Secondary structure and disorder prediction

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Sequence		M	D	F	D	T	I	M	E	K	A	Y	E	E	Y	F	E	G	L	A	E	G	E	E	A	L	S	F	S	E	F	K	Q	A	L	S	S	S	A	K	S	N	G
Secondary structure
SS confidence
Disorder		?	?																																	?	?	?		?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3fka chain D Region: 2 - 25 Aligned: 23 Modelled: 24 Confidence: 24.9% Identity: 9% PDB header:unknown function Chain: D: PDB Molecule:uncharacterized ntf-2 like protein; PDBTitle: crystal structure of a ntf-2 like protein of unknown function2 (spo1084) from silicibacter pomeroyi dss-3 at 1.69 a resolution Phyre2 PDB 3duk chain D Region: 2 - 25 Aligned: 23 Modelled: 24 Confidence: 24.0% Identity: 26% PDB header:unknown function Chain: D: PDB Molecule:ntf2-like protein of unknown function; PDBTitle: crystal structure of a ntf2-like protein of unknown function2 (mfla_0564) from methylobacillus flagellatus kt at 2.200 a resolution Phyre2 PDB 1k94 chain A Region: 13 - 36 Aligned: 24 Modelled: 24 Confidence: 21.8% Identity: 21% Fold: EF Hand-like Superfamily: EF-hand Family: Penta-EF-hand proteins Phyre2 PDB 2qti chain A domain 1 Region: 28 - 41 Aligned: 14 Modelled: 14 Confidence: 18.3% Identity: 50% Fold: YejL-like Superfamily: YejL-like Family: YejL-like Phyre2 PDB 2juw chain A domain 1 Region: 28 - 41 Aligned: 14 Modelled: 14 Confidence: 15.8% Identity: 50% Fold: YejL-like Superfamily: YejL-like Family: YejL-like Phyre2 PDB 2jxc chain A domain 1 Region: 8 - 37 Aligned: 30 Modelled: 30 Confidence: 15.6% Identity: 27% Fold: EF Hand-like Superfamily: EF-hand Family: Eps15 homology domain (EH domain) Phyre2 PDB 2kq6 chain A Region: 16 - 40 Aligned: 25 Modelled: 25 Confidence: 13.5% Identity: 32% PDB header:transport protein Chain: A: PDB Molecule:polycystin-2; PDBTitle: the structure of the ef-hand domain of polycystin-2 suggests a2 mechanism for ca2+-dependent regulation of polycystin-2 channel3 activity Phyre2 PDB 2kfh chain A Region: 8 - 37 Aligned: 30 Modelled: 30 Confidence: 11.3% Identity: 20% PDB header:protein binding Chain: A: PDB Molecule:eh domain-containing protein 1; PDBTitle: structure of the c-terminal domain of ehd1 with fnyestgpftak Phyre2 PDB 1dvi chain A Region: 8 - 36 Aligned: 29 Modelled: 29 Confidence: 11.3% Identity: 24% Fold: EF Hand-like Superfamily: EF-hand Family: Penta-EF-hand proteins Phyre2 PDB 1alv chain A Region: 8 - 36 Aligned: 29 Modelled: 29 Confidence: 10.0% Identity: 21% Fold: EF Hand-like Superfamily: EF-hand Family: Penta-EF-hand proteins Phyre2 PDB 1mqs chain B Region: 28 - 41 Aligned: 14 Modelled: 14 Confidence: 8.8% Identity: 50% PDB header:endocytosis/exocytosis Chain: B: PDB Molecule:integral membrane protein sed5; PDBTitle: crystal structure of sly1p in complex with an n-terminal2 peptide of sed5p Phyre2 PDB 1g33 chain A Region: 20 - 37 Aligned: 18 Modelled: 18 Confidence: 8.3% Identity: 22% Fold: EF Hand-like Superfamily: EF-hand Family: Parvalbumin Phyre2 PDB 1gjy chain A Region: 13 - 36 Aligned: 24 Modelled: 24 Confidence: 8.2% Identity: 21% Fold: EF Hand-like Superfamily: EF-hand Family: Penta-EF-hand proteins Phyre2 PDB 3fhk chain F Region: 7 - 20 Aligned: 14 Modelled: 14 Confidence: 8.0% Identity: 50% PDB header:structural genomics, unknown function Chain: F: PDB Molecule:upf0403 protein yphp; PDBTitle: crystal structure of apc1446, b.subtilis yphp disulfide2 isomerase Phyre2 PDB 1qjt chain A Region: 11 - 37 Aligned: 27 Modelled: 27 Confidence: 7.4% Identity: 22% Fold: EF Hand-like Superfamily: EF-hand Family: Eps15 homology domain (EH domain) Phyre2 PDB 2kle chain A Region: 16 - 36 Aligned: 21 Modelled: 21 Confidence: 7.2% Identity: 38% PDB header:membrane protein Chain: A: PDB Molecule:polycystin-2; PDBTitle: isic refined solution structure of the calcium binding2 domain of the c-terminal cytosolic domain of polycystin-2 Phyre2 PDB 1kxp chain D domain 3 Region: 25 - 41 Aligned: 17 Modelled: 17 Confidence: 5.9% Identity: 29% Fold: Serum albumin-like Superfamily: Serum albumin-like Family: Serum albumin-like Phyre2 PDB 1lkj chain A Region: 8 - 35 Aligned: 28 Modelled: 28 Confidence: 5.6% Identity: 14% Fold: EF Hand-like Superfamily: EF-hand Family: Calmodulin-like Phyre2 PDB 2pmy chain B Region: 8 - 37 Aligned: 30 Modelled: 30 Confidence: 5.5% Identity: 30% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:ras and ef-hand domain-containing protein; PDBTitle: ef-hand domain of human rasef Phyre2 PDB 2vrg chain A Region: 19 - 34 Aligned: 16 Modelled: 16 Confidence: 5.4% Identity: 19% PDB header:transport Chain: A: PDB Molecule:multiple coagulation factor deficiency protein 2; PDBTitle: structure of human mcfd2 Phyre2 1 c3fkaD_ 2 c3dukD_ 3 d1k94a_ 4 d2qtia1 5 d2juwa1 6 d2jxca1 7 c2kq6A_ 8 c2kfhA_ 9 d1dvia_ 10 d1alva_ 11 c1mqsB_ 12 d1g33a_ 13 d1gjya_ 14 c3fhkF_ 15 d1qjta_ 16 c2kleA_ 17 d1kxpd3 18 d1lkja_ 19 c2pmyB_ 20 c2vrgA_ 21 22 23 24 25

