Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	R	Y	T	P	D	F	P	E	M	M	R	L	C	E	M	N	F	S	Q	L	R	R	L	L	P	R	N	D	A	P	G	E	T	V	S	Y	Q	V	A	N	A	Q	Y	R	L	T	I	V	E	S	T	R	Y	T	T	L	V	T
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																									?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	E	Q	T	A	P	A	I	S	Y	W	S	L	P	S	M	T	V	R	L	Y	H	D	A	M	V	A	E	V	C	S	S	Q	Q	I	F	R	F	K	A	R	Y	D	Y	P	N	K	K	L	H	Q	R	D	E	K	H	Q	I	N	Q
Secondary structure
SS confidence
Disorder							?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140
Sequence		F	L	A	D	W	L	R	Y	C	L	A	H	G	A	M	A	I	P	V	Y
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1m47 chain A Region: 113 - 129 Aligned: 17 Modelled: 17 Confidence: 42.7% Identity: 41% Fold: 4-helical cytokines Superfamily: 4-helical cytokines Family: Short-chain cytokines Phyre2 PDB 2keb chain A Region: 121 - 139 Aligned: 19 Modelled: 19 Confidence: 27.5% Identity: 5% PDB header:dna binding protein Chain: A: PDB Molecule:dna polymerase subunit alpha b; PDBTitle: nmr solution structure of the n-terminal domain of the dna polymerase2 alpha p68 subunit Phyre2 PDB 3ige chain A Region: 39 - 60 Aligned: 22 Modelled: 22 Confidence: 23.5% Identity: 41% PDB header:viral protein Chain: A: PDB Molecule:soc small outer capsid protein; PDBTitle: small outer capsid protein (soc) from bacteriophage rb69 Phyre2 PDB 3b7s chain A domain 1 Region: 103 - 133 Aligned: 31 Modelled: 31 Confidence: 20.3% Identity: 23% Fold: alpha-alpha superhelix Superfamily: ARM repeat Family: Leukotriene A4 hydrolase C-terminal domain Phyre2 PDB 1f44 chain A domain 1 Region: 122 - 139 Aligned: 18 Modelled: 18 Confidence: 19.3% Identity: 17% Fold: SAM domain-like Superfamily: lambda integrase-like, N-terminal domain Family: lambda integrase-like, N-terminal domain Phyre2 PDB 3iab chain B Region: 55 - 81 Aligned: 27 Modelled: 27 Confidence: 15.5% Identity: 15% PDB header:hydrolase/rna Chain: B: PDB Molecule:ribonucleases p/mrp protein subunit pop7; PDBTitle: crystal structure of rnase p /rnase mrp proteins pop6, pop72 in a complex with the p3 domain of rnase mrp rna Phyre2 PDB 3h6p chain D Region: 109 - 127 Aligned: 19 Modelled: 19 Confidence: 15.4% Identity: 32% PDB header:structural genomics, unknown function Chain: D: PDB Molecule:esat-6-like protein esxr; PDBTitle: crystal structure of rv3019c-rv3020c from mycobacterium tuberculosis Phyre2 PDB 1iv8 chain A domain 1 Region: 41 - 80 Aligned: 40 Modelled: 40 Confidence: 14.6% Identity: 28% Fold: Glycosyl hydrolase domain Superfamily: Glycosyl hydrolase domain Family: alpha-Amylases, C-terminal beta-sheet domain Phyre2 PDB 3g36 chain D Region: 120 - 126 Aligned: 7 Modelled: 7 Confidence: 14.6% Identity: 57% PDB header:nuclear protein Chain: D: PDB Molecule:protein dpy-30 homolog; PDBTitle: crystal structure of the human dpy-30-like c-terminal domain Phyre2 PDB 2dsy chain A domain 1 Region: 113 - 140 Aligned: 27 Modelled: 28 Confidence: 13.6% Identity: 26% Fold: TTHA1013/TTHA0281-like Superfamily: TTHA1013/TTHA0281-like Family: TTHA0281-like Phyre2 PDB 3iu0 chain A Region: 87 - 131 Aligned: 39 Modelled: 45 Confidence: 12.4% Identity: 26% PDB