Job Description P09099 Confidence 100.00% Date Wed Jan 25 15:20:14 GMT 2012 Rank 15 Aligned Residues 460 % Identity 23% Template c3ezwD_ PDB info PDB header:transferase Chain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices Resolution 2.00 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.			.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.
Predicted Secondary structure																																																																.	.
Query SS confidence																																																																.	.
Query Sequence		Y	I	G	I	D	L	G	T	S	G	V	K	V	I	L	L	N	E	Q	G	E	V	V	A	A	Q	T	E	K	L	T	V	S	R	P	H	P	L	W	S	E	Q	D	P	E	Q	W	W	Q	A	T	D	R	A	M	K	A	L	G	D	Q	H	.	.	S	L	Q	D	V	K	A	L	G	I	A	G	Q	M	H	G
Query Conservation																																																																.	.
Alig confidence																																																																.	.
Template Conservation
Template Sequence		I	V	A	L	D	Q	G	T	T	S	S	R	A	V	V	M	D	H	D	A	N	I	I	S	V	S	Q	R	E	F	E	Q	I	Y	P	K	P	G	W	V	E	H	D	P	M	E	I	W	A	T	Q	S	S	T	L	V	E	V	L	A	K	A	D	I	S	S	D	Q	I	A	A	I	G	I	T	N	Q	R	E	T
Template Known Secondary structure									S	S									T	T														B			S	T	T																								T	T			G	G	G										S	S
Template Predicted Secondary structure
Template SS confidence
		6	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.
		80	.	.	.	.	.		.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.			.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.					.	150	.	.
Predicted Secondary structure								.																													.	.																																				.	.	.	.
Query SS confidence								.																													.	.																																				.	.	.	.
Query Sequence		A	T	L	L	D	A	.	Q	Q	R	V	L	R	P	A	I	L	W	N	D	G	R	C	A	Q	E	C	T	L	L	E	A	R	V	P	.	.	Q	S	R	V	I	T	G	N	L	M	M	P	G	F	T	A	P	K	L	L	W	V	Q	R	H	E	P	E	I	F	R	Q	I	D	K	.	.	.	.	V	L	L	P
Query Conservation								.																													.	.																																				.	.	.	.
Alig confidence								.																													.	.																																				.	.	.	.
Template Conservation
Template Sequence		T	I	V	W	E	K	E	T	G	K	P	I	Y	N	A	I	V	W	Q	C	R	R	T	A	E	I	C	E	H	L	K	R	D	G	L	E	D	Y	I	R	S	N	T	G	L	V	I	D	P	Y	F	S	G	T	K	V	K	W	I	L	D	H	V	E	G	S	R	E	R	A	R	R	G	E	L	L	F	G	T	V
Template Known Secondary structure		B					T	T	T			B	S	S					T	T			T	T										T	T										S			S	S	T	T	S												S	T	T							T	T	S
Template Predicted Secondary structure
Template SS confidence
		86	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.
		153	.	.	.	.	.	.	160	.	.			.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230
Predicted Secondary structure												.	.
Query SS confidence												.	.
Query Sequence		K	D	Y	L	R	L	R	M	T	G	.	.	E	F	A	S	D	M	S	D	A	A	G	T	M	W	L	D	V	A	K	R	D	W	S	D	V	M	L	Q	A	C	D	L	S	R	D	Q	M	P	A	L	Y	E	G	S	E	I	T	G	A	L	L	P	E	V	A	K	A	W	G	M	A	T	V	P	V	V	A	G
Query Conservation												.	.
Alig confidence												.	.																																																			.	.	.	.	.	.	.	.	.	.
Template Conservation																																																																.	.	.	.	.	.	.	.	.	.
Template Sequence		D	T	W	L	I	W	K	M	T	Q	G	R	V	H	V	T	D	Y	T	N	A	S	R	T	M	L	F	N	I	H	T	L	D	W	D	D	K	M	L	E	V	L	D	I	P	R	E	M	L	P	E	V	R	R	S	S	E	V	Y	G	Q	T	.	.	.	.	.	.	.	.	.	.	N	R	I	P	I	S	G	I
Template Known Secondary structure										T	T	T	S										G	G	G	S	S			T	T	T	T										T	T			G	G	G							S								.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																																																																.	.	.	.	.	.	.	.	.	.
Template SS confidence
		166	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.											.	.	230	.	.	.	.	.
		231	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.				.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.
