Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	Y	V	I	Y	A	Q	D	K	A	D	S	L	E	K	R	L	S	V	R	P	A	H	L	A	R	L	Q	L	L	H	D	E	G	R	L	L	T	A	G	P	M	P	A	V	D	S	N	D	P	G	A	A	G	F	T	G	S	T
Secondary structure
SS confidence
Disorder		?																																															?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.
Sequence		V	I	A	E	F	E	S	L	E	A	A	Q	A	W	A	D	A	D	P	Y	V	A	A	G	V	Y	E	H	V	S	V	K	P	F	K	K	V	F
Secondary structure
SS confidence
Disorder																																						?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1mwq chain A Region: 2 - 98 Aligned: 97 Modelled: 97 Confidence: 100.0% Identity: 67% Fold: Ferredoxin-like Superfamily: Dimeric alpha+beta barrel Family: YciI-like Phyre2 PDB 1s7i chain A Region: 1 - 93 Aligned: 90 Modelled: 89 Confidence: 99.0% Identity: 18% Fold: Ferredoxin-like Superfamily: Dimeric alpha+beta barrel Family: DGPF domain (Pfam 04946) Phyre2 PDB 1mli chain A Region: 1 - 93 Aligned: 80 Modelled: 83 Confidence: 95.8% Identity: 19% Fold: Ferredoxin-like Superfamily: Dimeric alpha+beta barrel Family: Muconalactone isomerase, MLI Phyre2 PDB 3lo3 chain E Region: 1 - 82 Aligned: 75 Modelled: 82 Confidence: 58.9% Identity: 23% PDB header:structure genomics, unknown function Chain: E: PDB Molecule:uncharacterized conserved protein; PDBTitle: the crystal structure of a conserved functionally unknown2 protein from colwellia psychrerythraea 34h. Phyre2 PDB 2fiu chain A domain 1 Region: 4 - 82 Aligned: 72 Modelled: 74 Confidence: 49.8% Identity: 19% Fold: Ferredoxin-like Superfamily: Dimeric alpha+beta barrel Family: Atu0297-like Phyre2 PDB 3dca chain C Region: 1 - 83 Aligned: 79 Modelled: 82 Confidence: 23.8% Identity: 16% PDB header:structural genomics, unknown function Chain: C: PDB Molecule:rpa0582; PDBTitle: crystal structure of the rpa0582- protein of unknown2 function from rhodopseudomonas palustris- a structural3 genomics target Phyre2 PDB 2f06 chain A domain 1 Region: 3 - 80 Aligned: 59 Modelled: 58 Confidence: 23.2% Identity: 10% Fold: Ferredoxin-like Superfamily: ACT-like Family: BT0572-like Phyre2 PDB 2dtc chain B Region: 58 - 76 Aligned: 19 Modelled: 19 Confidence: 11.4% Identity: 21% PDB header:protein binding Chain: B: PDB Molecule:ral guanine nucleotide exchange factor ralgps1a; PDBTitle: crystal structure of ms0666 Phyre2 PDB 2aya chain A Region: 68 - 82 Aligned: 15 Modelled: 15 Confidence: 10.0% Identity: 27% PDB header:transferase Chain: A: PDB Molecule:dna polymerase iii subunit tau; PDBTitle: solution structure of the c-terminal 14 kda domain of the2 tau subunit from escherichia coli dna polymerase iii Phyre2 PDB 1btn chain A Region: 57 - 77 Aligned: 21 Modelled: 21 Confidence: 9.6% Identity: 19% Fold: PH domain-like barrel Superfamily: PH domain-like Family: Pleckstrin-homology domain (PH domain) Phyre2 PDB 2p0f chain A Region: 58 - 77 Aligned: 20 Modelled: 20 Confidence: 8.3% Identity: 20% PDB header:ligand binding protein Chain: A: PDB Molecule:rho gtpase-activating protein 9; PDBTitle: arhgap9 ph domain in complex with ins(1,3,5)p3 Phyre2 PDB 2gp4 chain A domain 2 Region: 15 - 43 Aligned: 29 Modelled: 29 Confidence: 8.3% Identity: 21% Fold: IlvD/EDD N-terminal domain-like Superfamily: IlvD/EDD N-terminal domain-like Family: lvD/EDD N-terminal domain-like Phyre2 PDB 1o7d chain A Region: 18 - 