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Job DescriptionP07762
Confidence100.00%DateThu Jan 5 11:00:31 GMT 2012
Rank5Aligned Residues629
% Identity19%Templatec2e8yA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:amyx protein; PDBTitle: crystal structure of pullulanase type i from bacillus subtilis str.2 168
Resolution2.11 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   37..40.........50.... .....60.........70. ........80......... 90.........100........
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Query Sequence  RALLPDATDVWVIEPKTG. . RKLAKLECLDSRGFFSG. VIPRRKNFFRYQLAVVWH. . . . . GQQNLIDDPYRFGPLIQEM
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Template Sequence  EAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYVCVSDHPVTFGKIHCVRASSGHKTDLQIG
Template Known Secondary structure  TTGGGTTTS
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TT
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TTS


TTS


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   910.........20.........30.........40.........50.........60.........70.........80........
 
   109110.........120.... .....130.........140.........150.........160.........170.........180.......
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Query Sequence  DAWLLSEGTHLRPYET. LGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRRHPMRLRKESGIWELFIPGAHNGQL
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Template Sequence  AVIRTAAFDDEFYYDGELGAVYTA. . . . DHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEK. GVYAVTVTGDLHGYE
Template Known Secondary structure  GGGGS


S


S....S
TT
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GG.GS

TT
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   8990.........100.........110.. .......120.........130.........140........ .150.........160...
 
   188.190.........200.........210.........220.........230.........240.........250.........260. ....
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Query Sequence  YKYEMIDANGNLRLKSDPYAFEAQMRPETASLICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNF. . WLSY
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Template Sequence  YLFCICN. NSEWMETVDQYAKAVTVNGEKGVVLRP. . DQMKWTAPLKPFSHPVD. AVIYETHLRDFSIHENSGMINKGKY
Template Known Secondary structure  .TT

TT
SSB
GGG
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S
GGG.


STTS

SSTTSG
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   164.....170 .........180.........190....... ..200.........210.... .....220.........230.........
 
   266...270. ........280.........290.... .....300.........310.... .....
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Query Sequence  RELADQ. . . . . . . . . . LVPYAKWMGFTHLELLPINEHPF. . . . . . . . DGSWGYQPTGLYAPTRRFGT. . . . . . . . RDDFR
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Template Sequence  LALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELK
Template Known Secondary structure  GGGG
TT
B
TTS
B
T
SS

SSS
TTSGGG





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STTSS
SSS
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   240.........250.........260.........270.........280.........290.........300.........310.........
 
   320.........330.........340...... ...350.........360.........370.........380.........390........
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Query Sequence  YFIDAAHAAGLNVILDWVPGHFPTDDF. ALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALYWIERFG
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Template Sequence  QMINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPGYFFRHDECGKPSNGTGVGNDIASERRMARKFIADCVVYWLEEYN
Template Known Secondary structure  TT

TT

SSGGGSSTTTSB
B
TTSSB

TTSSS

B
TTS
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   320.........330.........340.........350.........360.........370.........380.........390.........
 
   399400.........410.........420.........430.........440.........450.........460.........470........
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Query Sequence  IDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQVSGAVTMAEESTDFPGVSRPQDMGGLGFW
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Template Sequence  VDGFRFDLLGIL. . . . . . . . . . . . . . . . . . . . . . . . DIDTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAP
Template Known Secondary structure 

TTGGGS........................BSTT







SS
GGGB

GGGGG
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   400.........410. ........420.........430.........440.........450.....
 
   479480.........490 .........500.........510..... ....520.........530......
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Query Sequence  . . . . . YKWNLGWMHDTL. . . . . . . . . DYMKLDPVYRQYHHDKLTFGILYNY. . . . . . . . TENFVLPLSHDEVVHGKKSIL
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Template Sequence  RMPGIGFFNDMFRDAVKGNTFHLKATGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSF
Template Known Secondary structure  G
TT



SSSTT


GGGT
GGGBTTS
BTTB

SSSSGGG
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SSSS
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   456...460.........470.........480.........490.........500.........510.........520.........530.....
 
   537..540.........550.........560.........570.........580.. .......590.........600........
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Query Sequence  DRMPGDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNHDA. . . . . . . . SLDWHLLEGGDNWHHGVQRLVRDLNL
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Template Sequence  ALPQENDSRKRSRQRLAVAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWD. . . . RRETFKEDVHYIRRLIS
Template Known Secondary structure 
TT

TSBSTTGGGT


TT
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TT

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   536...540.........550.........560.........570.........580.........590.... .....600.........610.
 
   609610.........620.........630.........640........ .650.........660.........670.........680....
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Query Sequence  TYRHHKAMHELDFDPYGFEWLVVDDKERSVLIFVRRDKEG. . . . NEIIVASNFTPVPRHDYRFGINQPGKWREILNTDSM
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Template Sequence  LRKAHPAFRLRSAA. DIQRHLECLTLKEHLIAYRLYDLDEVDEWKDIIVIHHASPDSVE. . . WRLPNDIPYRLLCDP. . .
Template Known Secondary structure 
GGGG

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TTT
SSS
SSS...
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   612.......620..... ....630.........640.........650.........660......... 670.........680....
 
   685....690.........700.........710.........720....
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Query Sequence  HYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLV
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Template Sequence  . . . . . . . . . . SGFQEDPTEIKK. . . . . TVAVNGIGTVILY
Template Known Secondary structure  ..........T
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   685....690...... ...700.........
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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