Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	I	Q	Q	W	L	F	S	F	K	G	R	I	G	R	R	D	F	W	I	W	I	G	L	W	F	A	G	M	L	V	L	F	S	L	A	G	K	N	L	L	D	I	Q	T	A	A	F	C	L	V	C	L	L	W	P	T	A	A
Secondary structure
SS confidence
Disorder		?	?																																			?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	T	V	K	R	L	H	D	R	G	R	S	G	A	W	A	F	L	M	I	V	A	W	M	L	L	A	G	N	W	A	I	L	P	G	V	W	Q	W	A	V	G	R	F	V	P	T	L	I	L	V	M	M	L	I	D	L	G	A	F
Secondary structure
SS confidence
Disorder																												?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.
Sequence		V	G	T	Q	G	E	N	K	Y	G	K	D	T	Q	D	V	K	Y	K	A	D	N	K	S	S	N
Secondary structure
SS confidence
Disorder						?	?	?	?	?					?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2axt chain I domain 1 Region: 102 - 127 Aligned: 26 Modelled: 26 Confidence: 16.1% Identity: 15% Fold: Single transmembrane helix Superfamily: Photosystem II reaction center protein I, PsbI Family: PsbI-like Phyre2 PDB 3gia chain A Region: 9 - 135 Aligned: 126 Modelled: 127 Confidence: 15.5% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter Phyre2 PDB 2dyo chain B Region: 61 - 70 Aligned: 10 Modelled: 10 Confidence: 12.8% Identity: 40% PDB header:protein turnover/protein turnover Chain: B: PDB Molecule:autophagy protein 16; PDBTitle: the crystal structure of saccharomyces cerevisiae atg5-2 atg16(1-57) complex Phyre2 PDB 1bzg chain A Region: 66 - 72 Aligned: 7 Modelled: 7 Confidence: 11.5% Identity: 71% PDB header:hormone Chain: A: PDB Molecule:parathyroid hormone-related protein; PDBTitle: the solution structure of human parathyroid hormone-related2 protein (1-34) in near-physiological solution, nmr, 303 structures Phyre2 PDB 3zrk chain Y Region: 62 - 67 Aligned: 6 Modelled: 6 Confidence: 11.2% Identity: 67% PDB header:transferase/peptide Chain: Y: PDB Molecule:proto-oncogene frat1; PDBTitle: identification of 2-(4-pyridyl)thienopyridinones as gsk-3beta2 inhibitors Phyre2 PDB 1gng chain X Region: 62 - 67 Aligned: 6 Modelled: 6 Confidence: 10.7% Identity: 67% PDB header:transferase Chain: X: PDB Molecule:frattide; PDBTitle: glycogen synthase kinase-3 beta (gsk3) complex with frattide2 peptide Phyre2 PDB 1lir chain A Region: 64 - 71 Aligned: 8 Modelled: 8 Confidence: 10.6% Identity: 63% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: Scorpion toxin-like Family: Short-chain scorpion toxins Phyre2 PDB 1tqe chain Y Region: 132 - 145 Aligned: 14 Modelled: 14 Confidence: 8.7% Identity: 7% PDB header:transcription/protein binding/dna Chain: Y: PDB Molecule:histone deacetylase 9; PDBTitle: mechanism of recruitment of class ii histone deacetylases2 by myocyte enhancer factor-2 Phyre2 PDB 1tqe chain X Region: 132 - 145 Aligned: 14 Modelled: 14 Confidence: 8.7% Identity: 7% PDB header:transcription/protein binding/dna Chain: X: PDB Molecule:histone deacetylase 9; PDBTitle: mechanism of recruitment of class ii histone deacetylases2 by myocyte enhancer factor-2 Phyre2 PDB 1zcz chain A Region: 128 - 135 Aligned: 8 Modelled: 8 Confidence: 8.5% Identity: 25% PDB header:transferase/hydrolase Chain: A: PDB Molecule:bifunctional purine biosynthesis protein purh; PDBTitle: crystal structure of phosphoribosylaminoimidazolecarboxamide2 formyltransferase / imp cyclohydrolase (tm1249) from thermotoga3 maritima at 1.88 a resolution Phyre2 PDB 1jwy chain A domain 1 Region: 129 - 140 Aligned: 12 Modelled: 12 Confidence: 8.4% Identity: 17% Fold: SH3-like barrel Superfamily: Myosin S1 fragment, N-terminal domain Family: Myosin S1 fragment, N-terminal domain Phyre2 PDB 3skd chain A Region: 