header:transferase Chain: A: PDB Molecule:protein-glutamine gamma-glutamyltransferase; PDBTitle: structural basis for zymogen activation and substrate binding of2 transglutaminase from streptomyces mobaraense Phyre2 PDB 1xsq chain A Region: 36 - 61 Aligned: 26 Modelled: 26 Confidence: 10.4% Identity: 23% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Ureidoglycolate hydrolase AllA Phyre2 PDB 1ufo chain A Region: 104 - 127 Aligned: 24 Modelled: 24 Confidence: 10.1% Identity: 17% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Hypothetical protein TT1662 Phyre2 PDB 1mky chain A domain 3 Region: 117 - 126 Aligned: 10 Modelled: 10 Confidence: 9.1% Identity: 40% Fold: Alpha-lytic protease prodomain-like Superfamily: Probable GTPase Der, C-terminal domain Family: Probable GTPase Der, C-terminal domain Phyre2 PDB 1t15 chain A domain 1 Region: 124 - 134 Aligned: 11 Modelled: 11 Confidence: 8.6% Identity: 9% Fold: BRCT domain Superfamily: BRCT domain Family: BRCT domain Phyre2 PDB 1xhm chain B domain 1 Region: 123 - 136 Aligned: 14 Modelled: 14 Confidence: 8.4% Identity: 43% Fold: Non-globular all-alpha subunits of globular proteins Superfamily: Transducin (heterotrimeric G protein), gamma chain Family: Transducin (heterotrimeric G protein), gamma chain Phyre2 PDB 1xhm chain B Region: 123 - 136 Aligned: 14 Modelled: 14 Confidence: 8.2% Identity: 43% PDB header:signaling protein Chain: B: PDB Molecule:guanine nucleotide-binding protein g(i)/g(s) PDBTitle: the crystal structure of a biologically active peptide2 (sigk) bound to a g protein beta:gamma heterodimer Phyre2 PDB 1iu4 chain A Region: 87 - 131 Aligned: 39 Modelled: 45 Confidence: 7.7% Identity: 26% Fold: Cysteine proteinases Superfamily: Cysteine proteinases Family: Microbial transglutaminase Phyre2 PDB 2jya chain A Region: 76 - 87 Aligned: 12 Modelled: 12 Confidence: 6.5% Identity: 33% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein atu1810; PDBTitle: nmr solution structure of protein atu1810 from agrobacterium2 tumefaciens. northeast structural genomics consortium target atr23,3 ontario centre for structural proteomics target atc1776 Phyre2 PDB 1l0b chain A domain 1 Region: 124 - 134 Aligned: 11 Modelled: 11 Confidence: 6.4% Identity: 9% Fold: BRCT domain Superfamily: BRCT domain Family: BRCT domain Phyre2 1 d1m47a_ 2 c2kebA_ 3 c3igeA_ 4 d3b7sa1 5 d1f44a1 6 c3iabB_ 7 c3h6pD_ 8 d1iv8a1 9 c3g36D_ 10 d2dsya1 11 c3iu0A_ 12 d1xsqa_ 13 d1ufoa_ 14 d1mkya3 15 d1t15a1 16 d1xhmb1 17 c1xhmB_ 18 d1iu4a_ 19 c2jyaA_ 20 d1l0ba1 21 22 23

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1m47a_ 42.7 41 Fold:4-helical cytokines Superfamily:4-helical cytokines Family:Short-chain cytokines 2 c2kebA_ 27.5 5 PDB header:dna binding protein Chain: A: PDB Molecule:dna polymerase subunit alpha b; PDBTitle: nmr solution structure of the n-terminal domain of the dna polymerase2 alpha p68 subunit 3 c3igeA_ 23.5 41 PDB header:viral protein Chain: A: PDB Molecule:soc small outer capsid protein; PDBTitle: small outer capsid protein (soc) from bacteriophage rb69 4 d3b7sa1 20.3 23 Fold:alpha-alpha superhelix Superfamily:ARM repeat Family:Leukotriene A4 