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Job DescriptionP07762
Confidence100.00%DateThu Jan 5 11:00:31 GMT 2012
Rank24Aligned Residues427
% Identity19%Templatec1jgiA_
PDB info PDB header:transferaseChain: A: PDB Molecule:amylosucrase; PDBTitle: crystal structure of the active site mutant glu328gln of2 amylosucrase from neisseria polysaccharea in complex with3 the natural substrate sucrose
Resolution2.00 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   239240.........250.........260.........270.........280.........290....... ..300.........310.......
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Query Sequence  FDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYAKWMGFTHLELLPINEHPFDGS. WGYQPTGLYAPTRRFGTRDD
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Template Sequence  LSNKQVGGVCYVDLFA. . . . . . . . GDLKGLKDKI. PYFQELGLTYLYLMPLFKCPEGKSDGGYAVSSYRDVNPALGTIGD
Template Known Secondary structure  G
TT


........SST.T
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B


SS
STTTTS
S
SSSB
TTT

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   94.....100......... 110......... 120.........130.........140.........150.........160....
 
   318.320.........330.........340.........350 .........360..
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Query Sequence  FRYFIDAAHAAGLNVILDWVPGHFPTDDFALAE. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . FDGTNLYEHSDP
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Template Sequence  LREVIAALHEAGISAVVDFIFNHTSNEHEWAQRCAAGDPLFDNFYYIFPDRRMPDQYDRTLREIFPDQHPGGFSQLEDGR
Template Known Secondary structure  TT


STTST
GGGTTSB
SSSTS


S
TTT
STT
TTS
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   165....170.........180.........190.........200.........210.........220.........230.........240....
 
   363......370.........380.........390.........400.........410.........420.........430.........440..
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Query Sequence  REGYHQDWNTLIYNYGRREVSNFLVGNALYWIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNT
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Template Sequence  WVWTTFNSFQWDLNYSNPWVFRAMAGEMLFLA. NLGVDILRMDAVAFI. . . . . . . . . . . WKQMGTSCENLPQAHALIRAF
Template Known Secondary structure 
SS
TTB
TTS.TTT

TGGGS...........


TTS
SSS
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   245....250.........260.........270...... ...280.........290. ........300.........310..
 
   443......450.........460.........470.........480.........490.........500.........510.........520..
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Query Sequence  NRILGEQVSGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLTFGILYNYTENFVLP
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Template Sequence  NAVMRIAAPAVFFKSQAIVHPDQVVQYI. . GQDECQIGYNPLQMALLWNTLATR. EVNLLHQALTYRHNLPEHTAWVNYV
Template Known Secondary structure 
TT


S
GGGB..STTSBS

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TT
S
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   313......320.........330.........340 .........350.........360.... .....370.........380.........
 
   523......530 .........540.........550.
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Query Sequence  LSHDEVVH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GKKSILDRMPGDAWQKFANLR
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Template Conservation    

                                                       
                 
  
Template Sequence  RSHDDIGWTFADEDAAYLGISGYDHRQFLNRFFVNRFDGSFARGVPFQYNPSTGDCRVSGTAAALVGLAQDDPHAVDRIK
Template Known Secondary structure 

SS
B




TT

TT
STT







TTT




TTTTT
TT
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   390.........400.........410.........420.........430.........440.........450.........460.........
 
   552.......560.........570.........580 .........590 .........600.........610....
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Query Sequence  AYYGWMWAFPGKKLLFMGNEFAQGREWNH. . . . . . . . . . . . . . DASLDWHLLE. . . GGDNWHHGVQRLVRDLNLTYRHHK
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Template Sequence  LLYSIALSTGGLPLIYLGDEVGTLNDDDWSQDSNKSDDSRWAHRPRYNEALYAQRNDPSTAAGQIYQDLRHMIAVRQSNP
Template Known Secondary structure  SSSTTGGGT



SSGGG
TTTTT
GGGGG




TTTT
TTS
G
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   470.........480.........490.........500.........510.........520.........530.........540.........
 
   615....620.........630.........640.........650.........660.........670.. .......680.........690..
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Query Sequence  AMHELDFDPYGFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQP. . GKWREILNTDSMHYHGSNAG
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Template Sequence  RFDGGRLV. . . . . . . TFNTNNKHIIGYIR. . . . NNALLAFGNFSEYPQT. VTAHTLQAMPFKAHDLIGGKTVSLNQ. . . .
Template Known Secondary structure  GG
SS
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S
TT....TTT
SSS
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TTTTTTS
STTT

SSS....
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   550....... ..560.........570. ........580....... ..590.........600.........610...
 
   693......700.........710.........720....
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Query Sequence  NGGTVHSDEIASHGRQHSLSLTLPPLATIWLV
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Template Sequence  . . . . . . . . . . . . . . . . . . . DLTLQPYQVMWLE
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TT
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   614.....620......
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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