48 Aligned: 31 Modelled: 31 Confidence: 8.1% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:lysosomal alpha-mannosidase; PDBTitle: the structure of the bovine lysosomal a-mannosidase2 suggests a novel mechanism for low ph activation Phyre2 PDB 2wyh chain A Region: 14 - 48 Aligned: 35 Modelled: 35 Confidence: 7.9% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:alpha-mannosidase; PDBTitle: structure of the streptococcus pyogenes family gh38 alpha-2 mannosidase Phyre2 PDB 2dhx chain A Region: 26 - 93 Aligned: 58 Modelled: 68 Confidence: 7.0% Identity: 22% PDB header:rna binding protein Chain: A: PDB Molecule:poly (adp-ribose) polymerase family, member 10 PDBTitle: solution structure of the rrm domain in the human poly (adp-2 ribose) polymerase family, member 10 variant Phyre2 PDB 1dro chain A Region: 58 - 77 Aligned: 20 Modelled: 20 Confidence: 6.2% Identity: 10% Fold: PH domain-like barrel Superfamily: PH domain-like Family: Pleckstrin-homology domain (PH domain) Phyre2 PDB 2e7x chain A Region: 21 - 83 Aligned: 63 Modelled: 63 Confidence: 6.1% Identity: 11% PDB header:transcription regulator Chain: A: PDB Molecule:150aa long hypothetical transcriptional regulator; PDBTitle: structure of the lrp/asnc like transcriptional regulator from2 sulfolobus tokodaii 7 complexed with its cognate ligand Phyre2 PDB 1wjm chain A Region: 58 - 77 Aligned: 20 Modelled: 20 Confidence: 6.1% Identity: 10% Fold: PH domain-like barrel Superfamily: PH domain-like Family: Pleckstrin-homology domain (PH domain) Phyre2 PDB 2hfu chain B Region: 22 - 93 Aligned: 59 Modelled: 72 Confidence: 6.1% Identity: 17% PDB header:transferase Chain: B: PDB Molecule:mevalonate kinase, putative; PDBTitle: crystal structure of l. major mevalonate kinase in complex2 with r-mevalonate Phyre2 PDB 2l4c chain A Region: 62 - 88 Aligned: 27 Modelled: 27 Confidence: 6.0% Identity: 15% PDB header:peptide binding protein Chain: A: PDB Molecule:endoplasmic reticulum resident protein 27; PDBTitle: solution structure of the b domain of human erp27 Phyre2 1 d1mwqa_ 2 d1s7ia_ 3 d1mlia_ 4 c3lo3E_ 5 d2fiua1 6 c3dcaC_ 7 d2f06a1 8 c2dtcB_ 9 c2ayaA_ 10 d1btna_ 11 c2p0fA_ 12 d2gp4a2 13 c1o7dA_ 14 c2wyhA_ 15 c2dhxA_ 16 d1droa_ 17 c2e7xA_ 18 d1wjma_ 19 c2hfuB_ 20 c2l4cA_ 21 22 23 24 25 26

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1mwqa_ 100.0 67 Fold:Ferredoxin-like Superfamily:Dimeric alpha+beta barrel Family:YciI-like 2 d1s7ia_ 99.0 18 Fold:Ferredoxin-like Superfamily:Dimeric alpha+beta barrel Family:DGPF domain (Pfam 04946) 3 d1mlia_ 95.8 19 Fold:Ferredoxin-like Superfamily:Dimeric alpha+beta barrel Family:Muconalactone isomerase, MLI 4 c3lo3E_ 58.9 23 PDB header:structure genomics, unknown function Chain: E: PDB Molecule:uncharacterized conserved protein; PDBTitle: the crystal structure of a conserved functionally unknown2 protein from colwellia psychrerythraea 34h. 5 d2fiua1 49.8 19 Fold:Ferredoxin-like Superfamily:Dimeric alpha+beta barrel Family:Atu0297-like 6 c3dcaC_ 23.8 16 PDB header:structural genomics, unknown function Chain: C: PDB Molecule:rpa0582; PDBTitle: crystal structure of the rpa0582- protein of unknown2 function from rhodopseudomonas palustris- a structural3 genomics target 7 d2f06a1 23.2 10 Fold:Ferredoxin-like Superfamily:ACT-like Family:BT0572-like 8 c2dtcB_ 11.4 21 