Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	Q	D	Y	E	L	V	V	K	G	V	R	N	F	E	N	K	V	T	V	T	V	A	L	Q	D	K	E	R	F	D	G	E	I	F	D	L	D	V	A	M	D	R	V	E	G	A	A	L	E	F	Y	E	A	A	A	R	R	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?																					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.
Sequence		V	R	Q	V	F	L	E	V	A	E	K	L	S	E	K	V	E	S	Y	L	Q	H	Q	Y	S	F	K	I	E	N	P	A	N	K	H	E	R	P	H	H	K	Y	L
Secondary structure
SS confidence
Disorder															?	?	?	?	?	?	?	?	?									?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1jkx chain A Region: 12 - 86 Aligned: 74 Modelled: 75 Confidence: 27.5% Identity: 9% Fold: Formyltransferase Superfamily: Formyltransferase Family: Formyltransferase Phyre2 PDB 2een chain A Region: 37 - 84 Aligned: 45 Modelled: 48 Confidence: 25.4% Identity: 22% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein ph1819; PDBTitle: structure of ph1819 protein from pyrococcus horikoshii ot3 Phyre2 PDB 1fmt chain A domain 2 Region: 12 - 81 Aligned: 69 Modelled: 70 Confidence: 21.8% Identity: 10% Fold: Formyltransferase Superfamily: Formyltransferase Family: Formyltransferase Phyre2 PDB 2zfd chain B Region: 38 - 65 Aligned: 28 Modelled: 28 Confidence: 17.2% Identity: 11% PDB header:signaling protein/transferase Chain: B: PDB Molecule:putative uncharacterized protein t20l15_90; PDBTitle: the crystal structure of plant specific calcium binding protein atcbl22 in complex with the regulatory domain of atcipk14 Phyre2 PDB 2bw0 chain A domain 2 Region: 12 - 82 Aligned: 71 Modelled: 71 Confidence: 17.2% Identity: 8% Fold: Formyltransferase Superfamily: Formyltransferase Family: Formyltransferase Phyre2 PDB 2v4o chain B Region: 1 - 91 Aligned: 77 Modelled: 91 Confidence: 17.0% Identity: 21% PDB header:hydrolase Chain: B: PDB Molecule:multifunctional protein sur e; PDBTitle: crystal structure of salmonella typhimurium sure at 2.752 angstrom resolution in monoclinic form Phyre2 PDB 3oqu chain B Region: 1 - 76 Aligned: 72 Modelled: 76 Confidence: 17.0% Identity: 19% PDB header:hormone receptor Chain: B: PDB Molecule:abscisic acid receptor pyl9; PDBTitle: crystal structure of native abscisic acid receptor pyl9 with aba Phyre2 PDB 1wjw chain A Region: 51 - 81 Aligned: 31 Modelled: 31 Confidence: 16.4% Identity: 10% Fold: TBP-like Superfamily: Phosphoglucomutase, C-terminal domain Family: Phosphoglucomutase, C-terminal domain Phyre2 PDB 2bln chain A domain 2 Region: 12 - 81 Aligned: 69 Modelled: 70 Confidence: 15.2% Identity: 14% Fold: Formyltransferase Superfamily: Formyltransferase Family: Formyltransferase Phyre2 PDB 2ae8 chain A domain 2 Region: 21 - 54 Aligned: 33 Modelled: 34 Confidence: 12.7% Identity: 18% Fold: Ribosomal protein S5 domain 2-like Superfamily: Ribosomal protein S5 domain 2-like Family: Imidazole glycerol phosphate dehydratase Phyre2 PDB 1civ chain A domain 2 Region: 12 - 82 Aligned: 71 Modelled: 71 Confidence: 11.8% Identity: 10% Fold: LDH C-terminal domain-like Superfamily: LDH C-terminal domain-like Family: Lactate & malate dehydrogenases, C-terminal domain Phyre2 PDB 2fjt chain A Region: 21 - 84 Aligned: 63 Modelled: 64 Confidence: 11.4% Identity: 17% PDB header:lyase Chain: A: PDB Molecule:adenylyl cyclase class iv; PDBTitle: adenylyl cyclase class iv from yersinia pestis Phyre2 PDB 1xo8 chain A Region: 7 - 50 Aligned: 43 Modelled: 44 Confidence: 11.3% Identity: 12% Fold: Immunoglobulin-like beta-sandwich Superfamily: LEA14-like Family: LEA14-like Phyre2 PDB 3rfo chain A Region: 12 - 81 Aligned: 69 Modelled: 67 Confidence: 10.7% Identity: 13% PDB header:transferase Chain: A: PDB Molecule:methionyl-trna formyltransferase; PDBTitle: crystal structure of methyionyl-trna formyltransferase from bacillus2 anthracis Phyre2 PDB 3p9x chain B Region: 12 - 79 Aligned: 67 Modelled: 68 Confidence: 9.9% Identity: 21% PDB header:transferase Chain: B: PDB