Job Description P77432 Confidence 100.00% Date Thu Jan 5 12:29:13 GMT 2012 Rank 13 Aligned Residues 470 % Identity 25% Template c3ezwD_ PDB info PDB header:transferase Chain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices Resolution 2.00 Å

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		10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		S	K	Y	Y	L	M	A	L	D	A	G	T	G	S	I	R	A	V	I	F	D	L	E	G	N	Q	I	A	V	G	Q	A	E	W	R	H	L	A	V	P	D	V	P	G	S	M	E	F	D	L	N	K	N	W	Q	L	A	C	E	C	M	R	Q	A	L	H	N	A	G	I	A	P	E	Y	I	A	A	V	S	A
Query Conservation
Alig confidence																																								.	.
Template Conservation																																								.	.
Template Sequence		E	K	K	Y	I	V	A	L	D	Q	G	T	T	S	S	R	A	V	V	M	D	H	D	A	N	I	I	S	V	S	Q	R	E	F	E	Q	I	Y	.	.	P	K	P	G	W	V	E	H	D	P	M	E	I	W	A	T	Q	S	S	T	L	V	E	V	L	A	K	A	D	I	S	S	D	Q	I	A	A	I	G	I
Template Known Secondary structure													S	S									T	T														B		.	.		S	T	T																								T	T			G	G	G
Template Predicted Secondary structure																																								.	.
Template SS confidence
		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.			40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.
		90	.	.	.	.	.	.	.	.	.	100	.		.	.	.	.	.	.	.		.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140		.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.
Predicted Secondary structure														.								.																																	.
Query SS confidence														.								.																																	.
Query Sequence		C	S	M	R	E	G	I	V	L	Y	N	N	.	E	G	A	P	I	W	A	.	C	A	N	V	D	A	R	A	A	R	E	V	S	E	L	K	E	L	H	N	N	T	F	E	N	E	V	Y	R	A	T	G	.	Q	T	L	A	L	S	A	I	P	R	L	L	W	L	A	H	H	R	S	D	I	Y	R	Q	A	S
Query Conservation														.								.																																	.
Alig confidence														.								.																					.	.	.										.
Template Conservation																																											.	.	.
Template Sequence		T	N	Q	R	E	T	T	I	V	W	E	K	E	T	G	K	P	I	Y	N	A	I	V	W	Q	C	R	R	T	A	E	I	C	E	H	L	K	R	D	G	L	.	.	.	E	D	Y	I	R	S	N	T	G	L	V	I	D	P	Y	F	S	G	T	K	V	K	W	I	L	D	H	V	E	G	S	R	E	R	A	R
Template Known Secondary structure					S	S		B					T	T	T			B	S	S					T	T			T	T										T	T		.	.	.									S			S	S	T	T	S												S	T	T
Template Predicted Secondary structure																																											.	.	.
Template SS confidence
		80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120				.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.
		167					.	.	170	.	.	.	.	.	.	.	.	.	180	.			.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Predicted Secondary structure			.	.	.	.															.	.
Query SS confidence			.	.	.	.															.	.
Query Sequence		T	.	.	.	.	I	T	M	I	S	D	W	L	A	Y	M	L	S	G	.	.	E	L	A	V	D	P	S	N	A	G	T	T	G	L	L	D	L	T	T	R	D	W	K	P	A	L	L	D	M	A	G	L	R	A	D	I	L	S	P	V	K	E	T	G	T	L	L	G	V	V	S	S	Q	A	A	E	L	C	G
Query Conservation			.	.	.	.															.	.
Alig confidence			.	.	.	.															.	.																																																				.	.	.	.	.	.	.	.
Template Conservation																																																																										.	.	.	.	.	.	.	.
Template Sequence		R	G	E	L	L	F	G	T	V	D	T	W	L	I	W	K	M	T	Q	G	R	V	H	V	T	D	Y	T	N	A	S	R	T	M	L	F	N	I	H	T	L	D	W	D	D	K	M	L	E	V	L	D	I	P	R	E	M	L	P	E	V	R	R	S	S	E	V	Y	G	Q	T	N	.	.	.	.	.	.	.	.
Template Known Secondary structure		T	T	S															T	T	T	S										G	G	G	S	S			T	T	T	T										T	T			G	G	G							S									.	.	.	.	.	.	.	.
Template Predicted Secondary structure																																																																										.	.	.	.	.	.	.	.
Template SS confidence
		157	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.
		241	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.				.	.	.	.	.	.	310	.	.	.	.	.	.	.
Predicted Secondary structure																																																																	.	.	.
Query SS confidence																																																																	.	.	.
