Secondary structure and disorder prediction

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Sequence		M	I	T	N	Y	E	A	T	V	V	T	T	D	D	I	V	H	E	V	N	L	E	G	K	R	I	G	Y	V	I	K	T	E	N	K	E	T	P	F	T	V	V	D	I	D	G	P	S	G	N	V	K	T	L	D	E	G	V	K	K
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.
Sequence		M	C	L	V	H	I	G	K	N	L	P	A	E	K	K	A	E	F	L	A	T	L	I	A	M	K	L	K	G	E	I
Secondary structure
SS confidence
Disorder																										?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2o14 chain A domain 1 Region: 4 - 46 Aligned: 43 Modelled: 43 Confidence: 31.3% Identity: 23% Fold: Galactose-binding domain-like Superfamily: Galactose-binding domain-like Family: YxiM N-terminal domain-like Phyre2 PDB 2ges chain A Region: 34 - 53 Aligned: 19 Modelled: 20 Confidence: 25.6% Identity: 21% PDB header:transferase Chain: A: PDB Molecule:pantothenate kinase; PDBTitle: pantothenate kinase from mycobacterium tuberculosis (mtpank) in2 complex with a coenzyme a derivative, form-i (rt) Phyre2 PDB 1sq5 chain A Region: 33 - 53 Aligned: 20 Modelled: 21 Confidence: 25.5% Identity: 25% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: Phosphoribulokinase/pantothenate kinase Phyre2 PDB 2qkd chain A Region: 34 - 50 Aligned: 16 Modelled: 17 Confidence: 20.1% Identity: 63% PDB header:signaling protein, cell cycle Chain: A: PDB Molecule:zinc finger protein zpr1; PDBTitle: crystal structure of tandem zpr1 domains Phyre2 PDB 1rz3 chain A Region: 35 - 53 Aligned: 19 Modelled: 19 Confidence: 16.2% Identity: 42% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: Phosphoribulokinase/pantothenate kinase Phyre2 PDB 3b7x chain A Region: 25 - 62 Aligned: 38 Modelled: 38 Confidence: 15.5% Identity: 16% PDB header:isomerase Chain: A: PDB Molecule:fk506-binding protein 6; PDBTitle: crystal structure of human fk506-binding protein 6 Phyre2 PDB 1odf chain A Region: 31 - 53 Aligned: 22 Modelled: 23 Confidence: 14.3% Identity: 23% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: Phosphoribulokinase/pantothenate kinase Phyre2 PDB 2cp6 chain A domain 1 Region: 19 - 50 Aligned: 32 Modelled: 32 Confidence: 12.7% Identity: 25% Fold: SH3-like barrel Superfamily: Cap-Gly domain Family: Cap-Gly domain Phyre2 PDB 1nij chain A domain 2 Region: 63 - 82 Aligned: 20 Modelled: 20 Confidence: 12.2% Identity: 40% Fold: Hypothetical protein YjiA, C-terminal domain Superfamily: Hypothetical protein YjiA, C-terminal domain Family: Hypothetical protein YjiA, C-terminal domain Phyre2 PDB 3tqc chain B Region: 30 - 53 Aligned: 23 Modelled: 24 Confidence: 11.8% Identity: 17% PDB header:transferase Chain: B: PDB Molecule:pantothenate kinase; PDBTitle: structure of the pantothenate kinase (coaa) from coxiella burnetii Phyre2 PDB 3asy chain B Region: 38 - 53 Aligned: 15 Modelled: 16 Confidence: 11.2% Identity: 47% PDB header:transferase Chain: B: PDB Molecule:uridine kinase; PDBTitle: ligand-free structure of uridine kinase from thermus thermophilus hb8 Phyre2 PDB 1umg chain A Region: 45 - 61 Aligned: 17 Modelled: 17 Confidence: 10.8% Identity: 53% Fold: Sulfolobus fructose-1,6-bisphosphatase-like Superfamily: Sulfolobus fructose-1,6-bisphosphatase-like Family: Sulfolobus fructose-1,6-bisphosphatase-like Phyre2 PDB 3iz5 chain J Region: 23 - 51 Aligned: 29 Modelled: 29 Confidence: 9.0% Identity: 45% PDB