Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	Y	R	I	F	L	L	F	F	F	A	L	L	P	T	S	L	V	W	A	A	P	A	Q	R	A	F	S	D	W	Q	V	T	C	N	N	Q	N	F	C	V	A	R	N	T	G	D	H	N	G	L	V	M	T	L	S	R	S	A
Secondary structure
SS confidence
Disorder		?	?	?	?											?	?	?	?	?	?	?	?	?	?	?																									?	?								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	A	H	T	D	A	V	L	R	I	E	R	G	G	L	K	S	P	E	A	S	E	G	E	I	A	P	R	L	L	L	D	G	E	P	L	A	L	S	G	D	K	W	R	I	S	P	W	L	L	V	T	D	D	T	A	T	I	T	A
Secondary structure
SS confidence
Disorder				?												?	?	?	?	?	?	?	?	?	?	?										?	?	?	?	?	?	?	?	?	?	?							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		F	L	Q	M	I	Q	E	G	K	A	I	T	L	R	D	G	D	Q	T	I	S	L	S	G	L	K	A	A	L	L	F	I	D	A	Q	Q	K	R	V	G	S	E	T	A	W	I	K	K	G	D	E	P	P	L	S	V	P	P	A	P
Secondary structure
SS confidence
Disorder								?	?	?					?		?	?	?																																		?				?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	L	K	E	V	A	V	V	N	P	T	P	T	P	L	S	L	E	E	R	N	D	L	L	D	Y	G	N	W	R	M	N	G	L	R	C	S	L	D	P	L	R	R	E	V	N	V	T	A	L	T	D	D	K	A	L	M	M	I	S
Secondary structure
SS confidence
Disorder		?	?	?		?	?	?	?		?	?	?	?	?	?	?	?	?	?	?														?	?		?		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		C	E	A	G	A	Y	N	T	I	D	L	A	W	I	V	S	R	K	K	P	L	A	S	R	P	V	R	L	R	L	P	F	N	N	G	Q	E	T	N	E	L	E	L	M	N	A	T	F	D	E	K	S	R	E	L	V	T	L	A	K
Secondary structure
SS confidence
Disorder																					?	?		?		?				?	?	?	?	?	?	?	?	?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.
Sequence		G	R	G	L	S	D	C	G	I	Q	A	R	W	R	F	D	G	Q	R	F	R	L	V	R	Y	A	A	E	P	T	C	D	N	W	H	G	P	D	A	W	P	T	L	W	I	T	R
Secondary structure
SS confidence
Disorder																								?	?	?	?	?	?	?	?	?	?	?	?										?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3dtd chain I Region: 24 - 156 Aligned: 126 Modelled: 128 Confidence: 98.8% Identity: 20% PDB header:structural genomics, unknown function Chain: I: PDB Molecule:invasion-associated protein b; PDBTitle: crystal structure of invasion associated protein b from bartonella2 henselae Phyre2 PDB 1jke chain A Region: 87 - 172 Aligned: 81 Modelled: 86 Confidence: 30.4% Identity: 12% Fold: DTD-like Superfamily: DTD-like Family: DTD-like Phyre2 PDB 2byc chain A domain 1 Region: 25 - 37 Aligned: 13 Modelled: 13 Confidence: 18.5% Identity: 31% Fold: Ferredoxin-like Superfamily: Acylphosphatase/BLUF domain-like Family: BLUF domain Phyre2 PDB 3ko7 chain E Region: 87 - 172 Aligned: 81 Modelled: 86 Confidence: 18.5% Identity: 12% PDB header:hydrolase Chain: E: PDB Molecule:d-tyrosyl-trna(tyr) deacylase; PDBTitle: dtd from plasmodium falciparum in complex with d-lysine Phyre2 PDB 1j7g chain A Region: 87 - 172 Aligned: 81 Modelled: 86 Confidence: 17.4% Identity: 12% Fold: DTD-like Superfamily: DTD-like Family: DTD-like Phyre2 PDB 2kb2 chain A Region: 25 - 39 Aligned: 15 Modelled: 15 Confidence: 16.3% Identity: 13% PDB header:signaling protein, hydrolase regulator Chain: A: PDB Molecule:blrp1; PDBTitle: blrp1 bluf Phyre2 PDB 2elc chain A domain 1 Region: 91 - 126 Aligned: 36 Modelled: 36 Confidence: 16.2% Identity: 11% Fold: Methionine synthase domain-like Superfamily: Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family: Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Phyre2 PDB 2hfn chain J Region: 25 - 35 Aligned: 11 Modelled: 11 Confidence: 15.8% Identity: 27% PDB header:electron transport Chain: J: PDB Molecule:synechocystis photoreceptor (slr1694); PDBTitle: crystal structures of the synechocystis photoreceptor slr1694 reveal2 distinct structural states related to signaling Phyre2 PDB 2okv chain C Region: 87 - 172 Aligned: 80 Modelled: 86 Confidence: 14.2% Identity: 14% PDB header:hydrolase Chain: C: PDB Molecule:probable d-tyrosyl-trna(tyr) deacylase 1; PDBTitle: c-myc dna unwinding element binding protein Phyre2 PDB 2o8r chain A domain 3 Region: 114 - 133 Aligned: 20 Modelled: 20 Confidence: 14.0% Identity: 25% Fold: Phospholipase D/nuclease Superfamily: Phospholipase D/nuclease Family: Polyphosphate kinase C-terminal domain Phyre2 PDB 1x0p chain A domain 1 Region: 25 - 35 Aligned: 11 Modelled: 