Job Description P77432 Confidence 100.00% Date Thu Jan 5 12:29:13 GMT 2012 Rank 3 Aligned Residues 481 % Identity 24% Template c3flcX_ PDB info PDB header:transferase Chain: X: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of the his-tagged h232r mutant of glycerol kinase2 from enterococcus casseliflavus with glycerol Resolution 1.85 Å

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		10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		S	K	Y	Y	L	M	A	L	D	A	G	T	G	S	I	R	A	V	I	F	D	L	E	G	N	Q	I	A	V	G	Q	A	E	W	R	H	L	A	V	P	D	V	P	G	S	M	E	F	D	L	N	K	N	W	Q	L	A	C	E	C	M	R	Q	A	L	H	N	A	G	I	A	P	E	Y	I	A	A	V	S	A
Query Conservation
Alig confidence																																								.	.
Template Conservation																																								.	.
Template Sequence		E	K	N	Y	V	M	A	I	D	Q	G	T	T	S	S	R	A	I	I	F	D	R	N	G	K	K	I	G	S	S	Q	K	E	F	P	Q	Y	F	.	.	P	K	S	G	W	V	E	H	N	A	N	E	I	W	N	S	V	Q	S	V	I	A	G	A	F	I	E	S	G	I	R	P	E	A	I	A	G	I	G	I
Template Known Secondary structure													S	S									T	T	S													B		.	.	S	S	T	T																												G	G	G
Template Predicted Secondary structure																																								.	.
Template SS confidence
		3	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40			.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80
		90	.	.	.	.	.	.	.	.	.	100	.		.	.	.	.	.	.	.		.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140		.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.
Predicted Secondary structure														.								.																																	.
Query SS confidence														.								.																																	.
Query Sequence		C	S	M	R	E	G	I	V	L	Y	N	N	.	E	G	A	P	I	W	A	.	C	A	N	V	D	A	R	A	A	R	E	V	S	E	L	K	E	L	H	N	N	T	F	E	N	E	V	Y	R	A	T	G	.	Q	T	L	A	L	S	A	I	P	R	L	L	W	L	A	H	H	R	S	D	I	Y	R	Q	A	S
Query Conservation														.								.																																	.
Alig confidence														.								.																					.	.	.										.
Template Conservation																																											.	.	.
Template Sequence		T	N	Q	R	E	T	T	V	V	W	D	K	T	T	G	Q	P	I	A	N	A	I	V	W	Q	S	R	Q	S	S	P	I	A	D	Q	L	K	V	D	G	H	.	.	.	T	E	M	I	H	E	K	T	G	L	V	I	D	A	Y	F	S	A	T	K	V	R	W	L	L	D	N	I	E	G	A	Q	E	K	A	D
Template Known Secondary structure					S	S							T	T	T			B	S	S					T	T			T	T										T	T		.	.	.									S					T	T	S												S	T	T
Template Predicted Secondary structure																																											.	.	.
Template SS confidence
		81	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.				.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.
		167					.	.	170	.	.	.	.	.	.	.	.	.	180	.	.			.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Predicted Secondary structure			.	.	.	.																.	.
Query SS confidence			.	.	.	.																.	.
Query Sequence		T	.	.	.	.	I	T	M	I	S	D	W	L	A	Y	M	L	S	G	E	.	.	L	A	V	D	P	S	N	A	G	T	T	G	L	L	D	L	T	T	R	D	W	K	P	A	L	L	D	M	A	G	L	R	A	D	I	L	S	P	V	K	E	T	G	T	L	L	G	V	V	S	S	Q	A	A	E	L	C	G
Query Conservation			.	.	.	.																.	.
Alig confidence			.	.	.	.																.	.																																																					.	.	.	.
Template Conservation																																																																												.	.	.	.
Template Sequence		N	G	E	L	L	F	G	T	I	D	S	W	L	V	W	K	L	T	D	G	Q	V	H	V	T	D	Y	S	N	A	S	R	T	M	L	Y	N	I	H	K	L	E	W	D	Q	E	I	L	D	L	L	N	I	P	S	S	M	L	P	E	V	K	S	N	S	E	V	Y	G	H	T	R	S	Y	.	.	.	.	R	F
Template Known Secondary structure		T	T																T	T	T	S											T	T	T	S			T	T	T	T										T	T			G	G	G							S									G	G	.	.	.	.	G	G
Template Predicted Secondary structure																																																																												.	.	.	.
Template SS confidence
		158	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.					.	.
		241	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.			.	.	.	.	.	310	.	.	.	.	.	.	.	.
Predicted Secondary structure																																																																		.	.