Query Sequence		L	K	A	G	T	P	V	V	V	G	G	G	D	V	Q	L	G	C	L	G	L	G	V	V	R	P	A	Q	T	A	V	L	G	G	T	F	W	Q	Q	V	V	N	L	A	A	P	V	T	D	P	E	M	N	V	R	V	N	P	H	V	I	P	G	.	.	.	M	V	Q	A	E	S	I	S	F	F	T	G	L	T
Query Conservation																																																																	.	.	.
Alig confidence		.	.	.																																																													.	.	.
Template Conservation		.	.	.
Template Sequence		.	.	.	R	I	P	I	S	G	I	A	G	D	Q	Q	A	A	L	F	G	Q	L	C	V	K	E	G	M	A	K	N	T	Y	G	T	G	C	F	M	L	M	N	T	G	E	K	A	V	K	S	E	N	G	L	L	T	T	I	A	C	G	P	T	G	E	V	N	Y	A	L	E	G	A	V	F	M	A	G	A	S
Template Known Secondary structure		.	.	.																		T	T			S	T	T							S	S	S								T	T	S					S	S	S									T	T	S											S		S
Template Predicted Secondary structure		.	.	.
Template SS confidence
					236	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.
		318	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		M	R	W	F	R	D	A	F	C	A	E	E	K	L	I	A	E	R	L	G	I	D	T	Y	T	L	L	E	E	M	A	S	R	V	P	P	G	S	W	G	V	M	P	I	F	S	D	R	M	R	F	K	T	W	Y	H	A	A	P	S	F	I	N	L	S	I	D	P	D	K	C	N	K	A	T	L	F	R	A	L
Query Conservation
Alig confidence																		.	.	.	.	.	.	.	.	.	.	.	.	.																																							.	.	.
Template Conservation																		.	.	.	.	.	.	.	.	.	.	.	.	.																																							.	.	.
Template Sequence		I	Q	W	L	R	D	E	M	K	L	I	D	S	E	Y	F	.	.	.	.	.	.	.	.	.	.	.	.	.	A	T	K	V	Q	N	T	N	G	V	Y	V	V	P	A	F	T	G	L	G	A	P	Y	W	D	P	Y	A	R	G	A	I	F	G	L	T	R	G	.	.	.	V	N	A	N	H	I	I	R	A	T
Template Known Secondary structure								T	S									.	.	.	.	.	.	.	.	.	.	.	.	.			T	S	S	S		T	T							T	T		B		T	T	T		B	T	T										T	T	.	.	.
Template Predicted Secondary structure																		.	.	.	.	.	.	.	.	.	.	.	.	.																																							.	.	.
Template SS confidence
		313	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.														.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.				.	.	.	370	.	.	.	.	.	.
		398	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		E	E	N	A	A	I	V	S	A	C	N	L	Q	Q	I	A	D	F	S	N	I	H	P	S	S	L	V	F	A	G	G	G	S	K	G	K	L	W	S	Q	I	L	A	D	V	S	G	L	P	V	N	I	P	V	V	K	E	A	T	A	L	G	C	A	I	A	A	G	V	G	A	G	I	F	S	S	M	A	E	T
Query Conservation
Alig confidence																																																						.	.	.																									.
Template Conservation																																																						.	.	.																									.
Template Sequence		L	E	S	I	A	Y	Q	T	R	D	V	L	E	A	M	Q	A	D	S	G	I	R	L	H	A	L	R	V	D	G	G	A	V	A	N	N	F	L	M	Q	F	Q	S	D	I	L	G	T	R	V	E	R	.	.	.	P	E	V	T	A	L	G	A	A	Y	L	A	G	L	A	V	G	F	W	Q	N	L	D	E	.
Template Known Secondary structure																									S						S	G	G	G	G													T	S					.	.	.																	T	S	S	S					.
Template Predicted Secondary structure																																																						.	.	.																									.
Template SS confidence
		381	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.				.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.
		478	.	480	.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		G	E	R	L	V	R	W	E	R	T	H	T	P	D	P	E	K	H	E	L	Y	Q	D	S	R	D	K	W	Q	A	V	Y	Q	D	Q	L	G	L	V	D	H	G
Query Conservation
Alig confidence		.	.	.																					.
Template Conservation		.	.	.																					.
Template Sequence		.	.	.	L	Q	E	I	E	R	E	F	R	P	G	I	E	T	T	E	R	N	Y	R	.	Y	A	G	W	K	K	A	V	K	R	A	M	A	W	E	E	H	D
Template Known Secondary structure		.	.	.		T										S									.												S	S		S
Template Predicted Secondary structure		.	.	.																					.
Template SS confidence
					460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.		480	.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.