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Job DescriptionP76223
Confidence100.00%DateThu Jan 5 12:20:50 GMT 2012
Rank1Aligned Residues340
% Identity15%Templatec3osqA_
PDB info PDB header:fluorescent protein, transport proteinChain: A: PDB Molecule:maltose-binding periplasmic protein, green fluorescent PDBTitle: maltose-bound maltose sensor engineered by insertion of circularly2 permuted green fluorescent protein into e. coli maltose binding3 protein at position 175
Resolution1.90 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   31........40.........50.........60.........70.........80.........90.........100.........110
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Query Sequence  KGQTVWFNAWGGDTAINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGENFRTLKEA
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Template Sequence  EEGKLVI. . WINGDKGYNGLAEVGKKFEKDTGIKVTVEH. . . PDKLEEKFPQVAATGD. . . . GPDIIFWAHDRFGGYAQS
Template Known Secondary structure 
TTS..

TTS




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TTTT
....S
SGGGT
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   3...... 10.........20.........30......... 40.........50..... ....60.........70...
 
   111........120.........130.........140.........150.........160.........170.........180.........190
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Query Sequence  NLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPD
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Template Sequence  GLLAEITP. . . DKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFN
Template Known Secondary structure  T
B




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TTB
TTT
S

TTT
SS

SBSTTTTT



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   74.....80. ........90.........100.........110.........120.........130.........140.........150
 
   191........200.........210.....
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Query Sequence  FTGTAFLEQLLIMLTPDPAALKEAP. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  LQEPYFTWPLIAADGGYAFKYENHLSHNVYIMADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYL
Template Known Secondary structure 
SSTT

S


GGGTBTTS
SSSS





S
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   151........160.........170.........180.........190.........200.........210.........220.........230
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation                                                                                  
Template Sequence  STQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
Template Known Secondary structure 

TT

SS




TT


TGGGGGSSTTTTTT
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   231........240.........250.........260.........270.........280.........290.........300.........310
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation                                                                                  
Template Sequence  TLKFICTTGKLPVPWPTLVTTLVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
Template Known Secondary structure  TTSS
SS
GGGGTTT

GGG
B

GGGGGG
TTTT
TTS
TT
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   317..320.........330.........340.........350.........360.........370.........380.........390......
 
   216...220.........230.........240.........250.........260.........270
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . DDATFARVTAPLWQYLDVLHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPA
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Template Sequence  KGIDFKEDGNILGHKLEYNFNGGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWA
Template Known Secondary structure  S

TTSTTTTT
B
SSSTTTTSSSTTSS
TT

TTS
GGG
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   400.........410.........420.........430.........440.........450.........460.........470.........
 
   271........280.........290.. .......300.........310.........320.........330.........340........
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Query Sequence  HAQQKIASGDLPASSYSFGFRE. . GMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAVWGDPSVLDPQKLPD
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Template Sequence  WSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP. LGAVALKSYEEE
Template Known Secondary structure  T




BBTTB



BTT
S
TTSSS
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SB
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   480.........490.........500.........510.........520.........530.........540...... ...550........
 
   349350.........360.........370 .........380...
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Query Sequence  GQRESLQSRMPQDLPPVLAEPH. . . . AGWVNALEQEWLH
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Template Sequence  LVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
Template Known Secondary structure  TTSS



TT
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   559560.........570.........580.........590.......
 
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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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