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Job DescriptionP28904
Confidence100.00%DateThu Jan 5 11:45:17 GMT 2012
Rank19Aligned Residues444
% Identity25%Templatec2d0gA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha-amylase i; PDBTitle: crystal structure of thermoactinomyces vulgaris r-47 alpha-2 amylase 1 (tvai) mutant d356n/e396q complexed with p5, a3 pullulan model oligosaccharide
Resolution2.60 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   3......10.........20......... 30.........40.... .
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Query Sequence  HLPHWWQNGVIYQIYPKSFQDTTGSGT. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GDLRGVIQHLDYLHK. L
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Template Sequence  KTPDWLKNGVMYQIFPDRFYNGDSSNDVQTGSYTYNGTPTEKKAWGSSVYADPGYDNSLVFFGGDLAGIDQKLGYIKKTL
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GGGT

S
GGG


TTS
BSSS
B


TTS

S

TTS
GGG



TTTT
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   125....130.........140.........150.........160.........170.........180.........190.........200....
 
   46...50.........60.........70.........80.........90.... .....100.........110........
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Query Sequence  GVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRG. . . . IRIILDMVFNHTSTQHAWFREALN. . .
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Template Sequence  GANILYLNPIFKA. PTNHKYDTQDYMAVDPAFGDNSTLQTLINDIHSTANGPKGYLILDGVFNHTGDSHPWFDKYNNFSS
Template Known Secondary structure 


S


.SSSS

S
S
TTT


SSSSS



SB

TTSTTT
TT

SSS
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   205....210....... ..220.........230.........240.........250.........260.........270.........280...
 
   119120.........130.........140.........150.........160.........170..... ....180.......
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Query Sequence  . . . . . . KESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYYLHLFAPEQADLNWEN. . PAVRAELKKVCE. . .
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Template Sequence  QGAYESQSSPWYNYYTFYTWPD. . . . . . . . . . . . . . . . . . . . . . . SYASFLGFNSLPKLNYGNSGSAVRGVIYNNSNSVA
Template Known Secondary structure 

TTT
TT
TTGGGB
TTT.......................
TTBB


STT
TTSTTS
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   284.....290.........300..... ....310.........320.........330.........340
 
   188.190 .........200.........210.........220.........230.........240.. .......250.........260...
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Query Sequence  . . FWA. DRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDVFT. PRGLMTVGEMSSTSLEHCQRY
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Template Sequence  KTYLNPPYSVDGWRLNAAQYVDAN. . . . . . . . . . . . GNNGSDVTNHQIWSEFRNAVKGVNSNAAIIGQYWGNANPWTAQG
Template Known Secondary structure  STTT


TTGGG

ST............T


SS
TT


SS

GGGTTTS
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   341........350.........360.... .....370.........380.........390.........400........
 
   264.....270.........280.........290.........300.........310....... ..320.........330.........340.
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Query Sequence  AALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWN. . ALFWCNHDQPRIVSRFGDEGEYRV
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Template Sequence  NQWDAATNFDGFTQPVSEWITGKDYQNNSASISTTQFDSWLRGTRANYPTNVQQSMMNFLSNHDITRFATRSGG. . . . DL
Template Known Secondary structure  SS
SB
TTTTSB
TTS
B



TTS
TT
S

TTS

TTT....
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   409410.........420.........430.........440.........450.........460.........470.........480.. ..
 
   342.......350.........360.........370.........380.........390.........400.........410.........420.
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Query Sequence  PAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADELLAILASKSRDNSRTPMQWSN
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Template Sequence  WKTYLALIFQMTYVGTPTIYYGDEYGMQGGADPD. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . NRRSFDWS.
Template Known Secondary structure  TTSSSTTTTTT



STTT.....................................TS



TT.
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   485....490.........500.........510........ .520......
 
   422.......430.........440.........450.........460.........470.........480.... .....490.........500
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Query Sequence  GDNAGFTAGEPWIGLGDNYQQINVEAALADDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNS. PVLWCYRREWKGQTLL
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . QATPSNSAVALTQKLITIRNQYPALRTGSFMTLITDDTNKIYSYGRFDNVNRIA
Template Known Secondary structure  ..........................TSSTTS
STTTT
SS
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   527..530.........540.........550.........560.........570.........580
 
   501........510........ .520......... 530.........540.........550
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Query Sequence  VIANLSREIQPWQAGQMR. . . . . . GNWQLVMHNYE. EASPQPCAMNLRPFEAVWWLQ
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Template Sequence  VVLNNDSVSHTVNVPVWQLSMPNGSTVTDKITGHSYTVQNGMVTVAVDGHYGAVLAQ
Template Known Secondary structure 
SSS

GGGGT

TT
TTT


BTTB
TT
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   581........590.........600.........610.........620.........630.......
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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