Phyre Home Retrieve Phyre Job Id
Emaill.a.kelley@imperial.ac.uk
DescriptionP0AFS5
DateThu Jan 5 11:27:09 GMT 2012
Unique Job ID615be1169d6a7953

Summary 

Top model
Image coloured by rainbow N → C terminus
Model (left) based on template c3rlbA_
Top template information
PDB header:thiamine-binding protein
Chain: A: PDB Molecule:thit;
PDBTitle: crystal structure at 2.0 a of the s-component for thiamin from an ecf-2 type abc transporter
Confidence and coverage
Confidence: 12.8% Coverage: 32%
110 residues ( 32% of your sequence) have been modelled with 12.8% confidence by the single highest scoring template.
You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library.
Please note: You must be registered and logged in to use Phyrealarm.
3D viewing
Interactive 3D view in Jmol

Sequence analysis 

Secondary structure and disorder prediction 

   1........10.........20.........30.........40.........50.........60
Sequence  MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIAVILNPLVQHMVRWRVP
Secondary structure 












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   .........70.........80.........90.........100.........110.........120
Sequence  RVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIMTPLQALEPLLQRVGIDVS
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??
???
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   .........130.........140.........150.........160.........170.........180
Sequence  VDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLFMLLEVPQLPGKFQQMMARPV
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   .........190.........200.........210.........220.........230.........240
Sequence  EGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAALDVRFAFVWGLLAFALNYIPNIG
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   .........250.........260.........270.........280.........290.........300
Sequence  SVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGNILEPRIMGRGLGLSTLVVFLSLI
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   .........310.........320.........330.........340....
Sequence  FWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSIAVLLSDLNKE
Secondary structure 





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??????
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Confidence Key
High(9)                    Low (0)
?Disordered
Alpha helix
Beta strand

Domain analysis 

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

RankAligned region

PDB 3rlb chain A

3D model

Region: 225 - 344
Aligned: 110
Modelled: 120
Confidence: 12.8%
Identity: 25%
PDB header:thiamine-binding protein
Chain: A: PDB Molecule:thit;
PDBTitle: crystal structure at 2.0 a of the s-component for thiamin from an ecf-2 type abc transporter

Phyre2

PDB 1ppj chain C domain 2

3D model

Region: 182 - 247
Aligned: 66
Modelled: 66
Confidence: 9.7%
Identity: 11%
Fold: Heme-binding four-helical bundle
Superfamily: Transmembrane di-heme cytochromes
Family: Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)

Phyre2

PDB 3rce chain A

3D model

Region: 13 - 107
Aligned: 95
Modelled: 95
Confidence: 8.3%
Identity: 13%
PDB header:transferase/peptide
Chain: A: PDB Molecule:oligosaccharide transferase to n-glycosylate proteins;
PDBTitle: bacterial oligosaccharyltransferase pglb

Phyre2

PDB 2oar chain A

3D model

Region: 142 - 195
Aligned: 54
Modelled: 54
Confidence: 7.3%
Identity: 15%
PDB header:membrane protein
Chain: A: PDB Molecule:large-conductance mechanosensitive channel;
PDBTitle: mechanosensitive channel of large conductance (mscl)

Phyre2

PDB 1ehk chain B domain 2

3D model

Region: 28 - 47
Aligned: 20
Modelled: 20
Confidence: 6.3%
Identity: 25%
Fold: Transmembrane helix hairpin
Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region
Family: Cytochrome c oxidase subunit II-like, transmembrane region

Phyre2

PDB 3cwb chain C

3D model

Region: 182 - 247
Aligned: 66
Modelled: 66
Confidence: 5.9%
Identity: 9%
PDB header:oxidoreductase
Chain: C: PDB Molecule:cytochrome b;
PDBTitle: chicken cytochrome bc1 complex inhibited by an iodinated analogue of2 the polyketide crocacin-d

Phyre2

PDB 2lcy chain A

3D model

Region: 236 - 241
Aligned: 6
Modelled: 6
Confidence: 5.8%
Identity: 50%
PDB header:viral protein
Chain: A: PDB Molecule:virion spike glycoprotein;
PDBTitle: nmr structure of the complete internal fusion loop from ebolavirus gp22 at ph 5.5

Phyre2
1

c3rlbA_
2

d1ppjc2
3

c3rceA_
4

c2oarA_
5

d1ehkb2
6

c3cwbC_
7

c2lcyA_



Detailed template information 

#
Template Alignment Coverage3D Model Confidence
% i.d. Template Information
1c3rlbA_



12.8 25 PDB header:thiamine-binding protein
Chain: A: PDB Molecule:thit;
PDBTitle: crystal structure at 2.0 a of the s-component for thiamin from an ecf-2 type abc transporter
2d1ppjc2



9.7 11 Fold:Heme-binding four-helical bundle
Superfamily:Transmembrane di-heme cytochromes
Family:Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
3c3rceA_



8.3 13 PDB header:transferase/peptide
Chain: A: PDB Molecule:oligosaccharide transferase to n-glycosylate proteins;
PDBTitle: bacterial oligosaccharyltransferase pglb
4c2oarA_



7.3 15 PDB header:membrane protein
Chain: A: PDB Molecule:large-conductance mechanosensitive channel;
PDBTitle: mechanosensitive channel of large conductance (mscl)
5d1ehkb2



6.3 25 Fold:Transmembrane helix hairpin
Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region
Family:Cytochrome c oxidase subunit II-like, transmembrane region
6c3cwbC_



5.9 9 PDB header:oxidoreductase
Chain: C: PDB Molecule:cytochrome b;
PDBTitle: chicken cytochrome bc1 complex inhibited by an iodinated analogue of2 the polyketide crocacin-d
7c2lcyA_



5.8 50 PDB header:viral protein
Chain: A: PDB Molecule:virion spike glycoprotein;
PDBTitle: nmr structure of the complete internal fusion loop from ebolavirus gp22 at ph 5.5

Binding site prediction 

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction 

Transmembrane helices have been predicted in your sequence to adopt the topology shown below



Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
 
© Structural Bioinformatics Group
Imperial College London
Lawrence Kelley, Benjamin Jefferys
Disclaimer
Terms and Conditions
Component software
Template detection: HHpred 1.51
Secondary structure prediction: Psi-pred 2.5
Disorder prediction: Disopred 2.4
Transmembrane prediction: Memsat_SVM
Multi-template modelling and ab initio: Poing 1.0