header:ribosome Chain: J: PDB Molecule:60s ribosomal protein l12 (l11p); PDBTitle: localization of the large subunit ribosomal proteins into a 5.5 a2 cryo-em map of triticum aestivum translating 80s ribosome Phyre2 PDB 2f1l chain A domain 1 Region: 12 - 26 Aligned: 15 Modelled: 14 Confidence: 8.6% Identity: 20% Fold: PRC-barrel domain Superfamily: PRC-barrel domain Family: RimM C-terminal domain-like Phyre2 PDB 1vm6 chain A domain 2 Region: 44 - 60 Aligned: 17 Modelled: 17 Confidence: 7.7% Identity: 35% Fold: FwdE/GAPDH domain-like Superfamily: Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family: Dihydrodipicolinate reductase-like Phyre2 PDB 1xmk chain A domain 1 Region: 64 - 91 Aligned: 28 Modelled: 28 Confidence: 7.1% Identity: 29% Fold: DNA/RNA-binding 3-helical bundle Superfamily: "Winged helix" DNA-binding domain Family: Z-DNA binding domain Phyre2 PDB 1tj1 chain A domain 1 Region: 55 - 63 Aligned: 9 Modelled: 9 Confidence: 6.9% Identity: 56% Fold: N-terminal domain of bifunctional PutA protein Superfamily: N-terminal domain of bifunctional PutA protein Family: N-terminal domain of bifunctional PutA protein Phyre2 PDB 2bps chain A Region: 25 - 40 Aligned: 16 Modelled: 16 Confidence: 6.3% Identity: 38% PDB header:ubiquitin Chain: A: PDB Molecule:yukd protein; PDBTitle: ubiquitin-like protein yukd of bacillus subtilis Phyre2 PDB 2ajm chain A Region: 52 - 63 Aligned: 12 Modelled: 12 Confidence: 6.1% Identity: 42% PDB header:membrane protein Chain: A: PDB Molecule:nonstructural protein 5a; PDBTitle: nmr structure of the in-plane membrane anchor domain [1-28]2 of the monotopic nonstructural protein 5a (ns5a) from the3 bovine viral diarrhea virus (bvdv) Phyre2 PDB 2oo3 chain A domain 1 Region: 34 - 49 Aligned: 16 Modelled: 16 Confidence: 6.0% Identity: 19% Fold: S-adenosyl-L-methionine-dependent methyltransferases Superfamily: S-adenosyl-L-methionine-dependent methyltransferases Family: LPG1296-like Phyre2 1 d2o14a1 2 c2gesA_ 3 d1sq5a_ 4 c2qkdA_ 5 d1rz3a_ 6 c3b7xA_ 7 d1odfa_ 8 d2cp6a1 9 d1nija2 10 c3tqcB_ 11 c3asyB_ 12 d1umga_ 13 c3iz5j_ 14 d2f1la1 15 d1vm6a2 16 d1xmka1 17 d1tj1a1 18 c2bpsA_ 19 c2ajmA_ 20 d2oo3a1 21 22 23 24 25 26 27 28

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2o14a1 31.3 23 Fold:Galactose-binding domain-like Superfamily:Galactose-binding domain-like Family:YxiM N-terminal domain-like 2 c2gesA_ 25.6 21 PDB header:transferase Chain: A: PDB Molecule:pantothenate kinase; PDBTitle: pantothenate kinase from mycobacterium tuberculosis (mtpank) in2 complex with a coenzyme a derivative, form-i (rt) 3 d1sq5a_ 25.5 25 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Phosphoribulokinase/pantothenate kinase 4 c2qkdA_ 20.1 63 PDB header:signaling protein, cell cycle Chain: A: PDB Molecule:zinc finger protein zpr1; PDBTitle: crystal structure of tandem zpr1 domains 5 d1rz3a_ 16.2 42 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Phosphoribulokinase/pantothenate kinase 6 c3b7xA_ 15.5 16 PDB header:isomerase Chain: A: PDB Molecule:fk506-binding protein 6; PDBTitle: crystal structure of human fk506-binding protein 6 7 d1odfa_ 14.3 23 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Phosphoribulokinase/pantothenate kinase 8 d2cp6a1 12.7 25 Fold:SH3-like barrel Superfamily:Cap-Gly domain Family:Cap-Gly