11 Confidence: 13.2% Identity: 27% Fold: Ferredoxin-like Superfamily: Acylphosphatase/BLUF domain-like Family: BLUF domain Phyre2 PDB 1yrx chain A domain 1 Region: 25 - 35 Aligned: 11 Modelled: 11 Confidence: 12.0% Identity: 27% Fold: Ferredoxin-like Superfamily: Acylphosphatase/BLUF domain-like Family: BLUF domain Phyre2 PDB 2wjv chain E Region: 150 - 171 Aligned: 22 Modelled: 22 Confidence: 11.3% Identity: 18% PDB header:hydrolase Chain: E: PDB Molecule:regulator of nonsense transcripts 2; PDBTitle: crystal structure of the complex between human nonsense2 mediated decay factors upf1 and upf2 Phyre2 PDB 2dbo chain A Region: 87 - 172 Aligned: 81 Modelled: 86 Confidence: 11.1% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:d-tyrosyl-trna(tyr) deacylase; PDBTitle: crystal structure of d-tyr-trna(tyr) deacylase from aquifex aeolicus Phyre2 PDB 2d32 chain A domain 1 Region: 1 - 16 Aligned: 16 Modelled: 16 Confidence: 10.0% Identity: 19% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase Phyre2 PDB 3nzt chain A Region: 1 - 17 Aligned: 17 Modelled: 17 Confidence: 9.9% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: 2.0 angstrom crystal structure of glutamate--cysteine ligase (gsha)2 ftom francisella tularensis in complex with amp Phyre2 PDB 1uou chain A domain 1 Region: 91 - 126 Aligned: 36 Modelled: 36 Confidence: 9.8% Identity: 22% Fold: Methionine synthase domain-like Superfamily: Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family: Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Phyre2 PDB 1brw chain A domain 1 Region: 91 - 126 Aligned: 36 Modelled: 36 Confidence: 9.1% Identity: 17% Fold: Methionine synthase domain-like Superfamily: Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family: Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Phyre2 PDB 2ov2 chain O Region: 283 - 296 Aligned: 14 Modelled: 14 Confidence: 9.0% Identity: 14% PDB header:protein binding/transferase Chain: O: PDB Molecule:serine/threonine-protein kinase pak 4; PDBTitle: the crystal structure of the human rac3 in complex with the crib2 domain of human p21-activated kinase 4 (pak4) Phyre2 PDB 1khd chain A domain 1 Region: 91 - 126 Aligned: 36 Modelled: 36 Confidence: 8.8% Identity: 6% Fold: Methionine synthase domain-like Superfamily: Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family: Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Phyre2 1 c3dtdI_ 2 d1jkea_ 3 d2byca1 4 c3ko7E_ 5 d1j7ga_ 6 c2kb2A_ 7 d2elca1 8 c2hfnJ_ 9 c2okvC_ 10 d2o8ra3 11 d1x0pa1 12 d1yrxa1 13 c2wjvE_ 14 c2dboA_ 15 d2d32a1 16 c3nztA_ 17 d1uoua1 18 d1brwa1 19 c2ov2O_ 20 d1khda1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3dtdI_ 98.8 20 PDB header:structural genomics, unknown function Chain: I: PDB Molecule:invasion-associated protein b; PDBTitle: crystal structure of invasion associated protein b from bartonella2 henselae 2 d1jkea_ 30.4 12 Fold:DTD-like Superfamily:DTD-like Family:DTD-like 3 d2byca1 18.5 31 Fold:Ferredoxin-like Superfamily:Acylphosphatase/BLUF domain-like Family:BLUF domain 4 c3ko7E_ 18.5 12 PDB header:hydrolase Chain: E: PDB Molecule:d-tyrosyl-trna(tyr) deacylase; PDBTitle: dtd from plasmodium falciparum in complex with d-lysine 5 d1j7ga_ 17.4 12 Fold:DTD-like Superfamily:DTD-like Family:DTD-like 6 c2kb2A_ 16.3 13 PDB header:signaling protein, hydrolase regulator Chain: A: PDB Molecule:blrp1; PDBTitle: blrp1 bluf 7 d2elca1 16.2 11 Fold:Methionine synthase domain-like Superfamily:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain 8 c2hfnJ_ 15.8 27 PDB header:electron transport Chain: J: PDB Molecule:synechocystis photoreceptor (slr1694); PDBTitle: crystal structures of the synechocystis photoreceptor slr1694 reveal2 distinct structural states related to signaling 9 c2okvC_ 14.2 14 PDB header:hydrolase Chain: C: PDB Molecule:probable d-tyrosyl-trna(tyr) deacylase 1; PDBTitle: c-myc dna unwinding element binding protein 10 d2o8ra3 14.0 25 Fold:Phospholipase D/nuclease Superfamily:Phospholipase D/nuclease Family:Polyphosphate kinase C-terminal domain 11 d1x0pa1 