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Job DescriptionP07762
Confidence100.00%DateThu Jan 5 11:00:31 GMT 2012
Rank52Aligned Residues416
% Identity17%Templatec1jd7A_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha-amylase; PDBTitle: crystal structure analysis of the mutant k300r of2 pseudoalteromonas haloplanctis alpha-amylase
Resolution2.25 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   241........250.........260.........270.........280.........290........ .300.........310........
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Query Sequence  APISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYAKWMGFTHLELLPINEHPFDGSW. . GYQPTGLYAPTRRFGTRDDF
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Template Sequence  TPTTF. . VHLFEWN. . . . . . . . . . WQDVAQECEQYLGPKGYAAVQVSPPNEHITGSQWWTRYQPVSY. ELQSRGGNRAQF
Template Known Secondary structure 



..TT

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S
BB
SSSGGGGGSBS
S.SS
BTTB
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   1.... ....10.. .......20.........30.........40.........50..... ....60.......
 
   319320.........330.........340.........350.........360.........370. ......
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Query Sequence  RYFIDAAHAAGLNVILDWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWN. . . . . . . . . . . . . . . . . . . . . TLIYNY
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Template Sequence  IDMVNRCSAAGVDIYVDTLINHMAAGSGTGTAGNSFGNKSFPIYSPQDFHESCTINNSDYGNDRYRVQNCELVGLADLDT
Template Known Secondary structure  TT

S
SS
BTTS
BTTB
SS

GGGB



B


SBBTTBB
T
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   68.70.........80.........90.........100.........110.........120.........130.........140.......
 
   378.380.........390.........400.........410.........420.........430.........440.........450.......
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Query Sequence  GRREVSNFLVGNALYWIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQVSGAVTMA
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Template Sequence  ASNYVQNTIAAYINDLQ. AIGVKGFRFDASKHVAA. . . . . . . . . . . . . . . . . . . . . . . . SDIQSLMAKV. . . NGSPVVFQ
Template Known Secondary structure  TS.T

TGGGS
........................TTS...
S

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   148.150.........160.... .....170.........180. ........190. ........
 
   458.460.........470.........480.........490.........500.........510.........520.........530.......
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Query Sequence  EESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLTFGILYNYTENFVLPLSHDEVVHGKKSILD
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Template Sequence  EVIDQGGEAVGASEYLST. . GLVTEFKYSTELGNTFRNGSLAWLSN. . FGEGWGFMPSSSAVVFVDNHDNQRGHGGAGNV
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SS
SS
GGGGTTT..S
S
GGGGGG..
SGGGT


GGG
S

TTGGGTSS

TTS
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   200.........210....... ..220.........230.........240... ......250.........260.........270.....
 
   538.540.........550.........560. ........570.........580.... .....590.........600.........610..
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Query Sequence  RMPGDAWQKFANLRAYYGWMWAFP. GKKLLFMGNEFAQGREWNHDASL. . . . DWHLLEGGDNWHHGVQRLVRDLNLTYRH
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Template Sequence  ITFEDG. . . . RLYDLANVFMLAYPYGYPRVMSSYDFHGDTDAGGPNVPVHNNGNLECFASNWKCEHRWSYIAGGVDFRNN
Template Known Secondary structure 

GGGT....S

S
B

TT
TT



SS
STT
BSSSSB

GGGST
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   276...280. ........290.........300.........310.........320.........330.........340.........350.
 
   613......620.........630.........640.........650.........660... ......670.........680.........690.
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Query Sequence  HKAMHELDFDPYGFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRH. DYRFGINQPGKWREILNTDSMHYHGSNA
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Template Sequence  TA. . . . . . . . . DNWAVTNWWDNTNNQISFGR. . . . GSSGHMAINKEDSTLTATVQTDMA. SGQYCNVLKGELSADAKSCS
Template Known Secondary structure  TT.........T




SSS
....GGG
SSS
B



S
.S
TTT

B
TTSS
BS
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   352. ......360.........370... ......380.........390....... ..400.........410.......
 
   692.......700.........710.........720.......
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Query Sequence  GNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREA
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Template Sequence  GEVITVNS. . . . . . . . DGTINLNIGAWDAMAIHKNA
Template Known Secondary structure  S

T........TS

B
TTTTS
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   418.420..... ....430.........440.....
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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