domain 9 d1nija2 12.2 40 Fold:Hypothetical protein YjiA, C-terminal domain Superfamily:Hypothetical protein YjiA, C-terminal domain Family:Hypothetical protein YjiA, C-terminal domain 10 c3tqcB_ 11.8 17 PDB header:transferase Chain: B: PDB Molecule:pantothenate kinase; PDBTitle: structure of the pantothenate kinase (coaa) from coxiella burnetii 11 c3asyB_ 11.2 47 PDB header:transferase Chain: B: PDB Molecule:uridine kinase; PDBTitle: ligand-free structure of uridine kinase from thermus thermophilus hb8 12 d1umga_ 10.8 53 Fold:Sulfolobus fructose-1,6-bisphosphatase-like Superfamily:Sulfolobus fructose-1,6-bisphosphatase-like Family:Sulfolobus fructose-1,6-bisphosphatase-like 13 c3iz5j_ 9.0 45 PDB header:ribosome Chain: J: PDB Molecule:60s ribosomal protein l12 (l11p); PDBTitle: localization of the large subunit ribosomal proteins into a 5.5 a2 cryo-em map of triticum aestivum translating 80s ribosome 14 d2f1la1 8.6 20 Fold:PRC-barrel domain Superfamily:PRC-barrel domain Family:RimM C-terminal domain-like 15 d1vm6a2 7.7 35 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:Dihydrodipicolinate reductase-like 16 d1xmka1 7.1 29 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:Z-DNA binding domain 17 d1tj1a1 6.9 56 Fold:N-terminal domain of bifunctional PutA protein Superfamily:N-terminal domain of bifunctional PutA protein Family:N-terminal domain of bifunctional PutA protein 18 c2bpsA_ 6.3 38 PDB header:ubiquitin Chain: A: PDB Molecule:yukd protein; PDBTitle: ubiquitin-like protein yukd of bacillus subtilis 19 c2ajmA_ 6.1 42 PDB header:membrane protein Chain: A: PDB Molecule:nonstructural protein 5a; PDBTitle: nmr structure of the in-plane membrane anchor domain [1-28]2 of the monotopic nonstructural protein 5a (ns5a) from the3 bovine viral diarrhea virus (bvdv) 20 d2oo3a1 6.0 19 Fold:S-adenosyl-L-methionine-dependent methyltransferases Superfamily:S-adenosyl-L-methionine-dependent methyltransferases Family:LPG1296-like 21 c1h8bB_ not modelled 5.8 64 PDB header:structural protein Chain: B: PDB Molecule:titin; PDBTitle: ef-hands 3,4 from alpha-actinin / z-repeat 7 from titin 22 d1yl7a2 not modelled 5.6 24 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:Dihydrodipicolinate reductase-like 23 d1iqca2 not modelled 5.5 21 Fold:Cytochrome c Superfamily:Cytochrome c Family:Di-heme cytochrome c peroxidase 24 c3bguA_ not modelled 5.5 10 PDB header:unknown function Chain: A: PDB Molecule:ferredoxin-like protein of unknown function; PDBTitle: crystal structure of a dimeric ferredoxin-like protein of unknown2 function (tfu_0763) from thermobifida fusca yx at 1.50 a resolution 25 d2b1ya1 not modelled 5.4 36 Fold:Atu1913-like Superfamily:Atu1913-like Family:Atu1913-like 26 d1ou8a_ not modelled 5.4 42 Fold:SspB-like Superfamily:SspB-like Family:Stringent starvation protein B, SspB 27 c2wwiC_ not modelled 5.2 20 PDB header:transferase Chain: C: PDB Molecule:thymidilate kinase, putative; PDBTitle: plasmodium falciparum thymidylate kinase in complex with2 aztmp and adp 28 c3c8uA_ not modelled 5.1 31 PDB header:transferase Chain: A: PDB Molecule:fructokinase; PDBTitle: crystal structure of putative fructose transport system kinase2 (yp_612366.1) from silicibacter sp. tm1040 at 1.95 a resolution

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0