PDB header:protein binding Chain: B: PDB Molecule:ral guanine nucleotide exchange factor ralgps1a; PDBTitle: crystal structure of ms0666 9 c2ayaA_ 10.0 27 PDB header:transferase Chain: A: PDB Molecule:dna polymerase iii subunit tau; PDBTitle: solution structure of the c-terminal 14 kda domain of the2 tau subunit from escherichia coli dna polymerase iii 10 d1btna_ 9.6 19 Fold:PH domain-like barrel Superfamily:PH domain-like Family:Pleckstrin-homology domain (PH domain) 11 c2p0fA_ 8.3 20 PDB header:ligand binding protein Chain: A: PDB Molecule:rho gtpase-activating protein 9; PDBTitle: arhgap9 ph domain in complex with ins(1,3,5)p3 12 d2gp4a2 8.3 21 Fold:IlvD/EDD N-terminal domain-like Superfamily:IlvD/EDD N-terminal domain-like Family:lvD/EDD N-terminal domain-like 13 c1o7dA_ 8.1 23 PDB header:hydrolase Chain: A: PDB Molecule:lysosomal alpha-mannosidase; PDBTitle: the structure of the bovine lysosomal a-mannosidase2 suggests a novel mechanism for low ph activation 14 c2wyhA_ 7.9 23 PDB header:hydrolase Chain: A: PDB Molecule:alpha-mannosidase; PDBTitle: structure of the streptococcus pyogenes family gh38 alpha-2 mannosidase 15 c2dhxA_ 7.0 22 PDB header:rna binding protein Chain: A: PDB Molecule:poly (adp-ribose) polymerase family, member 10 PDBTitle: solution structure of the rrm domain in the human poly (adp-2 ribose) polymerase family, member 10 variant 16 d1droa_ 6.2 10 Fold:PH domain-like barrel Superfamily:PH domain-like Family:Pleckstrin-homology domain (PH domain) 17 c2e7xA_ 6.1 11 PDB header:transcription regulator Chain: A: PDB Molecule:150aa long hypothetical transcriptional regulator; PDBTitle: structure of the lrp/asnc like transcriptional regulator from2 sulfolobus tokodaii 7 complexed with its cognate ligand 18 d1wjma_ 6.1 10 Fold:PH domain-like barrel Superfamily:PH domain-like Family:Pleckstrin-homology domain (PH domain) 19 c2hfuB_ 6.1 17 PDB header:transferase Chain: B: PDB Molecule:mevalonate kinase, putative; PDBTitle: crystal structure of l. major mevalonate kinase in complex2 with r-mevalonate 20 c2l4cA_ 6.0 15 PDB header:peptide binding protein Chain: A: PDB Molecule:endoplasmic reticulum resident protein 27; PDBTitle: solution structure of the b domain of human erp27 21 c2z0rA_ not modelled 5.6 38 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:putative uncharacterized protein ttha0547; PDBTitle: crystal structure of hypothetical protein ttha0547 22 c3bsuF_ not modelled 5.4 23 PDB header:hydrolase/rna/dna Chain: F: PDB Molecule:ribonuclease h1; PDBTitle: hybrid-binding domain of human rnase h1 in complex with 12-2 mer rna/dna 23 d1ueka2 not modelled 5.3 25 Fold:Ferredoxin-like Superfamily:GHMP Kinase, C-terminal domain Family:4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol kinase IspE 24 d2coca1 not modelled 5.3 20 Fold:PH domain-like barrel Superfamily:PH domain-like Family:Pleckstrin-homology domain (PH domain) 25 c2hjdA_ not modelled 5.2 17 PDB header:signaling protein Chain: A: PDB Molecule:quorum-sensing antiactivator; PDBTitle: crystal structure of a second quorum sensing antiactivator tram2 from2 a. tumefaciens strain a6 26 d1d6za3 not modelled 5.0 18 Fold:Cystatin-like Superfamily:Amine oxidase N-terminal region Family:Amine oxidase N-terminal region

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0