hydrolase C-terminal domain 5 d1f44a1 19.3 17 Fold:SAM domain-like Superfamily:lambda integrase-like, N-terminal domain Family:lambda integrase-like, N-terminal domain 6 c3iabB_ 15.5 15 PDB header:hydrolase/rna Chain: B: PDB Molecule:ribonucleases p/mrp protein subunit pop7; PDBTitle: crystal structure of rnase p /rnase mrp proteins pop6, pop72 in a complex with the p3 domain of rnase mrp rna 7 c3h6pD_ 15.4 32 PDB header:structural genomics, unknown function Chain: D: PDB Molecule:esat-6-like protein esxr; PDBTitle: crystal structure of rv3019c-rv3020c from mycobacterium tuberculosis 8 d1iv8a1 14.6 28 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 9 c3g36D_ 14.6 57 PDB header:nuclear protein Chain: D: PDB Molecule:protein dpy-30 homolog; PDBTitle: crystal structure of the human dpy-30-like c-terminal domain 10 d2dsya1 13.6 26 Fold:TTHA1013/TTHA0281-like Superfamily:TTHA1013/TTHA0281-like Family:TTHA0281-like 11 c3iu0A_ 12.4 26 PDB header:transferase Chain: A: PDB Molecule:protein-glutamine gamma-glutamyltransferase; PDBTitle: structural basis for zymogen activation and substrate binding of2 transglutaminase from streptomyces mobaraense 12 d1xsqa_ 10.4 23 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:Ureidoglycolate hydrolase AllA 13 d1ufoa_ 10.1 17 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:Hypothetical protein TT1662 14 d1mkya3 9.1 40 Fold:Alpha-lytic protease prodomain-like Superfamily:Probable GTPase Der, C-terminal domain Family:Probable GTPase Der, C-terminal domain 15 d1t15a1 8.6 9 Fold:BRCT domain Superfamily:BRCT domain Family:BRCT domain 16 d1xhmb1 8.4 43 Fold:Non-globular all-alpha subunits of globular proteins Superfamily:Transducin (heterotrimeric G protein), gamma chain Family:Transducin (heterotrimeric G protein), gamma chain 17 c1xhmB_ 8.2 43 PDB header:signaling protein Chain: B: PDB Molecule:guanine nucleotide-binding protein g(i)/g(s) PDBTitle: the crystal structure of a biologically active peptide2 (sigk) bound to a g protein beta:gamma heterodimer 18 d1iu4a_ 7.7 26 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:Microbial transglutaminase 19 c2jyaA_ 6.5 33 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein atu1810; PDBTitle: nmr solution structure of protein atu1810 from agrobacterium2 tumefaciens. northeast structural genomics consortium target atr23,3 ontario centre for structural proteomics target atc1776 20 d1l0ba1 6.4 9 Fold:BRCT domain Superfamily:BRCT domain Family:BRCT domain 21 c3ktfB_ not modelled 6.3 36 PDB header:cell cycle Chain: B: PDB Molecule:microcephalin; PDBTitle: structure of the n-terminal brct domain of human2 microcephalin (mcph1). 22 c3ebrA_ not modelled 6.2 43 PDB header:unknown function Chain: A: PDB Molecule:uncharacterized rmlc-like cupin; PDBTitle: crystal structure of an rmlc-like cupin protein (reut_a0381) from2 ralstonia eutropha jmp134 at 2.60 a resolution 23 c2cokA_ not modelled 5.3 19 PDB header:transferase Chain: A: PDB Molecule:poly [adp-ribose] polymerase-1; PDBTitle: solution structure of brct domain of poly(adp-ribose)2 polymerase-1

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0