Molecule:phosphoribosylglycinamide formyltransferase; PDBTitle: crystal structure of phosphoribosylglycinamide formyltransferase from2 bacillus halodurans Phyre2 PDB 2ldx chain A domain 2 Region: 12 - 72 Aligned: 61 Modelled: 61 Confidence: 9.3% Identity: 8% Fold: LDH C-terminal domain-like Superfamily: LDH C-terminal domain-like Family: Lactate & malate dehydrogenases, C-terminal domain Phyre2 PDB 1ev0 chain A Region: 10 - 35 Aligned: 26 Modelled: 26 Confidence: 8.8% Identity: 27% Fold: Cell division protein MinE topological specificity domain Superfamily: Cell division protein MinE topological specificity domain Family: Cell division protein MinE topological specificity domain Phyre2 PDB 2f1d chain A domain 2 Region: 21 - 54 Aligned: 33 Modelled: 34 Confidence: 8.6% Identity: 21% Fold: Ribosomal protein S5 domain 2-like Superfamily: Ribosomal protein S5 domain 2-like Family: Imidazole glycerol phosphate dehydratase Phyre2 PDB 1s3i chain A domain 2 Region: 12 - 81 Aligned: 70 Modelled: 70 Confidence: 8.6% Identity: 10% Fold: Formyltransferase Superfamily: Formyltransferase Family: Formyltransferase Phyre2 PDB 1ybx chain A Region: 16 - 72 Aligned: 51 Modelled: 57 Confidence: 8.5% Identity: 20% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved hypothetical protein; PDBTitle: conserved hypothetical protein cth-383 from clostridium thermocellum Phyre2 1 d1jkxa_ 2 c2eenA_ 3 d1fmta2 4 c2zfdB_ 5 d2bw0a2 6 c2v4oB_ 7 c3oquB_ 8 d1wjwa_ 9 d2blna2 10 d2ae8a2 11 d1civa2 12 c2fjtA_ 13 d1xo8a_ 14 c3rfoA_ 15 c3p9xB_ 16 d2ldxa2 17 d1ev0a_ 18 d2f1da2 19 d1s3ia2 20 c1ybxA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1jkxa_ 27.5 9 Fold:Formyltransferase Superfamily:Formyltransferase Family:Formyltransferase 2 c2eenA_ 25.4 22 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein ph1819; PDBTitle: structure of ph1819 protein from pyrococcus horikoshii ot3 3 d1fmta2 21.8 10 Fold:Formyltransferase Superfamily:Formyltransferase Family:Formyltransferase 4 c2zfdB_ 17.2 11 PDB header:signaling protein/transferase Chain: B: PDB Molecule:putative uncharacterized protein t20l15_90; PDBTitle: the crystal structure of plant specific calcium binding protein atcbl22 in complex with the regulatory domain of atcipk14 5 d2bw0a2 17.2 8 Fold:Formyltransferase Superfamily:Formyltransferase Family:Formyltransferase 6 c2v4oB_ 17.0 21 PDB header:hydrolase Chain: B: PDB Molecule:multifunctional protein sur e; PDBTitle: crystal structure of salmonella typhimurium sure at 2.752 angstrom resolution in monoclinic form 7 c3oquB_ 17.0 19 PDB header:hormone receptor Chain: B: PDB Molecule:abscisic acid receptor pyl9; PDBTitle: crystal structure of native abscisic acid receptor pyl9 with aba 8 d1wjwa_ 16.4 10 Fold:TBP-like Superfamily:Phosphoglucomutase, C-terminal domain Family:Phosphoglucomutase, C-terminal domain 9 d2blna2 15.2 14 Fold:Formyltransferase Superfamily:Formyltransferase Family:Formyltransferase 10 d2ae8a2 12.7 18 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:Imidazole glycerol phosphate dehydratase 11 d1civa2 11.8 10 Fold:LDH C-terminal domain-like Superfamily:LDH C-terminal domain-like Family:Lactate & malate dehydrogenases, C-terminal domain 12 c2fjtA_ 11.4 17 PDB header:lyase Chain: A: PDB Molecule:adenylyl cyclase class iv; PDBTitle: adenylyl cyclase class iv from yersinia pestis 13 d1xo8a_ 11.3 12 Fold:Immunoglobulin-like beta-sandwich Superfamily:LEA14-like Family:LEA14-like 14 c3rfoA_ 10.7 13 PDB header:transferase Chain: A: PDB Molecule:methionyl-trna formyltransferase; PDBTitle: crystal structure of methyionyl-trna formyltransferase from bacillus2 anthracis 15 c3p9xB_ 9.9 21 PDB header:transferase Chain: B: PDB Molecule:phosphoribosylglycinamide formyltransferase; PDBTitle: crystal structure of phosphoribosylglycinamide formyltransferase from2 bacillus halodurans 16 d2ldxa2 9.3 8 Fold:LDH C-terminal domain-like