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Job DescriptionP28904
Confidence100.00%DateThu Jan 5 11:45:17 GMT 2012
Rank40Aligned Residues431
% Identity21%Templatec2aaaA_
PDB info PDB header:glycosidaseChain: A: PDB Molecule:alpha-amylase; PDBTitle: calcium binding in alpha-amylases: an x-ray diffraction2 study at 2.1 angstroms resolution of two enzymes from3 aspergillus
Resolution2.10 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   5....10.........20......... 30.........40.........50........ .60.....
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Query Sequence  PHWWQNGVIYQIYPKSFQDTTGSGT. . . . . . . . . . . . GDLRGVIQHLDYLHKLGVDAIWLTPFYVS. . . . . . . PQVDNGY
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Template Sequence  ASW. RTQSIYFLLTDRFGRTDNSTTATCNTGNEIYCGGSWQGIIDHLDYIEGMGFTAIWISPITEQLPQDTADGEAYHGY
Template Known Secondary structure  .TT





TT

S




GGG
S




TTTT








BTTB
STTS
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   4.. ...10.........20.........30.........40.........50.........60.........70.........80..
 
   66...70.........80.........90.........100.........110.........120.........130.........140.....
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Query Sequence  DVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKF
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Template Sequence  WQQKIYDVNSNFGTADNLKSLSDALHARGMYLMVDVVPDHMGYAGNGNDVDYSVFDPFDSSSYFHPYCL. . . . . . . . . . .
Template Known Secondary structure  S
TTT

TTT


SB

BSS
GGG

GGGSBS

SGGGB



B...........
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   83......90.........100.........110.........120.........130.........140.........150.
 
   146...150.........160.........170.........180.........190 .........200.........210.........220....
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Query Sequence  GGSAWRWHAESEQYYLHLFAPEQADLNWENPAVRAELKKVCEFWA. DRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYT
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Template Sequence  . ITDWDNLTMVEDCWEGDTIVSLPDLDTTETAVRTIWYDWVADLVSNYSVDGLRIDSVLEVQPD. . . . . . . . . . . . . . . .
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TT
SB
SSS
BB
TTST

S
STTS
GG................
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   152.......160.........170.........180.........190.........200.........210....
 
   225....230.........240.........250.........260.........270.........280.........290.........300....
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Query Sequence  DGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVALKTLF
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Template Sequence  . . . . . . . FFPGYNKAS. . . . GVYCVGEIDNGNPASDCPYQKVL. . . . DGVLNYPIYWQLLYAFES. . . SSGSISNLYNMI
Template Known Secondary structure  .......G....TS



S
GGGGGTS....SB
SS...TTS
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   215....220... ......230.........240...... ...250.........260.... .....270......
 
   305....310.........320.........330.........340.........350.........360.........370.........380....
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Query Sequence  RHWQQGMHNVAWNALFWCNHDQPRIVSRFGDEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVES
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Template Sequence  KSVASDCSDPTLLGNFIENHDNPRFAKYTSD. . . . . YSQAKNVLSYIFLSDGIPIVYAGEEQHYAGGKVPY. . . . . . . . .
Template Known Secondary structure 
S
GGGS
S

TTS

GGGT


.....SSSTTTTTT



TTTT.........
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   277..280.........290.........300....... ..310.........320.........330.........340..
 
   385....390.........400.........410.........420.........430.........440.........450.........460....
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Query Sequence  LNMFAELRNDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALADDSSVFYTYQKLIA
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . NREATWLS. . . . . . . . . . . . . . . . . . . . . . . . . . . GYDTSAELYTWIATTNA
Template Known Secondary structure  ............................T


GGGG...........................TT
TT
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   343......350 .........360.......
 
   465....470........ .480.........490....... ..500.........510.........520 ....... ..530..
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Query Sequence  LRKQEAILTWGNYQ. . . DLLPNSPVLWCYRREWKGQ. TLLVIANLSREIQPWQAGQMRGN. . . . . . WQLVMHN. . YEEAS
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Template Sequence  IRKLAIAADSAYITYANDAFYTDSNTIAMAKGTSGSQVITVLSNKGSSGSSYTLTLSGSGYTSGTKLIEAYTCTSVTVDS
Template Known Secondary structure 
TTTTTS

TTSSTTT

S
TT








TT
TTTT

T
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   368.370.........380.........390.........400.........410.........420.........430.........440.......
 
   533......540.........550.
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Query Sequence  PQPCAMNLRPFEAVWWLQK
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Template Sequence  SGDIPVPMASGLPRVLLPA
Template Known Secondary structure  TS

TT

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   448.450.........460......
 
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No model constructed - rank, confidence too low




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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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