Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	K	R	I	I	W	I	L	F	L	L	G	L	T	W	G	C	E	L	F	A	H	D	G	T	V	N	I	S	G	S	F	R	R	N	T	C	V	L	A	Q	D	S	K	Q	I	N	V	Q	L	G	D	V	S	L	T	R	F	S
Secondary structure
SS confidence
Disorder		?	?															?	?	?	?	?	?																			?	?	?	?	?															?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		H	G	N	Y	G	P	E	K	S	F	I	I	N	L	Q	D	C	G	T	D	V	S	T	V	D	V	T	F	S	G	T	P	D	G	V	Q	S	E	M	L	S	I	E	S	G	T	D	A	A	S	G	L	A	I	A	I	L	D	D	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?											?		?										?		?	?	?	?	?						?	?			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.
Sequence		K	I	L	I	P	L	N	Q	A	S	K	D	Y	S	L	H	S	G	K	V	P	L	T	F	Y	A	Q	L	R	P	V	N	S	D	V	Q	S	G	K	V	N	A	S	A	T	F	V	L	H	Y	D
Secondary structure
SS confidence
Disorder			?			?			?	?	?	?	?			?	?															?		?		?															?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3jwn chain L Region: 23 - 171 Aligned: 149 Modelled: 149 Confidence: 100.0% Identity: 36% PDB header:protein binding/cell adhesion Chain: L: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh Phyre2 PDB 3jwn chain K Region: 23 - 171 Aligned: 149 Modelled: 149 Confidence: 100.0% Identity: 36% PDB header:protein binding/cell adhesion Chain: K: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh Phyre2 PDB 3jwn chain E Region: 23 - 171 Aligned: 149 Modelled: 149 Confidence: 100.0% Identity: 36% PDB header:protein binding/cell adhesion Chain: E: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh Phyre2 PDB 3jwn chain F Region: 23 - 171 Aligned: 149 Modelled: 149 Confidence: 99.9% Identity: 36% PDB header:protein binding/cell adhesion Chain: F: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh Phyre2 PDB 2jmr chain A Region: 23 - 171 Aligned: 149 Modelled: 149 Confidence: 99.9% Identity: 37% PDB header:cell adhesion Chain: A: PDB Molecule:fimf; PDBTitle: nmr structure of the e. coli type 1 pilus subunit fimf Phyre2 PDB 2j2z chain B domain 1 Region: 26 - 171 Aligned: 142 Modelled: 146 Confidence: 99.9% Identity: 20% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 2jty chain A Region: 18 - 171 Aligned: 153 Modelled: 154 Confidence: 99.9% Identity: 27% PDB header:structural protein Chain: A: PDB Molecule:type-1 fimbrial protein, a chain; PDBTitle: self-complemented variant of fima, the main subunit of type 1 pilus Phyre2 PDB 2uy6 chain B domain 1 Region: 26 - 171 Aligned: 142 Modelled: 146 Confidence: 99.9% Identity: 24% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 3bfw chain A Region: 35 - 171 Aligned: 130 Modelled: 137 Confidence: 99.9% Identity: 18% PDB header:structural protein/structural protein Chain: A: PDB Molecule:protein fimg; PDBTitle: crystal structure of truncated fimg (fimgt) in complex with the donor2 strand peptide of fimf (dsf) Phyre2 PDB 1pdk chain B Region: 33 - 171 Aligned: 139 Modelled: 139 Confidence: 99.9% Identity: 22% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 3bwu chain F Region: 35 - 171 Aligned: 124 Modelled: 137 Confidence: 99.8% Identity: 37% PDB header:chaperone, structural, membrane protein Chain: F: PDB Molecule:protein fimf; PDBTitle: crystal structure of the ternary complex of fimd (n-terminal domain,2 fimdn) with fimc and the n-terminally truncated pilus subunit fimf3 (fimft) Phyre2 PDB 1ze3 chain H domain 1 Region: 36 - 171 Aligned: 121 Modelled: 136 Confidence: 99.8% Identity: 16% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 1klf chain P Region: 27 - 171 Aligned: 132 Modelled: 145 Confidence: 99.8% Identity: 14% PDB header:chaperone/adhesin complex Chain: P: PDB Molecule:fimh protein; PDBTitle: fimh adhesin-fimc chaperone complex with d-mannose Phyre2 PDB 2w07 chain B Region: 32 - 171 Aligned: 120 Modelled: 140 Confidence: 99.8% Identity: 15% PDB header:cell adhesion Chain: B: PDB Molecule:minor pilin subunit papf; PDBTitle: structural determinants of polymerization reactivity