Query SS confidence																																																																		.	.
Query Sequence		L	K	A	G	T	P	V	V	V	G	G	G	D	V	Q	L	G	C	L	G	L	G	V	V	R	P	A	Q	T	A	V	L	G	G	T	F	W	Q	Q	V	V	N	L	A	A	P	V	T	D	P	E	M	N	V	R	V	N	P	H	V	I	P	G	M	.	.	V	Q	A	E	S	I	S	F	F	T	G	L	T	M
Query Conservation																																																																		.	.
Alig confidence																																																																		.	.
Template Conservation
Template Sequence		Y	G	S	E	V	P	I	A	G	M	A	G	D	Q	Q	A	A	L	F	G	Q	M	A	F	E	K	G	M	I	K	N	T	Y	G	T	G	A	F	I	V	M	N	T	G	E	E	P	L	S	D	N	D	L	L	T	T	I	G	Y	G	I	N	G	K	V	Y	Y	A	L	E	G	S	I	F	V	A	G	S	A	I
Template Known Secondary structure		T	T																			T	T			S	T	T							S	S	S									T						T	T	S									T	T											S		S
Template Predicted Secondary structure
Template SS confidence
		234	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.
		319	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.			.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.
Predicted Secondary structure																																									.	.
Query SS confidence																																									.	.
Query Sequence		R	W	F	R	D	A	F	C	A	E	E	K	L	I	A	E	R	L	G	I	D	T	Y	T	L	L	E	E	M	A	S	R	V	P	P	G	S	W	G	.	.	V	M	P	I	F	S	D	R	M	R	F	K	T	W	Y	H	A	A	P	S	F	I	N	L	S	I	D	P	D	K	C	N	K	A	T	L	F	R	A
Query Conservation																																									.	.
Alig confidence												.	.	.	.	.	.	.	.	.	.	.																			.	.																												.	.	.
Template Conservation												.	.	.	.	.	.	.	.	.	.	.																																																.	.	.
Template Sequence		Q	W	L	R	D	G	L	R	M	I	.	.	.	.	.	.	.	.	.	.	.	E	T	S	P	Q	S	E	E	L	A	A	K	A	K	G	D	N	E	V	Y	V	V	P	A	F	T	G	L	G	A	P	Y	W	D	S	E	A	R	G	A	V	F	G	L	T	R	G	.	.	.	T	T	K	E	D	F	V	R	A
Template Known Secondary structure							T	S			S	.	.	.	.	.	.	.	.	.	.	.	S	S	T	T											S		S								T	T		B		T	T	T		B	T	T										T	T	.	.	.
Template Predicted Secondary structure												.	.	.	.	.	.	.	.	.	.	.																																																.	.	.
Template SS confidence
		315	.	.	.	.	320	.	.	.	.												.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.				.	.	.	.	.	.	.	.	380
		397	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		L	E	E	N	A	A	I	V	S	A	C	N	L	Q	Q	I	A	D	F	S	N	I	H	P	S	S	L	V	F	A	G	G	G	S	K	G	K	L	W	S	Q	I	L	A	D	V	S	G	L	P	V	N	I	P	V	V	K	E	A	T	A	L	G	C	A	I	A	A	G	V	G	A	G	I	F	S	S	M	A	E
Query Conservation
Alig confidence
Template Conservation
Template Sequence		T	L	Q	A	V	A	Y	Q	S	K	D	V	I	D	T	M	K	K	D	S	G	I	D	I	P	L	L	K	V	D	G	G	A	A	K	N	D	L	L	M	Q	F	Q	A	D	I	L	D	I	D	V	Q	R	A	A	N	L	E	T	T	A	L	G	A	A	Y	L	A	G	L	A	V	G	F	W	K	D	L	D	E
Template Known Secondary structure																																S	G	G	G	G													T	S						S	S	S															T	T	S	S
Template Predicted Secondary structure
Template SS confidence
		381	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460
		477	.	.	480	.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		T	G	E	R	L	V	R	W	E	R	T	H	T	P	D	P	E	K	H	E	L	Y	Q	D	S	R	D	K	W	Q	A	V	Y	Q	D	Q	L	G	L
Query Conservation
Alig confidence			.																			.
Template Conservation			.																			.
Template Sequence		L	.	K	S	M	A	E	E	G	Q	M	F	T	P	E	M	P	A	E	E	.	R	D	N	L	Y	E	G	W	K	Q	A	V	A	A	T	Q	T	F
Template Known Secondary structure			.			T											S					.
Template Predicted Secondary structure			.																			.
Template SS confidence
		461		.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.		480	.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.