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Job DescriptionP28904
Confidence100.00%DateThu Jan 5 11:45:17 GMT 2012
Rank36Aligned Residues470
% Identity20%Templatec2fhfA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:pullulanase; PDBTitle: crystal structure analysis of klebsiella pneumoniae2 pullulanase complexed with maltotetraose
Resolution1.65 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   8.10.........20......... 30........ .40.........50.........60
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Query Sequence  WQNGVIYQIYPKSFQDTTGSGT. . . . GDLRGVIQH. . . . . . . LDYLHKLGVDAIWLTPFYVSPQ. . . . . . . . . . . . . . . .
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Template Sequence  LAKMTIHESHIRDLSAWDQTVPAELRGKYLALTAQESNMVQHLKQLSASGVTHIELLPVFDLATVNEFSDKVADIQQPFS
Template Known Secondary structure  T

TT
TTS
GGGTTSGGGGG
TTST

S

SSS


GGG


TTSB
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   421........430.........440.........450.........460.........470.........480.........490.........500
 
   61........70.........
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . VDNGYDVANYTAIDPTYGT. . . . . . .
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Template Sequence  RLCEVNSAVKSSEFAGYCDSGSTVEEVLTQLKQNDSKDNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDPEGTAR
Template Known Secondary structure 
TSTTGGGTTSS
BSTT
BTTB
TTSSS






STTSS

STT
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   501........510.........520.........530.........540.........550.........560.........570.........580
 
   80.........90.. .......100.........110.........120.........130.........140.........150........
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Query Sequence  LDDFDELVTQAKS. RGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQ
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Template Sequence  IKEFRTMIQAIKQDLGMNVIMDVVYNHTNAAGPTDRTSVLDKIV. PWYYQRLNETT. . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  TS


TTS

SSS
TT

S.TTTSB
B
TTT........................
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   581........590.........600.........610.........620.... .....630.....
 
   159160.........170.........180.........190 .........200.........210.........220.........230.......
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Query Sequence  YYLHLFAPEQADLNWENPAVRAELKKVCEFWA. DRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMN
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Template Sequence  . GSVESATCCSDSAPEHRMFAKLIADSLAVWTTDYKIDGFRFDLMLYHPK. . . . . . . . . . . . . . . . . . . . . . . AQILSAW
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B

TTSSB
TTS


TTGGGSB.......................
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   636...640.........650.........660.........670.........680.... .....690.
 
   238.240.. .......250...... ...260.........270.........280.........290.
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Query Sequence  RDVFT. PRGLMTVGEMSSTS. . . . . . . . . . . . . . . . . . . . . . . . . LEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWT
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Template Sequence  ERIKALNPDIYFFGEGWDSNQSDRFEIASQINLKGTGIGTFSDRLRDAVRGGGPFDSGDALRQNQGVGSGAGVLPNELTT
Template Known Secondary structure  TT
TT





S
TTTS
B

TTTTTTSS


S
TT

TT


GGGTTTTS

SS

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   692.......700.........710.........720.........730.........740.........750.........760.........770.
 
   292.......300.........310 .........320.........330... ...
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Query Sequence  LAKPDFVALKTLFRHWQQG. . . . . . . . . . . . . . . . . . . . . . . . . . . . . MHNVAWNALFWCNHDQPRIVSRF. . . . . . GDE
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Template Sequence  LSDDQARHLADLTRLGMAGNLADFVLIDKDGAVKRGSEIDYNGAPGGYAADPTEVVNYVSKHDNQTLWDMISYKAAQEAD
Template Known Secondary structure 

TT
BTT

TTS
GGGSTTS

BSSGGG
S

SSSS
S
TT

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   772.......780.........790.........800.........810.........820.........830.........840.........850.
 
   337..340.........350.........360.........370.........380.........390.........400.........410......
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Query Sequence  GEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADELLAILASKSRDNSRTP
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Template Sequence  LDTRVRMQAVSLATVMLGQGIAFDQQGSELLRSKSFTRDSYDSGDWFNRVDYS. . . . . . . . . . . . . . . . . . . . . . . . LQD
Template Known Secondary structure  TSSSTTGGGT


TT
S

TTS


TT........................
S
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   852.......860.........870.........880.........890.........900.... ...
 
   417..420.........430.........440.........450.........460.........470.........480. .....
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Query Sequence  MQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALADDSSVFYTYQKLIALRKQEAILTWGNYQDLL. . . . . . . . . . PNSPV
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Template Sequence  NNYNVGMPRSSDDGSNYDIIAR. VKDAVATPGETELKQMTAFYQELTALRKSSPLFTLGDGATVMKRVDFRNTGADQQTG
Template Known Secondary structure 
S
SSS


GGG.TTSS



TS
GGGG


S
STT

TT
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   908.910.........920......... 930.........940.........950.........960.........970.........980......
 
   487..490...... ...500.........510.........520... ......530.........540........
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Query Sequence  LWCYRREWKG. . . . . . . . . . QTLLVIANLSREIQPWQAGQMRGNWQL. . . . . . . . VMHNYEEASPQPCAMNLRPFEAVWW
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Template Sequence  LLVMTIDDGMQAGASLDSRVDGIVVAINAAPESRTLQDFAGTSLQLSAIQQAAGDRSLASGVQVAADGSVTLPAWSVAVL
Template Known Secondary structure 
STTTSS
S
TT
SSS

TTSSS


TGGGSTTTT

TTS

TT
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   987..990.........1000.........1010.........1020.........1030.........1040.........1050.........1060......
 
   549550.
Predicted Secondary structure 
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Query Sequence  LQK
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Template Sequence  ELP
Template Known Secondary structure 
Template Predicted Secondary structure 
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   1067..
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
© Structural Bioinformatics Group, Imperial College, London
Lawrence Kelley, Benjamin Jefferys 
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