Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	G	M	T	N	V	T	K	L	A	S	E	P	S	G	Q	E	F	L	V	F	T	L	G	D	E	E	Y	G	I	D	I	L	K	V	Q	E	I	R	G	Y	D	Q	V	T	R	I	A	N	T	P	A	F	I	K	G	V	T	N
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?																												?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	R	G	V	I	V	P	I	V	D	L	R	I	K	F	S	Q	V	D	V	D	Y	N	D	N	T	V	V	I	V	L	N	L	G	Q	R	V	V	G	I	V	V	D	G	V	S	D	V	L	S	L	T	A	E	Q	I	R	P	A	P
Secondary structure
SS confidence
Disorder																			?	?	?	?	?	?																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.
Sequence		E	F	A	V	T	L	S	T	E	Y	L	T	G	L	G	A	L	G	D	R	M	L	I	L	V	N	I	E	K	L	L	N	S	E	E	M	A	L	L	D	S	A	A	S	E	V	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																																						?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2ho9 chain A Region: 1 - 167 Aligned: 167 Modelled: 167 Confidence: 100.0% Identity: 100% PDB header:signaling protein Chain: A: PDB Molecule:chemotaxis protein chew; PDBTitle: solution structure of chemotaxi protein chew from2 escherichia coli Phyre2 PDB 2qdl chain B Region: 16 - 155 Aligned: 139 Modelled: 140 Confidence: 100.0% Identity: 29% PDB header:signaling protein Chain: B: PDB Molecule:chemotaxis signal transduction protein; PDBTitle: crystal structure of scaffolding protein ttchew from2 thermoanaerobacter tengcongensis Phyre2 PDB 2ch4 chain W domain 1 Region: 16 - 153 Aligned: 135 Modelled: 138 Confidence: 100.0% Identity: 24% Fold: OB-fold Superfamily: CheW-like Family: CheW-like Phyre2 PDB 1b3q chain A domain 2 Region: 18 - 151 Aligned: 127 Modelled: 134 Confidence: 99.9% Identity: 17% Fold: OB-fold Superfamily: CheW-like Family: CheW-like Phyre2 PDB 1b3q chain A Region: 10 - 151 Aligned: 132 Modelled: 142 Confidence: 99.9% Identity: 17% PDB header:transferase Chain: A: PDB Molecule:protein (chemotaxis protein chea); PDBTitle: crystal structure of chea-289, a signal transducing histidine kinase Phyre2 PDB 2ch4 chain A Region: 3 - 151 Aligned: 139 Modelled: 149 Confidence: 99.7% Identity: 17% PDB header:transferase/chemotaxis Chain: A: PDB Molecule:chemotaxis protein chea; PDBTitle: complex between bacterial chemotaxis histidine kinase chea2 domains p4 and p5 and receptor-adaptor protein chew Phyre2 PDB 2kk4 chain A Region: 19 - 39 Aligned: 21 Modelled: 21 Confidence: 20.8% Identity: 14% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein af_2094; PDBTitle: solution nmr structure of protein af2094 from archaeoglobus2 fulgidus. northeast structural genomics consotium (nesg)3 target gt2 Phyre2 PDB 2xd0 chain B Region: 20 - 70 Aligned: 50 Modelled: 51 Confidence: 11.9% Identity: 16% PDB header:toxin/rna Chain: B: PDB Molecule:toxn; PDBTitle: a processed non-coding rna regulates a bacterial antiviral2 system Phyre2 PDB 2p12 chain A domain 1 Region: 129 - 167 Aligned: 39 Modelled: 39 Confidence: 8.4% Identity: 26% Fold: FomD barrel-like Superfamily: FomD-like Family: FomD-like Phyre2 1 c2ho9A_ 2 c2qdlB_ 3 d2ch4w1 4 d1b3qa2 5 c1b3qA_ 6 c2ch4A_ 7 c2kk4A_ 8 c2xd0B_ 9 d2p12a1

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2ho9A_ 100.0 100 PDB header:signaling protein Chain: A: PDB Molecule:chemotaxis protein chew; PDBTitle: solution structure of chemotaxi protein chew from2 escherichia coli 2 c2qdlB_ 100.0 29 PDB header:signaling protein Chain: B: PDB Molecule:chemotaxis signal transduction protein; PDBTitle: crystal structure of scaffolding protein ttchew from2 thermoanaerobacter tengcongensis 3 d2ch4w1 100.0 24 Fold:OB-fold Superfamily:CheW-like Family:CheW-like 4 d1b3qa2 99.9 17 Fold:OB-fold Superfamily:CheW-like Family:CheW-like 5 c1b3qA_ 99.9 17 PDB header:transferase Chain: A: PDB Molecule:protein (chemotaxis protein chea); PDBTitle: crystal structure of chea-289, a signal transducing histidine kinase 6 c2ch4A_ 99.7 17 PDB header:transferase/chemotaxis Chain: A: PDB Molecule:chemotaxis protein chea; PDBTitle: complex between bacterial chemotaxis histidine kinase chea2 domains p4 and p5 and receptor-adaptor protein chew 7 c2kk4A_ 20.8 14 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein af_2094; PDBTitle: solution nmr structure of protein af2094 from archaeoglobus2 fulgidus. northeast structural genomics consotium (nesg)3 target gt2 8 c2xd0B_ 11.9 16 PDB header:toxin/rna Chain: B: PDB Molecule:toxn; PDBTitle: a processed non-coding rna regulates a bacterial antiviral2 system 9 d2p12a1 8.4 26 Fold:FomD barrel-like Superfamily:FomD-like Family:FomD-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0