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Job DescriptionP0AG78
Confidence99.82%DateWed Jan 25 15:20:33 GMT 2012
Rank57Aligned Residues305
% Identity23%Templatec3osqA_
PDB info PDB header:fluorescent protein, transport proteinChain: A: PDB Molecule:maltose-binding periplasmic protein, green fluorescent PDBTitle: maltose-bound maltose sensor engineered by insertion of circularly2 permuted green fluorescent protein into e. coli maltose binding3 protein at position 175
Resolution1.90 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   20....... ..30.........40.........50.........60.........70.........80.........90........
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Query Sequence  KDIQLLNV. SYDPTRELYEQYNKAFSAHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRID
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Template Sequence  EEGKLVIWINGDKGYNGLAEVGKKFEK. . . . DTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFW. AHDRFGGYAQSGLLA
Template Known Secondary structure 
TTS

TTS
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TTTT
S
S.GGGTT
B
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   3......10.........20......... 30.........40.........50.........60.. .......70.......
 
   99100...... ...110.........120.... .....130.... .....140.........150...
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Query Sequence  KEWIKRLP. . . . . . . . . . . . . . . . . . DNSAPYTSTIVFLVRKGN. PKQIHDWNDL. . . . . . IKPGVSVITPNPKSSGGAR
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Template Sequence  EITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFT
Template Known Secondary structure 




TTB
TTT
S

TTT
SS

SBSTTTTT




SS
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   78.80.........90.........100.........110.........120.........130.........140.........150.......
 
   154.....160.
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Query Sequence  WNYLAAWG. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Conservation         
                                                                        
Template Sequence  WPLIAADGGYAFKYENHLSHNVYIMADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSTQSKLS
Template Known Secondary structure  TT

S


GGGTBTTS
SSSS





S
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   158.160.........170.........180.........190.........200.........210.........220.........230.......
 
  
Predicted Secondary structure  ................................................................................
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation                                                                                  
Template Sequence  KDPNEKRDHMVLLEFVTAAGITLGMDELYKGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
Template Known Secondary structure 

TT

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TT


TGGGGGSSTTTTTTT
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   238.240.........250.........260.........270.........280.........290.........300.........310.......
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation                                                                                  
Template Sequence  TGKLPVPWPTLVTTLVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKE
Template Known Secondary structure  TSS
SS
GGGGTTT

GGG
B

GGGGGG
TTTT
TTS
TTS

T
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   324.....330.........340.........350.........360.........370.........380.........390.........400...
 
   162.......170.........180.........190.........200.........210.........220.
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Query Sequence  . . . . . . . . . . . . . . . . . . . . YALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLAANE
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Template Sequence  DGNILGHKLEYNFNGGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTS
Template Known Secondary structure  TSTTTTT
B
SSSTTTTSSSTTSS
TT

TTS
GGG
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   407..410.........420.........430.........440.........450.........460.........470.........480......
 
   222.......230. ........240.........250.........260.........270.... .....280.......
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Query Sequence  LGKDKFEIVT. PSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIA. AKNYYRPRDAEVA. . . . . . . . . . . .
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Template Sequence  KVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIA
Template Known Secondary structure  T




BBTTB



BTT
S
TTSSS


SBTTS
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   487..490.........500.........510.........520.........530.........540.........550.........560......
 
   288.290.........300.........310.........320.........
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Query Sequence  . . . KKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR
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Template Sequence  ATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEDLKD
Template Known Secondary structure  S



TTTTSS
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   567..570.........580.........590.........600.........610.
 
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No model constructed - rank, confidence too low




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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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