66 - 71 Aligned: 6 Modelled: 6 Confidence: 7.5% Identity: 67% PDB header:hydrolase Chain: A: PDB Molecule:putative uncharacterized protein tthb187; PDBTitle: crystal structure of the thermus thermophilus cas3 hd domain in the2 presence of ni2+ Phyre2 PDB 3bz7 chain A domain 1 Region: 129 - 140 Aligned: 12 Modelled: 12 Confidence: 7.5% Identity: 17% Fold: SH3-like barrel Superfamily: Myosin S1 fragment, N-terminal domain Family: Myosin S1 fragment, N-terminal domain Phyre2 PDB 2eyq chain A domain 6 Region: 128 - 138 Aligned: 11 Modelled: 11 Confidence: 7.2% Identity: 9% Fold: TRCF domain-like Superfamily: TRCF domain-like Family: TRCF domain Phyre2 PDB 2ogi chain A Region: 66 - 71 Aligned: 6 Modelled: 6 Confidence: 6.7% Identity: 50% PDB header:hydrolase Chain: A: PDB Molecule:hypothetical protein sag1661; PDBTitle: crystal structure of a putative metal dependent phosphohydrolase2 (sag1661) from streptococcus agalactiae serogroup v at 1.85 a3 resolution Phyre2 PDB 2o08 chain B Region: 66 - 71 Aligned: 6 Modelled: 6 Confidence: 6.3% Identity: 33% PDB header:hydrolase Chain: B: PDB Molecule:bh1327 protein; PDBTitle: crystal structure of a putative hd superfamily hydrolase (bh1327) from2 bacillus halodurans at 1.90 a resolution Phyre2 PDB 3ccg chain A Region: 66 - 71 Aligned: 6 Modelled: 6 Confidence: 5.9% Identity: 33% PDB header:hydrolase Chain: A: PDB Molecule:hd superfamily hydrolase; PDBTitle: crystal structure of predicted hd superfamily hydrolase involved in2 nad metabolism (np_347894.1) from clostridium acetobutylicum at 1.503 a resolution Phyre2 PDB 2crd chain A Region: 64 - 71 Aligned: 8 Modelled: 8 Confidence: 5.8% Identity: 50% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: Scorpion toxin-like Family: Short-chain scorpion toxins Phyre2 PDB 2qsr chain A Region: 128 - 138 Aligned: 11 Modelled: 11 Confidence: 5.8% Identity: 27% PDB header:transcription Chain: A: PDB Molecule:transcription-repair coupling factor; PDBTitle: crystal structure of c-terminal domain of transcription-repair2 coupling factor Phyre2 PDB 4a1o chain B Region: 128 - 146 Aligned: 19 Modelled: 19 Confidence: 5.7% Identity: 16% PDB header:transferase-hydrolase Chain: B: PDB Molecule:bifunctional purine biosynthesis protein purh; PDBTitle: crystal structure of mycobacterium tuberculosis purh complexed with2 aicar and a novel nucleotide cfair, at 2.48 a resolution. Phyre2 1 d2axti1 2 c3giaA_ 3 c2dyoB_ 4 c1bzgA_ 5 c3zrkY_ 6 c1gngX_ 7 d1lira_ 8 c1tqeY_ 9 c1tqeX_ 10 c1zczA_ 11 d1jwya1 12 c3skdA_ 13 d3bz7a1 14 d2eyqa6 15 c2ogiA_ 16 c2o08B_ 17 c3ccgA_ 18 d2crda_ 19 c2qsrA_ 20 c4a1oB_ 21 22 23 24

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2axti1 16.1 15 Fold:Single transmembrane helix Superfamily:Photosystem II reaction center protein I, PsbI Family:PsbI-like 2 c3giaA_ 15.5 13 PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter 3 c2dyoB_ 12.8 40 PDB header:protein turnover/protein turnover Chain: B: PDB Molecule:autophagy protein 16; PDBTitle: the crystal structure of saccharomyces cerevisiae atg5-2 atg16(1-57) complex 4 c1bzgA_ 11.5 71 PDB header:hormone Chain: A: PDB Molecule:parathyroid hormone-related protein; PDBTitle: the solution structure of human parathyroid hormone-related2 protein (1-34) in near-physiological solution, nmr, 303 structures 5 c3zrkY_ 11.2 67 PDB header:transferase/peptide Chain: Y: PDB Molecule:proto-oncogene frat1; PDBTitle: identification of 2-(4-pyridyl)thienopyridinones as gsk-3beta2 inhibitors 6 c1gngX_ 10.7 67 PDB header:transferase Chain: X: PDB Molecule:frattide; PDBTitle: glycogen synthase kinase-3 beta (gsk3) complex with frattide2 peptide 7 d1lira_ 10.6 63 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Scorpion toxin-like Family:Short-chain scorpion toxins 8 c1tqeY_ 8.7 7 PDB header:transcription/protein binding/dna Chain: Y: PDB Molecule:histone deacetylase 9; PDBTitle: mechanism of recruitment of class ii histone deacetylases2 by myocyte enhancer factor-2 9 c1tqeX_ 8.7 7 PDB header:transcription/protein binding/dna Chain: X: PDB Molecule:histone deacetylase 9; PDBTitle: mechanism of recruitment of class ii histone deacetylases2 by myocyte enhancer factor-2 10 c1zczA_ 8.5 25 PDB header:transferase/hydrolase Chain: A: PDB Molecule:bifunctional purine biosynthesis protein purh; PDBTitle: crystal structure of phosphoribosylaminoimidazolecarboxamide2 formyltransferase / imp cyclohydrolase (tm1249) from thermotoga3 maritima at 1.88 a resolution 11 d1jwya1 8.4 17 Fold:SH3-like barrel Superfamily:Myosin S1 fragment, N-terminal domain Family:Myosin S1 fragment, N-terminal domain 12 c3skdA_ 7.5 67 PDB header:hydrolase Chain: A: PDB Molecule:putative uncharacterized protein tthb187; PDBTitle: crystal structure of the thermus thermophilus cas3 hd domain in the2 presence of ni2+ 13 d3bz7a1 7.5 17 Fold:SH3-like barrel Superfamily:Myosin S1 fragment, N-terminal domain Family:Myosin S1 fragment, N-terminal domain 14 d2eyqa6 7.2 9 Fold:TRCF domain-like Superfamily:TRCF domain-like Family:TRCF domain 15 c2ogiA_ 6.7 50 PDB header:hydrolase Chain: A: PDB Molecule:hypothetical protein sag1661; PDBTitle: crystal structure of a putative metal dependent phosphohydrolase2 (sag1661) from streptococcus agalactiae serogroup v at 1.85 a3 resolution 16 c2o08B_ 6.3 33 PDB header:hydrolase Chain: B: PDB Molecule:bh1327 protein; PDBTitle: crystal structure of a putative hd superfamily hydrolase (bh1327) from2 bacillus halodurans at 1.90 a resolution 17 c3ccgA_ 5.9 33 PDB header:hydrolase Chain: A: PDB Molecule:hd superfamily hydrolase; PDBTitle: crystal structure of predicted hd superfamily hydrolase involved in2 nad metabolism (np_347894.1) from clostridium acetobutylicum at 1.503 a resolution 18 d2crda_ 5.8 50 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Scorpion toxin-like Family:Short-chain scorpion toxins 19 c2qsrA_ 5.8 27 PDB header:transcription Chain: A: PDB Molecule:transcription-repair coupling factor; PDBTitle: crystal structure of c-terminal domain of transcription-repair2 coupling factor 20 c4a1oB_ 5.7 16 PDB header:transferase-hydrolase Chain: B: PDB Molecule:bifunctional purine biosynthesis protein purh; PDBTitle: crystal structure of mycobacterium tuberculosis purh complexed with2 aicar and a novel nucleotide cfair, at 2.48 a resolution. 21 c3rf1B_ not modelled 5.6 17 PDB header:ligase Chain: B: PDB Molecule:glycyl-trna synthetase alpha subunit; PDBTitle: the crystal structure of glycyl-trna synthetase subunit alpha from2 campylobacter jejuni subsp. jejuni nctc 11168 22 c2l2tA_ not modelled 5.5 19 PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-4; PDBTitle: solution nmr structure of the erbb4 dimeric membrane domain 23 c2levA_ not modelled 5.5 19 PDB header:transcription regulator/dna Chain: A: PDB Molecule:ler; PDBTitle: structure of the dna complex of the c-terminal domain of ler 24 c3a7oB_ not modelled 5.3 29 PDB header:protein transport Chain: B: PDB Molecule:autophagy protein 16; PDBTitle: the crystal structure of the coiled-coil domain of2 saccharomyces cerevisiae atg16

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0