Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.
Sequence		M	T	L	A	Q	F	A	M	T	F	W	H	D	L	A	A	P	I	L	A	G	I	I	T	A	A	I	V	G	W	W	R	N	R	K
Secondary structure
SS confidence
Disorder		?	?																															?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2hg5 chain D Region: 18 - 35 Aligned: 18 Modelled: 18 Confidence: 10.4% Identity: 17% PDB header:membrane protein Chain: D: PDB Molecule:kcsa channel; PDBTitle: cs+ complex of a k channel with an amide to ester substitution in the2 selectivity filter Phyre2 PDB 3t41 chain B Region: 14 - 27 Aligned: 14 Modelled: 14 Confidence: 6.4% Identity: 36% PDB header:hydrolase Chain: B: PDB Molecule:epidermin leader peptide processing serine protease epip; PDBTitle: 1.95 angstrom resolution crystal structure of epidermin leader peptide2 processing serine protease (epip) s393a mutant from staphylococcus3 aureus Phyre2 PDB 1r0r chain E Region: 14 - 27 Aligned: 14 Modelled: 14 Confidence: 6.3% Identity: 29% Fold: Subtilisin-like Superfamily: Subtilisin-like Family: Subtilases Phyre2 PDB 3lpc chain A Region: 14 - 27 Aligned: 14 Modelled: 14 Confidence: 5.7% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:aprb2; PDBTitle: crystal structure of a subtilisin-like protease Phyre2 PDB 1s2n chain B Region: 14 - 27 Aligned: 14 Modelled: 14 Confidence: 5.6% Identity: 29% PDB header:hydrolase Chain: B: PDB Molecule:extracellular subtilisin-like serine proteinase; PDBTitle: crystal strucure of a cold adapted subtilisin-like serine proteinase Phyre2 PDB 2b6n chain A Region: 14 - 27 Aligned: 14 Modelled: 14 Confidence: 5.6% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:proteinase k; PDBTitle: the 1.8 a crystal structure of a proteinase k like enzyme from a2 psychrotroph serratia species Phyre2 PDB 1loi chain A Region: 27 - 35 Aligned: 9 Modelled: 9 Confidence: 5.5% Identity: 56% PDB header:hydrolase Chain: A: PDB Molecule:cyclic 3',5'-amp specific phosphodiesterase rd1; PDBTitle: n-terminal splice region of rat c-amp phosphodiesterase,2 nmr, 7 structures Phyre2 PDB 2iy9 chain A Region: 15 - 27 Aligned: 13 Modelled: 13 Confidence: 5.3% Identity: 31% PDB header:toxin Chain: A: PDB Molecule:suba; PDBTitle: crystal structure of the a-subunit of the ab5 toxin from e.2 coli Phyre2 PDB 1xx7 chain A Region: 6 - 15 Aligned: 10 Modelled: 10 Confidence: 5.3% Identity: 30% Fold: HD-domain/PDEase-like Superfamily: HD-domain/PDEase-like Family: HD domain Phyre2 1 c2hg5D_ 2 c3t41B_ 3 d1r0re_ 4 c3lpcA_ 5 c1s2nB_ 6 c2b6nA_ 7 c1loiA_ 8 c2iy9A_ 9 d1xx7a_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2hg5D_ 10.4 17 PDB header:membrane protein Chain: D: PDB Molecule:kcsa channel; PDBTitle: cs+ complex of a k channel with an amide to ester substitution in the2 selectivity filter 2 c3t41B_ 6.4 36 PDB header:hydrolase Chain: B: PDB Molecule:epidermin leader peptide processing serine protease epip; PDBTitle: 1.95 angstrom resolution crystal structure of epidermin leader peptide2 processing serine protease (epip) s393a mutant from staphylococcus3 aureus 3 d1r0re_ 6.3 29 Fold:Subtilisin-like Superfamily:Subtilisin-like Family:Subtilases 4 c3lpcA_ 5.7 29 PDB header:hydrolase Chain: A: PDB Molecule:aprb2; PDBTitle: crystal structure of a subtilisin-like protease 5 c1s2nB_ 5.6 29 PDB header:hydrolase Chain: B: PDB Molecule:extracellular subtilisin-like serine proteinase; PDBTitle: crystal strucure of a cold adapted subtilisin-like serine proteinase 6 c2b6nA_ 5.6 29 PDB header:hydrolase Chain: A: PDB Molecule:proteinase k; PDBTitle: the 1.8 a crystal structure of a proteinase k like enzyme from a2 psychrotroph serratia species 7 c1loiA_ 5.5 56 PDB header:hydrolase Chain: A: PDB Molecule:cyclic 3',5'-amp specific phosphodiesterase rd1; PDBTitle: n-terminal splice region of rat c-amp phosphodiesterase,2 nmr, 7 structures 8 c2iy9A_ 5.3 31 PDB header:toxin Chain: A: PDB Molecule:suba; PDBTitle: crystal structure of the a-subunit of the ab5 toxin from e.2 coli 9 d1xx7a_ 5.3 30 Fold:HD-domain/PDEase-like Superfamily:HD-domain/PDEase-like Family:HD domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0