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Job DescriptionP04846
Confidence97.08%DateThu Jan 5 10:58:24 GMT 2012
Rank161Aligned Residues237
% Identity15%Templatec3osqA_
PDB info PDB header:fluorescent protein, transport proteinChain: A: PDB Molecule:maltose-binding periplasmic protein, green fluorescent PDBTitle: maltose-bound maltose sensor engineered by insertion of circularly2 permuted green fluorescent protein into e. coli maltose binding3 protein at position 175
Resolution1.90 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   30.........40.........50.........60......... 70.........80.. .......90.........100
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Query Sequence  DAKHIKVGVINGAEQDVAEVAKKVAKEKYGLDVELVGFSG. SLLPNDATNHGEL. DANVFQHRPFLEQDNQAH. . . . . . .
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Template Sequence  EEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPD
Template Known Secondary structure 
TTS

TTS





TTTT
S
SGGGTT
B





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   3......10.........20.........30.........40.........50.........60.........70.........80..
 
   101........110.........120........ .130.........140.........150.
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . GYKLVAVGNTFVFPMAGYSKKIKTVAQI. . . . . . . KEGATVAIPNDPTNLGRALLLLQ
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Template Sequence  KAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
Template Known Secondary structure  TTB
TTT
S

TTT
SS

SBSTTTTT




SS
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   83......90.........100.........110.........120.........130.........140.........150.........160..
 
   152.......160.........170.........180.
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Query Sequence  KEKLITLKEGKGLLPTALDITDNPRHLQIM. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  ADGGYAFKYENHLSHNVYIMADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNE
Template Known Secondary structure  TT

S


GGGTBTTS
SSSS





S

TT
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   163......170.........180.........190.........200.........210.........220.........230.........240..
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
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Template Sequence  KRDHMVLLEFVTAAGITLGMDELYKGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLP
Template Known Secondary structure 
SS




TT


TGGGGGSSTTTTTTTTSS
S
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   243......250.........260.........270.........280.........290.........300.........310.........320..
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation                                                                              
   
Template Sequence  VPWPTLVTTLVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNIL
Template Known Secondary structure  S
GGGGTTT

GGG
B

GGGGGG
TTTT
TTS
TTS

TTSTTT
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   329330.........340.........350.........360.........370.........380.........390.........400........
 
   182.......190.........200.........210........
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
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Template Sequence  GHKLEYNFNGGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYG
Template Known Secondary structure  TT
B
SSSTTTTSSSTTSS
TT

TTS
GGGT

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   412.......420.........430.........440.........450.........460.........470.........480.........490.
 
   219220...... ...230.........240.........250.........260......
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Query Sequence  VFIEDKNS. . . . . . . PYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNG
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Template Sequence  VTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
Template Known Secondary structure 


BBTTB



BTT
S
TTSSS
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   492.......500.........510.........520.........530.........540......
 
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No model constructed - rank, confidence too low




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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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