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Job DescriptionP16700
Confidence99.82%DateThu Jan 5 11:35:45 GMT 2012
Rank56Aligned Residues303
% Identity20%Templatec3osqA_
PDB info PDB header:fluorescent protein, transport proteinChain: A: PDB Molecule:maltose-binding periplasmic protein, green fluorescent PDBTitle: maltose-bound maltose sensor engineered by insertion of circularly2 permuted green fluorescent protein into e. coli maltose binding3 protein at position 175
Resolution1.90 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   26...30....... ..40.........50.........60.........70.........80.........90.........100...
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Query Sequence  TELLNSSYDVSR. . ELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKLI
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Template Sequence  EGKLVIWINGDKGYNGLAEVGKKFEKD. . . . . TGIKVTVEHPDKLEEKFPQVAATGDGPDIIFW. AHDRFGGYAQSGLLA
Template Known Secondary structure  TTS

TTS
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TTTT
S
S.GGGTT
B
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   4.....10.........20.........30 .........40.........50.........60.. .......70.......
 
   104 .....110.........120......... 130.........140 .........150.........
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Query Sequence  P. . . . . . . . . . . . . . . . . ADWQSRLPNNSSPFYSTMGFLVRKG. NPKNIHDWNDL. . . . . . VRSDVKLIFPNPKTSGNAR
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Template Sequence  EITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFT
Template Known Secondary structure 




TTB
TTT
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TTT
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SBSTTTTT




SS
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   78.80.........90.........100.........110.........120.........130.........140.........150.......
 
   160.........170......
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Query Sequence  YTYLAAWGAADKADGGD. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  WPLIAADGGYAFKYENHLSHNVYIMADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSTQSKLS
Template Known Secondary structure  TT

S


GGGTBTTS
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S
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   158.160.........170.........180.........190.........200.........210.........220.........230.......
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation                                                                                  
Template Sequence  KDPNEKRDHMVLLEFVTAAGITLGMDELYKGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
Template Known Secondary structure 

TT

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TT


TGGGGGSSTTTTTTT
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   238.240.........250.........260.........270.........280.........290.........300.........310.......
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation                                                                                  
Template Sequence  TGKLPVPWPTLVTTLVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKE
Template Known Secondary structure  TSS
SS
GGGGTTT

GGG
B

GGGGGG
TTTT
TTS
TTS

T
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   324.....330.........340.........350.........360.........370.........380.........390.........400...
 
   177..180.........190.........200.........210.........220.......
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . KGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQ
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Template Sequence  DGNILGHKLEYNFNGGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTS
Template Known Secondary structure  TSTTTTT
B
SSSTTTTSSSTTSS
TT

TTS
GGG
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   407..410.........420.........430.........440.........450.........460.........470.........480......
 
   228.230.........240.. .......250.........260.........270....... ..280.........290...
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Query Sequence  YEAQGFEVVIPKTNI. LAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQ. TIITDYYYRVNNPEVM. . . . . . . . . . . .
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Template Sequence  KVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIA
Template Known Secondary structure  T




BBTTB



BTT
S
TTSSS


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   487..490.........500.........510.........520.........530.........540.........550.........560......
 
   294.....300.........310.........320.........330....
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Query Sequence  . . . DKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
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Template Sequence  ATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEDLK
Template Known Secondary structure  S



TTTTSS
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   567..570.........580.........590.........600.........610
 
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No model constructed - rank, confidence too low




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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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