of the2 p pilus adaptor subunit papf Phyre2 PDB 1n12 chain A Region: 36 - 171 Aligned: 129 Modelled: 136 Confidence: 99.5% Identity: 20% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 2wmp chain B Region: 36 - 170 Aligned: 117 Modelled: 123 Confidence: 61.8% Identity: 17% PDB header:chaperone Chain: B: PDB Molecule:papg protein; PDBTitle: structure of the e. coli chaperone papd in complex with the pilin2 domain of the papgii adhesin Phyre2 PDB 2jna chain A domain 1 Region: 1 - 26 Aligned: 26 Modelled: 26 Confidence: 7.5% Identity: 31% Fold: Dodecin subunit-like Superfamily: YdgH-like Family: YdgH-like Phyre2 PDB 1z1z chain A domain 1 Region: 160 - 171 Aligned: 12 Modelled: 12 Confidence: 5.2% Identity: 33% Fold: Phage tail protein-like Superfamily: Phage tail protein-like Family: Lambda phage gpU-like Phyre2 PDB 1dm9 chain A Region: 4 - 53 Aligned: 49 Modelled: 50 Confidence: 5.1% Identity: 10% PDB header:structural genomics Chain: A: PDB Molecule:hypothetical 15.5 kd protein in mrca-pcka PDBTitle: heat shock protein 15 kd Phyre2 1 c3jwnL_ 2 c3jwnK_ 3 c3jwnE_ 4 c3jwnF_ 5 c2jmrA_ 6 d2j2zb1 7 c2jtyA_ 8 d2uy6b1 9 c3bfwA_ 10 d1pdkb_ 11 c3bwuF_ 12 d1ze3h1 13 c1klfP_ 14 c2w07B_ 15 d1n12a_ 16 c2wmpB_ 17 d2jnaa1 18 d1z1za1 19 c1dm9A_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3jwnL_ 100.0 36 PDB header:protein binding/cell adhesion Chain: L: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh 2 c3jwnK_ 100.0 36 PDB header:protein binding/cell adhesion Chain: K: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh 3 c3jwnE_ 100.0 36 PDB header:protein binding/cell adhesion Chain: E: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh 4 c3jwnF_ 99.9 36 PDB header:protein binding/cell adhesion Chain: F: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh 5 c2jmrA_ 99.9 37 PDB header:cell adhesion Chain: A: PDB Molecule:fimf; PDBTitle: nmr structure of the e. coli type 1 pilus subunit fimf 6 d2j2zb1 99.9 20 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 7 c2jtyA_ 99.9 27 PDB header:structural protein Chain: A: PDB Molecule:type-1 fimbrial protein, a chain; PDBTitle: self-complemented variant of fima, the main subunit of type 1 pilus 8 d2uy6b1 99.9 24 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 9 c3bfwA_ 99.9 18 PDB header:structural protein/structural protein Chain: A: PDB Molecule:protein fimg; PDBTitle: crystal structure of truncated fimg (fimgt) in complex with the donor2 strand peptide of fimf (dsf) 10 d1pdkb_ 99.9 22 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 11 c3bwuF_ 99.8 37 PDB header:chaperone, structural, membrane protein Chain: F: PDB Molecule:protein fimf; PDBTitle: crystal structure of the ternary complex of fimd (n-terminal domain,2 fimdn) with fimc and the n-terminally truncated pilus subunit fimf3 (fimft) 12 d1ze3h1 99.8 16 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 13 c1klfP_ 99.8 14 PDB header:chaperone/adhesin complex Chain: P: PDB Molecule:fimh protein; PDBTitle: fimh adhesin-fimc chaperone complex with d-mannose 14 c2w07B_ 99.8 15 PDB header:cell adhesion Chain: B: PDB Molecule:minor pilin subunit papf; PDBTitle: structural determinants of polymerization reactivity of the2 p pilus adaptor subunit papf 15 d1n12a_ 99.5 20 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 16 c2wmpB_ 61.8 17 PDB header:chaperone Chain: B: PDB Molecule:papg protein; PDBTitle: structure of the e. coli chaperone papd in complex with the pilin2 domain of the papgii adhesin 17 d2jnaa1 7.5 31 Fold:Dodecin subunit-like Superfamily:YdgH-like Family:YdgH-like 18 d1z1za1 5.2 33 Fold:Phage tail protein-like Superfamily:Phage tail protein-like Family:Lambda phage gpU-like 19 c1dm9A_ 5.1 10 PDB header:structural genomics Chain: A: PDB Molecule:hypothetical 15.5 kd protein in mrca-pcka PDBTitle: heat shock protein 15 kd

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0