 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 5 c1x0lB_ 8.9 14 PDB header:oxidoreductase Chain: B: PDB Molecule:homoisocitrate dehydrogenase; PDBTitle: crystal structure of tetrameric homoisocitrate dehydrogenase from an2 extreme thermophile, thermus thermophilus 6 d1g2ua_ 8.7 19 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 7 d1xaca_ 7.9 19 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 8 c3u1hA_ 7.7 19 PDB header:oxidoreductase Chain: A: PDB Molecule:3-isopropylmalate dehydrogenase; PDBTitle: crystal structure of ipmdh from the last common ancestor of bacillus 9 c3fmxX_ 7.5 17 PDB header:oxidoreductase Chain: X: PDB Molecule:tartrate dehydrogenase/decarboxylase; PDBTitle: crystal structure of tartrate dehydrogenase from pseudomonas2 putida complexed with nadh 10 d1cnza_ 7.5 21 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 11 c3rj1R_ 6.9 8 PDB header:transcription Chain: R: PDB Molecule:mediator of rna polymerase ii transcription subunit 22; PDBTitle: architecture of the mediator head module 12 d1vlca_ 6.8 21 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 13 c2d4vD_ 6.7 7 PDB header:oxidoreductase Chain: D: PDB Molecule:isocitrate dehydrogenase; PDBTitle: crystal structure of nad dependent isocitrate dehydrogenase2 from acidithiobacillus thiooxidans 14 c2jlnA_ 6.5 11 PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter 15 d1cm7a_ 6.5 21 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 16 d1wpwa_ 6.5 21 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 17 d1w0da_ 6.5 10 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 18 d1hqsa_ 6.4 10 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 19 d1a05a_ 6.0 21 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 20 c2d1cB_ 5.9 19 PDB header:oxidoreductase Chain: B: PDB Molecule:isocitrate dehydrogenase; PDBTitle: crystal structure of tt0538 protein from thermus thermophilus hb8 21 c3r8wC_ not modelled 5.6 19 PDB header:oxidoreductase Chain: C: PDB Molecule:3-isopropylmalate dehydrogenase 2, chloroplastic; PDBTitle: structure of 3-isopropylmalate dehydrogenase isoform 2 from2 arabidopsis thaliana at 2.2 angstrom resolution 22 d1pb1a_ not modelled 5.6 10 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0