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3fkaD_ 24.9 9 PDB header:unknown function Chain: D: PDB Molecule:uncharacterized ntf-2 like protein; PDBTitle: crystal structure of a ntf-2 like protein of unknown function2 (spo1084) from silicibacter pomeroyi dss-3 at 1.69 a resolution 2 c3dukD_ 24.0 26 PDB header:unknown function Chain: D: PDB Molecule:ntf2-like protein of unknown function; PDBTitle: crystal structure of a ntf2-like protein of unknown function2 (mfla_0564) from methylobacillus flagellatus kt at 2.200 a resolution 3 d1k94a_ 21.8 21 Fold:EF Hand-like Superfamily:EF-hand Family:Penta-EF-hand proteins 4 d2qtia1 18.3 50 Fold:YejL-like Superfamily:YejL-like Family:YejL-like 5 d2juwa1 15.8 50 Fold:YejL-like Superfamily:YejL-like Family:YejL-like 6 d2jxca1 15.6 27 Fold:EF Hand-like Superfamily:EF-hand Family:Eps15 homology domain (EH domain) 7 c2kq6A_ 13.5 32 PDB header:transport protein Chain: A: PDB Molecule:polycystin-2; PDBTitle: the structure of the ef-hand domain of polycystin-2 suggests a2 mechanism for ca2+-dependent regulation of polycystin-2 channel3 activity 8 c2kfhA_ 11.3 20 PDB header:protein binding Chain: A: PDB Molecule:eh domain-containing protein 1; PDBTitle: structure of the c-terminal domain of ehd1 with fnyestgpftak 9 d1dvia_ 11.3 24 Fold:EF Hand-like Superfamily:EF-hand Family:Penta-EF-hand proteins 10 d1alva_ 10.0 21 Fold:EF Hand-like Superfamily:EF-hand Family:Penta-EF-hand proteins 11 c1mqsB_ 8.8 50 PDB header:endocytosis/exocytosis Chain: B: PDB Molecule:integral membrane protein sed5; PDBTitle: crystal structure of sly1p in complex with an n-terminal2 peptide of sed5p 12 d1g33a_ 8.3 22 Fold:EF Hand-like Superfamily:EF-hand Family:Parvalbumin 13 d1gjya_ 8.2 21 Fold:EF Hand-like Superfamily:EF-hand Family:Penta-EF-hand proteins 14 c3fhkF_ 8.0 50 PDB header:structural genomics, unknown function Chain: F: PDB Molecule:upf0403 protein yphp; PDBTitle: crystal structure of apc1446, b.subtilis yphp disulfide2 isomerase 15 d1qjta_ 7.4 22 Fold:EF Hand-like Superfamily:EF-hand Family:Eps15 homology domain (EH domain) 16 c2kleA_ 7.2 38 PDB header:membrane protein Chain: A: PDB Molecule:polycystin-2; PDBTitle: isic refined solution structure of the calcium binding2 domain of the c-terminal cytosolic domain of polycystin-2 17 d1kxpd3 5.9 29 Fold:Serum albumin-like Superfamily:Serum albumin-like Family:Serum albumin-like 18 d1lkja_ 5.6 14 Fold:EF Hand-like Superfamily:EF-hand Family:Calmodulin-like 19 c2pmyB_ 5.5 30 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:ras and ef-hand domain-containing protein; PDBTitle: ef-hand domain of human rasef 20 c2vrgA_ 5.4 19 PDB header:transport Chain: A: PDB Molecule:multiple coagulation factor deficiency protein 2; PDBTitle: structure of human mcfd2 21 d1a03a_ not modelled 5.2 15 Fold:EF Hand-like Superfamily:EF-hand Family:S100 proteins 22 d1c7va_ not modelled 5.2 22 Fold:EF Hand-like Superfamily:EF-hand Family:Calmodulin-like 23 d5pala_ not modelled 5.2 11 Fold:EF Hand-like Superfamily:EF-hand Family:Parvalbumin 24 d1snla_ not modelled 5.1 12 Fold:EF Hand-like Superfamily:EF-hand Family:EF-hand modules in multidomain proteins 25 d1a4pa_ not modelled 5.1 22 Fold:EF Hand-like Superfamily:EF-hand Family:S100 proteins

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0