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Job DescriptionP28904
Confidence100.00%DateThu Jan 5 11:45:17 GMT 2012
Rank30Aligned Residues432
% Identity19%Templatec2e8yA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:amyx protein; PDBTitle: crystal structure of pullulanase type i from bacillus subtilis str.2 168
Resolution2.11 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   5....10.........20......... 30........ .40.........50......... 60.
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Query Sequence  PHWWQNGVIYQIYPKSFQDTTGSGT. . . GDLRGVIQH. . . . . . . . . . . LDYLHKLGVDAIWLTPFYVSP. . . . . . . . . QV
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Template Sequence  FSHPVDAVIYETHLRDFSIHENSGMINKGKYLALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAY
Template Known Secondary structure 
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GGG


STTS

SSTTSGGGGG
TT
B
TTS
B
T
SS

SSS
TTSGGG

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   209210.........220.........230.........240.........250.........260.........270.........280........
 
   62.......70......... 80.........90.........100.........110.........120.........130...
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Query Sequence  DNGYDVANYTAIDPTYGT. . . . . . . . LDDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGE
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Template Sequence  NWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENSPFEKT. . . . . VPGYFFRHDEC
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S
STTSS
SSSTT

TT

SSGGGS.....STTTSB
B
TT
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   289290.........300.........310.........320.........330.........340.........350.. .......360...
 
   134.....140.........150.........160.........170.........180.........190 .........200.........210..
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Query Sequence  PETPPNNWRSKFGGSAWRWHAESEQYYLHLFAPEQADLNWENPAVRAELKKVCEFWA. DRGVDGLRLDVVNLISKDPRFP
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Template Sequence  G. . . . . . . . . . . . . . . . . . . . . . . . . KPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFRFDLLGILDI. . . . .
Template Known Secondary structure  S.........................SB

TTSSS

B
TTS


TTGGGSB.....
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   364 .....370.........380.........390.........400.........410...
 
   213......220.........230.........240.. .......250.........260.........270.........280........
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Query Sequence  EDLDGDGRRFYTDGPRAHEFLHEMNRDVFT. PRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGE. . .
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Template Sequence  . . . . . . . . . . . . . . . . . . DTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNT
Template Known Secondary structure  ..................STT







SS
GGGB

GGGGGG
TT



S
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   414.....420.........430.........440.........450.........460.........470.....
 
   289290.........300.........310 .........320.........330... ......340.........350
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Query Sequence  . . . . . . . KWTLAKPDFVALKTLFRHWQQG. . . . . . MHNVAWNALFWCNHDQPRIVSRF. . . . . GDEGEYRVPAAKMLAMV
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Template Sequence  FHLKATGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQRLAVAI
Template Known Secondary structure  SSTT


GGGT
GGGBTTS
BTTB

SSSSGGG
S

SSSS

TT

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   476...480.........490.........500.........510.........520.........530.........540.........550.....
 
   351........360.........370.........380.........390.........400.........410.........420.........430
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Query Sequence  LHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAG
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Template Sequence  ILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWD. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  TSBSTTGGGT


TT
S

TT

S

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   556...560.........570.........580.........590....
 
   431........440.........450.........460.........470....... ..480.........490...... ...
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Query Sequence  EPWIGLGDNYQQINVEAALADDSSVFYTYQKLIALRKQEAILTWGNY. . . . . . QDLLPNSPVLWCYRREWKG. . . . . QTL
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Template Sequence  . . . . . . . . . . . . . . . . . RRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHLIAYRLYDLDEVDEWKDI
Template Known Secondary structure  .................T
GGGG

S
SS


TTT
SSS
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   595....600.........610.........620.........630.........640.........650.......
 
   500.........510.........520....... ..530.........540........
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Query Sequence  LVIANLSREIQPWQAGQMRGNWQLVMHN. . . YEEASPQPCAMNLRPFEAVWW
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Template Sequence  IVIHHASPDSVEWRLPN. DIPYRLLCDPSGFQEDPTEIKKTVAVNGIGTVIL
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SSS
SS.
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   658.660.........670.... .....680.........690.........700........
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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