Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	K	I	I	P	G	A	T	G	Y	F	N	K	T	L	N	S	N	Q	F	D	N	E	D	A	I	K	D	K	L	D	N	R	G	S	I	K	G	K	L	N	N	I	Y	G	K	S	I	D	Y	A	A	L	R	H	R	D	I	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?										?	?	?	?	?	?	?								?	?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.
Sequence		I	A	K	I	D	L	F	I	Q	R	I	T	H	N	L	W	H	A	R	K	K	M	C	F
Secondary structure
SS confidence
Disorder																						?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2zxe chain G Region: 50 - 71 Aligned: 21 Modelled: 22 Confidence: 21.1% Identity: 38% PDB header:hydrolase/transport protein Chain: G: PDB Molecule:phospholemman-like protein; PDBTitle: crystal structure of the sodium - potassium pump in the e2.2k+.pi2 state Phyre2 PDB 3n23 chain E Region: 50 - 72 Aligned: 22 Modelled: 23 Confidence: 15.2% Identity: 32% PDB header:hydrolase Chain: E: PDB Molecule:na+/k+ atpase gamma subunit transcript variant a; PDBTitle: crystal structure of the high affinity complex between ouabain and the2 e2p form of the sodium-potassium pump Phyre2 PDB 3vub chain A Region: 55 - 71 Aligned: 17 Modelled: 17 Confidence: 12.8% Identity: 24% Fold: SH3-like barrel Superfamily: Cell growth inhibitor/plasmid maintenance toxic component Family: CcdB Phyre2 PDB 3jrz chain A Region: 56 - 71 Aligned: 16 Modelled: 16 Confidence: 9.9% Identity: 50% PDB header:toxin Chain: A: PDB Molecule:ccdb; PDBTitle: ccdbvfi-formii-ph5.6 Phyre2 PDB 2jo1 chain A Region: 50 - 72 Aligned: 22 Modelled: 23 Confidence: 9.0% Identity: 45% PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles Phyre2 PDB 2jp3 chain A Region: 50 - 71 Aligned: 21 Modelled: 22 Confidence: 8.5% Identity: 29% PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles Phyre2 PDB 2l3y chain A Region: 65 - 83 Aligned: 19 Modelled: 19 Confidence: 7.3% Identity: 42% PDB header:transcription Chain: A: PDB Molecule:interleukin-6; PDBTitle: solution structure of mouse il-6 Phyre2 PDB 2gv8 chain A domain 2 Region: 5 - 30 Aligned: 26 Modelled: 26 Confidence: 7.0% Identity: 23% Fold: FAD/NAD(P)-binding domain Superfamily: FAD/NAD(P)-binding domain Family: FAD/NAD-linked reductases, N-terminal and central domains Phyre2 PDB 1ail chain A Region: 75 - 82 Aligned: 8 Modelled: 8 Confidence: 6.1% Identity: 63% Fold: S15/NS1 RNA-binding domain Superfamily: S15/NS1 RNA-binding domain Family: N-terminal, RNA-binding domain of nonstructural protein NS1 Phyre2 PDB 3lfk chain C Region: 33 - 54 Aligned: 22 Modelled: 22 Confidence: 5.8% Identity: 50% PDB header:unknown function Chain: C: PDB Molecule:marr like protein, tvg0766549; PDBTitle: a reported archaeal mechanosensitive channel is a structural2 homolog of marr-like transcriptional regulators Phyre2 1 c2zxeG_ 2 c3n23E_ 3 d3vuba_ 4 c3jrzA_ 5 c2jo1A_ 6 c2jp3A_ 7 c2l3yA_ 8 d2gv8a2 9 d1aila_ 10 c3lfkC_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2zxeG_ 21.1 38 PDB header:hydrolase/transport protein Chain: G: PDB Molecule:phospholemman-like protein; PDBTitle: crystal structure of the sodium - potassium pump in the e2.2k+.pi2 state 2 c3n23E_ 15.2 32 PDB header:hydrolase Chain: E: PDB Molecule:na+/k+ atpase gamma subunit transcript variant a; PDBTitle: crystal structure of the high affinity complex between ouabain and the2 e2p form of the sodium-potassium pump 3 d3vuba_ 12.8 24 Fold:SH3-like barrel Superfamily:Cell growth inhibitor/plasmid maintenance toxic component Family:CcdB 4 c3jrzA_ 9.9 50 PDB header:toxin Chain: A: PDB Molecule:ccdb; PDBTitle: ccdbvfi-formii-ph5.6 5 c2jo1A_ 9.0 45 PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles 6 c2jp3A_ 8.5 29 PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles 7 c2l3yA_ 7.3 42 PDB header:transcription Chain: A: PDB Molecule:interleukin-6; PDBTitle: solution structure of mouse il-6 8 d2gv8a2 7.0 23 Fold:FAD/NAD(P)-binding domain Superfamily:FAD/NAD(P)-binding domain Family:FAD/NAD-linked reductases, N-terminal and central domains 9 d1aila_ 6.1 63 Fold:S15/NS1 RNA-binding domain Superfamily:S15/NS1 RNA-binding domain Family:N-terminal, RNA-binding domain of nonstructural protein NS1 10 c3lfkC_ 5.8 50 PDB header:unknown function Chain: C: PDB Molecule:marr like protein, tvg0766549; PDBTitle: a reported archaeal mechanosensitive channel is a structural2 homolog of marr-like transcriptional regulators

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0