Superfamily:LDH C-terminal domain-like Family:Lactate & malate dehydrogenases, C-terminal domain 17 d1ev0a_ 8.8 27 Fold:Cell division protein MinE topological specificity domain Superfamily:Cell division protein MinE topological specificity domain Family:Cell division protein MinE topological specificity domain 18 d2f1da2 8.6 21 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:Imidazole glycerol phosphate dehydratase 19 d1s3ia2 8.6 10 Fold:Formyltransferase Superfamily:Formyltransferase Family:Formyltransferase 20 c1ybxA_ 8.5 20 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved hypothetical protein; PDBTitle: conserved hypothetical protein cth-383 from clostridium thermocellum 21 c2y94C_ not modelled 8.4 36 PDB header:transferase Chain: C: PDB Molecule:5'-amp-activated protein kinase catalytic subunit alpha-1; PDBTitle: structure of an active form of mammalian ampk 22 d1gsoa1 not modelled 8.1 6 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 23 c2f1dP_ not modelled 7.6 21 PDB header:lyase Chain: P: PDB Molecule:imidazoleglycerol-phosphate dehydratase 1; PDBTitle: x-ray structure of imidazoleglycerol-phosphate dehydratase 24 c3n5fB_ not modelled 7.5 12 PDB header:hydrolase Chain: B: PDB Molecule:n-carbamoyl-l-amino acid hydrolase; PDBTitle: crystal structure of l-n-carbamoylase from geobacillus2 stearothermophilus cect43 25 d1rhya2 not modelled 7.4 11 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:Imidazole glycerol phosphate dehydratase 26 c1rhyB_ not modelled 7.3 12 PDB header:lyase Chain: B: PDB Molecule:imidazole glycerol phosphate dehydratase; PDBTitle: crystal structure of imidazole glycerol phosphate dehydratase 27 c2ae8C_ not modelled 7.2 19 PDB header:lyase Chain: C: PDB Molecule:imidazoleglycerol-phosphate dehydratase; PDBTitle: crystal structure of imidazoleglycerol-phosphate dehydratase from2 staphylococcus aureus subsp. aureus n315 28 d2cmda2 not modelled 7.0 16 Fold:LDH C-terminal domain-like Superfamily:LDH C-terminal domain-like Family:Lactate & malate dehydrogenases, C-terminal domain 29 c1s1hJ_ not modelled 7.0 11 PDB header:ribosome Chain: J: PDB Molecule:40s ribosomal protein s20; PDBTitle: structure of the ribosomal 80s-eef2-sordarin complex from2 yeast obtained by docking atomic models for rna and protein3 components into a 11.7 a cryo-em map. this file, 1s1h,4 contains 40s subunit. the 60s ribosomal subunit is in file5 1s1i. 30 c2i3fA_ not modelled 6.7 20 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:glycolipid transfer-like protein; PDBTitle: crystal structure of a glycolipid transfer-like protein2 from galdieria sulphuraria 31 c2dc4A_ not modelled 6.7 23 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:165aa long hypothetical protein; PDBTitle: structure of ph1012 protein from pyrococcus horikoshii ot3 32 c3dcjA_ not modelled 6.5 14 PDB header:transferase Chain: A: PDB Molecule:probable 5'-phosphoribosylglycinamide PDBTitle: crystal structure of glycinamide formyltransferase (purn)2 from mycobacterium tuberculosis in complex with 5-methyl-5,3 6,7,8-tetrahydrofolic acid derivative 33 c2hjqA_ not modelled 6.5 14 PDB header:structural genomics Chain: A: PDB Molecule:hypothetical protein yqbf; PDBTitle: nmr structure of bacillus subtilis protein yqbf, northeast2 structural genomics target sr449 34 d1a6da3 not modelled 6.4 10 Fold:GroEL-intermediate domain like Superfamily:GroEL-intermediate domain like Family:Group II chaperonin (CCT, TRIC), intermediate domain 35 c1yrwA_ not modelled 5.4 14 PDB header:transferase Chain: A: PDB Molecule:protein arna; PDBTitle: crystal structure of e.coli arna transformylase domain 36 d1q3qa3 not modelled 5.4 14 Fold:GroEL-intermediate domain like Superfamily:GroEL-intermediate domain like Family:Group II chaperonin (CCT, TRIC), intermediate domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0