Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	K	T	I	Q	D	K	A	R	H	R	T	R	P	L	W	A	W	L	K	L	L	W	Q	R	I	D	E	D	N	M	T	T	L	A	G	N	L	A	Y	V	S	L	L	S	L	V	P	L	V	A	V	V	F	A	L	F	A	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	P	M	F	S	D	V	S	I	Q	L	R	H	F	I	F	A	N	F	L	P	A	T	G	D	V	I	Q	R	Y	I	E	Q	F	V	A	N	S	N	K	M	T	A	V	G	A	C	G	L	I	V	T	A	L	L	L	M	Y	S	I
Secondary structure
SS confidence
Disorder																			?			?	?														?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		D	S	A	L	N	T	I	W	R	S	K	R	A	R	P	K	I	Y	S	F	A	V	Y	W	M	I	L	T	L	G	P	L	L	A	G	A	S	L	A	I	S	S	Y	L	L	S	L	R	W	A	S	D	L	N	T	V	I	D	N	V
Secondary structure
SS confidence
Disorder																																																?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	R	I	F	P	L	L	L	S	W	I	S	F	W	L	L	Y	S	I	V	P	T	I	R	V	P	N	R	D	A	I	V	G	A	F	V	A	A	L	L	F	E	A	G	K	K	G	F	A	L	Y	I	T	M	F	P	S	Y	Q	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290
Sequence		I	Y	G	V	L	A	V	I	P	I	L	F	V	W	V	Y	W	T	W	C	I	V	L	L	G	A	E	I	T	V	T	L	G	E	Y	R	K	L	K	Q	A	A	E	Q	E	E	D	D	E	P
Secondary structure
SS confidence
Disorder																																						?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2r6g chain F domain 1 Region: 182 - 239 Aligned: 56 Modelled: 58 Confidence: 91.6% Identity: 20% Fold: MalF N-terminal region-like Superfamily: MalF N-terminal region-like Family: MalF N-terminal region-like Phyre2 PDB 2xq2 chain A Region: 29 - 290 Aligned: 222 Modelled: 241 Confidence: 23.2% Identity: 14% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt Phyre2 PDB 2qks chain A Region: 172 - 262 Aligned: 90 Modelled: 91 Confidence: 11.3% Identity: 17% PDB header:metal transport Chain: A: PDB Molecule:kir3.1-prokaryotic kir channel chimera; PDBTitle: crystal structure of a kir3.1-prokaryotic kir channel chimera Phyre2 PDB 1kf6 chain C Region: 4 - 158 Aligned: 126 Modelled: 136 Confidence: 7.0% Identity: 13% Fold: Heme-binding four-helical bundle Superfamily: Fumarate reductase respiratory complex transmembrane subunits Family: Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD) Phyre2 PDB 1a87 chain A Region: 17 - 92 Aligned: 74 Modelled: 76 Confidence: 6.6% Identity: 18% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 1a87 chain A Region: 17 - 92 Aligned: 74 Modelled: 76 Confidence: 6.6% Identity: 18% PDB header:bacteriocin Chain: A: PDB Molecule:colicin n; PDBTitle: colicin n Phyre2 PDB 1xl6 chain B Region: 172 - 262 Aligned: 90 Modelled: 91 Confidence: 6.3% Identity: 11% PDB header:metal transport Chain: B: PDB Molecule:inward rectifier potassium channel; PDBTitle: intermediate gating structure 2 of the inwardly rectifying k+ channel2 kirbac3.1 Phyre2 PDB 3b8e chain B Region: 238 - 257 Aligned: 20 Modelled: 20 Confidence: 6.2% Identity: 40% PDB header:hydrolase/transport protein Chain: B: PDB Molecule:sodium/potassium-transporting atpase subunit PDBTitle: crystal structure of the sodium-potassium pump Phyre2 PDB 3ixz chain B Region: 238 - 258 Aligned: 21 Modelled: 21 Confidence: 5.4% Identity: 29% PDB header:hydrolase Chain: B: PDB Molecule:potassium-transporting atpase subunit beta; PDBTitle: pig gastric h+/k+-atpase complexed with aluminium fluoride Phyre2 1 d2r6gf1 2 c2xq2A_ 3 c2qksA_ 4 d1kf6c_ 5 d1a87a_ 6 c1a87A_ 7 c1xl6B_ 8 c3b8eB_ 9 c3ixzB_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2r6gf1 91.6 20 Fold:MalF N-terminal region-like Superfamily:MalF N-terminal region-like Family:MalF N-terminal region-like 2 c2xq2A_ 23.2 14 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt 3 c2qksA_ 11.3 17 PDB header:metal transport Chain: A: PDB Molecule:kir3.1-prokaryotic kir channel chimera; PDBTitle: crystal structure of a kir3.1-prokaryotic kir channel chimera 4 d1kf6c_ 7.0 13 Fold:Heme-binding four-helical bundle Superfamily:Fumarate reductase respiratory complex transmembrane subunits Family:Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD) 5 d1a87a_ 6.6 18 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 6 c1a87A_ 6.6 18 PDB header:bacteriocin Chain: A: PDB Molecule:colicin n; PDBTitle: colicin n 7 c1xl6B_ 6.3 11 PDB header:metal transport Chain: B: PDB Molecule:inward rectifier potassium channel; PDBTitle: intermediate gating structure 2 of the inwardly rectifying k+ channel2 kirbac3.1 8 c3b8eB_ 6.2 40 PDB header:hydrolase/transport protein Chain: B: PDB Molecule:sodium/potassium-transporting atpase subunit PDBTitle: crystal structure of the sodium-potassium pump 9 c3ixzB_ 5.4 29 PDB header:hydrolase Chain: B: PDB Molecule:potassium-transporting atpase subunit beta; PDBTitle: pig gastric h+/k+-atpase complexed with aluminium fluoride

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0