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Job DescriptionP07762
Confidence100.00%DateThu Jan 5 11:00:31 GMT 2012
Rank21Aligned Residues538
% Identity15%Templatec2d0gA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha-amylase i; PDBTitle: crystal structure of thermoactinomyces vulgaris r-47 alpha-2 amylase 1 (tvai) mutant d356n/e396q complexed with p5, a3 pullulan model oligosaccharide
Resolution2.60 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   112.......120.........130.........140.........150.... .....160.........170.. .......180.....
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Query Sequence  LLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVG. QFNYWDGRRHPMRLRKES. . . . . GIWELFIPGAHNG
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Template Sequence  LFHDQGPLFDNAPEPTSTQS. . . . VTLKLRTFKGDITSANIKYWDTADNAFHWVPMVWDSNDPTGTFDYWKGTIPASPSI
Template Known Secondary structure 
B

SSTTTBS
SS

TT

....TT

STTTT

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SS
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   12.......20.........30. ........40.........50.........60.........70.........80.......
 
   186...190.........200.........210.........220.........230.........240.........250.........260.
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Query Sequence  QLYKYEMIDANGNLRLKSDPYAFEAQMRPETASLICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNF. . . .
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Template Sequence  KYYRFQINDG. TSTAWYNGNGPSSTEPNADDFYIIPNFKTPDWL. . . . . . . . . . . KNGVMYQIFPDRFYNGDSSNDVQTG
Template Known Secondary structure  T.TTTSS

SS


TT



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GGGT

S
GGG


TT
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   88.90....... ..100.........110.........120.........130 .........140.........150.....
 
   262.......270........ .280.........290.........300.........
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . WLSYRELADQLVPYAKW. MGFTHLELLPINEHPFDGSWGYQPTGLYAPT
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Template Sequence  SYTYNGTPTEKKAWGSSVYADPGYDNSLVFFGGDLAGIDQKL. GYIKKTLGANILYLNPIFKAPT. . NHKYDTQDYMAVD
Template Known Secondary structure  S
BSSS
B


TTS

S

TTS
GGG



T.TTT


S


SS..SS

S
S
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   156...160.........170.........180.........190....... ..200.........210......... 220.........230..
 
   310.........320......... 330.........340.........350..... ....360.........370.
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Query Sequence  RRFGTRDDFRYFIDAAHAAG. . . . LNVILDWVPGHFPTDDFALAEFDGTN. . . . . . . . . . . . . . LYEHSDPREGYHQDWN
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Template Sequence  PAFGDNSTLQTLINDIHSTANGPKGYLILDGVFNHTGDSHPWFDKYNNFSSQGAYESQSSPWYNYYTFYTWPDSYASFLG
Template Known Secondary structure  TTT


SSSSS



SB

TTSTTT
TT

SSS

TTT
TT
TTGGGB
TTT
TT
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   233......240.........250.........260.........270.........280.........290.........300.........310..
 
   372....... 380.........390. ........400.........410.........420.........430.........440.
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Query Sequence  . . . TLIYNYGR. . REVSNFLVGNAL. . . . . YWIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRN
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Template Sequence  FNSLPKLNYGNSGSAVRGVIYNNSNSVAKTYLNPPYSVDGWRLNAAQYV. . . . . . . . . . . . . DANGNNGSDVTNHQIWSE
Template Known Secondary structure  BB


STT
TTSTTSSTTT


TTGGG
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STT


SS
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   313......320.........330.........340.........350.........360. ........370.........
 
   442.......450.........460.........470.........480.........490.........500.. .......
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Query Sequence  TNRILGEQVSGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQY. . . . . . . . . . . . HHDKLTF
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Template Sequence  FRNAVKGVNSNAAIIGQYWGNANPWT. . . . AQGNQWDAATNFDGFTQPVSEWITGKDYQNNSASISTTQFDSWLRGTRAN
Template Known Secondary structure 
TT


SS

GGGT....TTSSS
SB
TTTTSB
TTS
B



TT
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   380.........390.........400..... ....410.........420.........430.........440.........450.....
 
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Query Sequence  GILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNH. . DASLDWH
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Template Sequence  YPTNVQQSMMNFLSNHDITRFATRSGGDL. . . . . . . . . WKTYLALIFQMTYVGTPTIYYGDEYGMQGGADPDNRRSFDWS
Template Known Secondary structure  S
TT
S

TTS

TTT
.........TTSSSTTTTTT



STTTTS



TT
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   456...460.........470.........480.... .....490.........500.........510.........520......
 
   588.590.........600.........610.........620.........630.........640.........650.........660......
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Query Sequence  LLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPYGFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYR.
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Template Sequence  . . . . QATPSNSAVALTQKLITIRNQYPALRTGSFM. . . . . . TLITDDTNKIYSYGRFDNV. NRIAVVLNNDSVSHTVNVP
Template Known Secondary structure  ....TSSTTS
S......TTTT
SS.

SSS

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   527..530.........540.........550....... ..560.........570...... ...580.........590.....
 
   667..670.........680.........690.........700.........710.........720.....
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Query Sequence  . . . FGINQPGKWREILNTDSMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVR
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Template Conservation               
 
 
    
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Template Sequence  VWQLSMPNGSTVTDKITGHSYTVQN. . . . . . . . . . . . . . . . . . . . GMVTVAVDGHYGAVLAQ
Template Known Secondary structure  GGGGT

TT
TTT


BT....................TB
TT
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   596...600.........610.........620 .........630.......
 
Download:Text version

No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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