13.2 27 Fold:Ferredoxin-like Superfamily:Acylphosphatase/BLUF domain-like Family:BLUF domain 12 d1yrxa1 12.0 27 Fold:Ferredoxin-like Superfamily:Acylphosphatase/BLUF domain-like Family:BLUF domain 13 c2wjvE_ 11.3 18 PDB header:hydrolase Chain: E: PDB Molecule:regulator of nonsense transcripts 2; PDBTitle: crystal structure of the complex between human nonsense2 mediated decay factors upf1 and upf2 14 c2dboA_ 11.1 17 PDB header:hydrolase Chain: A: PDB Molecule:d-tyrosyl-trna(tyr) deacylase; PDBTitle: crystal structure of d-tyr-trna(tyr) deacylase from aquifex aeolicus 15 d2d32a1 10.0 19 Fold:Glutamine synthetase/guanido kinase Superfamily:Glutamine synthetase/guanido kinase Family:Glutamate-cysteine ligase 16 c3nztA_ 9.9 18 PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: 2.0 angstrom crystal structure of glutamate--cysteine ligase (gsha)2 ftom francisella tularensis in complex with amp 17 d1uoua1 9.8 22 Fold:Methionine synthase domain-like Superfamily:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain 18 d1brwa1 9.1 17 Fold:Methionine synthase domain-like Superfamily:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain 19 c2ov2O_ 9.0 14 PDB header:protein binding/transferase Chain: O: PDB Molecule:serine/threonine-protein kinase pak 4; PDBTitle: the crystal structure of the human rac3 in complex with the crib2 domain of human p21-activated kinase 4 (pak4) 20 d1khda1 8.8 6 Fold:Methionine synthase domain-like Superfamily:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain 21 d1v8ga1 not modelled 8.7 11 Fold:Methionine synthase domain-like Superfamily:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain 22 d1u2ca2 not modelled 7.2 29 Fold:Dystroglycan, domain 2 Superfamily:Dystroglycan, domain 2 Family:Dystroglycan, domain 2 23 d2tpta1 not modelled 7.2 23 Fold:Methionine synthase domain-like Superfamily:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain 24 c2odbB_ not modelled 7.2 23 PDB header:protein binding Chain: B: PDB Molecule:serine/threonine-protein kinase pak 6; PDBTitle: the crystal structure of human cdc42 in complex with the crib domain2 of human p21-activated kinase 6 (pak6) 25 d1j26a_ not modelled 6.8 22 Fold:dsRBD-like Superfamily:Peptidyl-tRNA hydrolase domain-like Family:Peptidyl-tRNA hydrolase domain 26 c2o8rA_ not modelled 6.7 24 PDB header:transferase Chain: A: PDB Molecule:polyphosphate kinase; PDBTitle: crystal structure of polyphosphate kinase from2 porphyromonas gingivalis 27 c2bs5A_ not modelled 6.6 28 PDB header:sugar binding protein Chain: A: PDB Molecule:fucose-binding lectin protein; PDBTitle: lectin from ralstonia solanacearum complexed with 2-2 fucosyllactose 28 d1o17a1 not modelled 6.4 8 Fold:Methionine synthase domain-like Superfamily:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain Family:Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain 29 d1f9fa_ not modelled 6.4 15 Fold:Ferredoxin-like Superfamily:Viral DNA-binding domain Family:Viral DNA-binding domain 30 c3ln6A_ not modelled 6.1 31 PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 streptococcus agalactiae 31 d1ut9a1 not modelled 6.1 6 Fold:alpha/alpha toroid Superfamily:Six-hairpin glycosidases Family:Cellulases catalytic domain 32 c3ln7A_ not modelled 6.1 44 PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 pasteurella multocida 33 c1wz5A_ not modelled 6.0 78 PDB header:toxin Chain: A: PDB Molecule:potassium channel blocking toxin 1; PDBTitle: solution structure of pi1-3p 34 c1f3mB_ not modelled 6.0 21 PDB header:transferase Chain: B: PDB Molecule:serine/threonine-protein kinase pak-alpha; PDBTitle: crystal structure of human serine/threonine kinase pak1 35 c1w8xN_ not modelled 5.7 14 PDB header:virus Chain: N: PDB Molecule:protein p31; PDBTitle: structural analysis of prd1 36 c1e0aB_ not modelled 5.6 14 PDB header:signalling protein Chain: B: PDB Molecule:serine/threonine-protein kinase pak-alpha; PDBTitle: cdc42 complexed with the gtpase binding domain of p212 activated kinase

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0