Predicted Secondary structure																																																							.	.	.
Query SS confidence																																																							.	.	.
Query Sequence		G	G	D	N	A	A	G	A	V	G	V	G	M	V	D	A	N	Q	A	M	L	S	L	G	T	S	G	V	Y	F	A	V	S	E	G	F	L	S	K	P	E	S	A	V	H	S	F	C	H	A	L	P	Q	.	.	.	R	W	H	L	M	S	V	M	L	S	A	A	S	C	L	D	W	A	A	K	L	T	G	L
Query Conservation																																																							.	.	.
Alig confidence																																																							.	.	.
Template Conservation
Template Sequence		A	G	D	Q	Q	A	A	L	F	G	Q	L	C	V	K	E	G	M	A	K	N	T	Y	G	T	G	C	F	M	L	M	N	T	G	E	K	A	V	K	S	E	N	G	L	L	T	T	I	A	C	G	P	T	G	E	V	N	Y	A	L	E	G	A	V	F	M	A	G	A	S	I	Q	W	L	R	D	E	M	K	L
Template Known Secondary structure												T	T			S	T	T							S	S	S								T	T	S					S	S	S									T	T	S											S		S										T	S
Template Predicted Secondary structure
Template SS confidence
		243	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.
		308	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.		.	.	.	.
Predicted Secondary structure																																																																													.
Query SS confidence																																																																													.
Query Sequence		S	N	V	P	A	L	I	A	A	A	Q	Q	A	D	E	S	A	E	P	V	W	F	L	P	Y	L	S	G	E	R	T	P	H	N	N	P	Q	A	K	G	V	F	F	G	L	T	H	Q	H	G	P	N	E	L	A	R	A	V	L	E	G	V	G	Y	A	L	A	D	G	M	D	V	V	H	A	.	C	G	I	K
Query Conservation																																																																													.
Alig confidence				.	.	.	.																																																																						.
Template Conservation				.	.	.	.
Template Sequence		I	D	.	.	.	.	S	E	Y	F	A	T	K	V	Q	N	T	N	G	V	Y	V	V	P	A	F	T	G	L	G	A	P	Y	W	D	P	Y	A	R	G	A	I	F	G	L	T	R	G	V	N	A	N	H	I	I	R	A	T	L	E	S	I	A	Y	Q	T	R	D	V	L	E	A	M	Q	A	D	S	G	I	R
Template Known Secondary structure				.	.	.	.							T	S	S	S		T	T							T	T		B		T	T	T		B	T	T										T	T
Template Predicted Secondary structure				.	.	.	.
Template SS confidence
		323	.					.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.
		387	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		P	Q	S	V	T	L	I	G	G	G	A	R	S	E	Y	W	R	Q	M	L	A	D	I	S	G	Q	Q	L	D	Y	R	T	G	G	D	V	G	P	A	L	G	A	A	R	L	A	Q	I	A	A	N	P	E	K	S	L	I	E	L	L	P	Q	L	P	L	E	Q	S	H	L	P	D	A	Q	R	Y	A	A	Y	Q
Query Conservation
Alig confidence																																.	.	.			.																							.	.	.
Template Conservation																																.	.	.			.																							.	.	.
Template Sequence		L	H	A	L	R	V	D	G	G	A	V	A	N	N	F	L	M	Q	F	Q	S	D	I	L	G	T	R	V	E	R	.	.	.	P	E	.	V	T	A	L	G	A	A	Y	L	A	G	L	A	V	G	F	W	Q	N	L	D	E	.	.	.	L	Q	E	I	E	R	E	F	R	P	G	I	E	T	T	E	R	N	Y
Template Known Secondary structure			S						S	G	G	G	G													T	S					.	.	.			.															T	S	S	S					.	.	.		T										S
Template Predicted Secondary structure																																.	.	.			.																							.	.	.
Template SS confidence
		403	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.				.	.		.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.				.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.
		467	.	.	470	.	.	.	.	.	.	.	.	.	480	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		P	R	R	E	T	F	R	R	L	Y	Q	Q	L	L	P	L	M
Query Conservation
Alig confidence			.
Template Conservation			.
Template Sequence		R	.	Y	A	G	W	K	K	A	V	K	R	A	M	A	W	E
Template Known Secondary structure			.												S	S		S
Template Predicted Secondary structure			.
Template SS confidence
		482		.	.	.	.	.	.	.	490	.	.	.	.	.	.	.