Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	W	A	L	T	A	D	A	D	F	L	A	Q	R	G	Q	G	Q	V	E	Q	V	F	A	R	A	V	N	I	A	L	P	A	R	Q	Q	L	L	T	L	L	C	E	E	Y	D	N	A	P	N	S	C	R	L	A	L	T	H	F	D
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	L	F	R	H	G	D	K	V	Q	F	D	D	Q	G	I	T	V	G	Q	H	L	H	I	E	M	S	R	C	R	R	W	L	S	P	T	L	Q	M	T	A	V	N	F	H	L	I	A	W	L	Q	W	H	D	I	I	H	Q	H	L
Secondary structure
SS confidence
Disorder																															?								?	?	?	?		?	?		?										?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	E	N	E	T	L	F	N	Y	R	G	D	N	P	F	Y	Q	A	L	N	K	E	L	H	I	K	R	R	A	V	I	Q	A	V	N	D	K	Q	N	I	A	S	A	V	A	S	M	M	G	L	G	I	G	L	T	P	S	A	D	D
Secondary structure
SS confidence
Disorder		?	?	?	?				?	?	?	?	?				?															?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Y	L	T	G	L	A	L	I	L	F	I	P	G	H	P	A	E	K	Y	K	E	E	F	Y	L	G	L	Q	R	G	K	N	N	T	T	L	L	S	A	I	T	L	E	A	A	L	Q	Q	R	C	R	E	N	I	H	R	F	I	H	N
Secondary structure
SS confidence
Disorder																													?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.
Sequence		I	I	Y	D	I	P	G	N	A	T	Q	A	I	E	K	I	K	H	I	G	S	S	S	G	C	D	M	L	Y	G	M	A	D	G	C	A	L	S	Q	T	Y	G	G	N	Y	V	S
Secondary structure
SS confidence
Disorder																																												?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1gpr chain A Region: 17 - 79 Aligned: 56 Modelled: 63 Confidence: 62.6% Identity: 20% Fold: Barrel-sandwich hybrid Superfamily: Duplicated hybrid motif Family: Glucose permease-like Phyre2 PDB 2f3g chain A Region: 17 - 79 Aligned: 56 Modelled: 63 Confidence: 58.5% Identity: 18% Fold: Barrel-sandwich hybrid Superfamily: Duplicated hybrid motif Family: Glucose permease-like Phyre2 PDB 1u0m chain A domain 2 Region: 229 - 286 Aligned: 58 Modelled: 58 Confidence: 56.5% Identity: 9% Fold: Thiolase-like Superfamily: Thiolase-like Family: Chalcone synthase-like Phyre2 PDB 1gla chain F Region: 17 - 79 Aligned: 56 Modelled: 63 Confidence: 52.1% Identity: 18% Fold: Barrel-sandwich hybrid Superfamily: Duplicated hybrid motif Family: Glucose permease-like Phyre2 PDB 2gpr chain A Region: 17 - 79 Aligned: 57 Modelled: 63 Confidence: 38.1% Identity: 19% Fold: Barrel-sandwich hybrid Superfamily: Duplicated hybrid motif Family: Glucose permease-like Phyre2 PDB 1pkl chain A domain 1 Region: 33 - 78 Aligned: 46 Modelled: 46 Confidence: 23.5% Identity: 13% Fold: PK beta-barrel domain-like Superfamily: PK beta-barrel domain-like Family: Pyruvate kinase beta-barrel domain Phyre2 PDB 2dch chain X Region: 173 - 188 Aligned: 16 Modelled: 16 Confidence: 23.5% Identity: 50% PDB header:hydrolase Chain: X: PDB Molecule:putative homing endonuclease; PDBTitle: crystal structure of archaeal intron-encoded homing endonuclease i-2 tsp061i Phyre2 PDB 2l55 chain A Region: 15 - 72 Aligned: 50 Modelled: 58 Confidence: 20.8% Identity: 20% PDB header:metal binding protein Chain: A: PDB Molecule:silb,silver efflux protein, mfp component of the three PDBTitle: solution structure of the c-terminal domain of silb from cupriavidus2 metallidurans Phyre2 PDB 1pkm chain A domain 1 Region: 33 - 78 Aligned: 46 Modelled: 46 Confidence: 18.7% Identity: 20% Fold: PK beta-barrel domain-like Superfamily: PK beta-barrel domain-like Family: Pyruvate kinase beta-barrel domain Phyre2 PDB 1ted chain A Region: 229 - 276 Aligned: 48 Modelled: 48 Confidence: 17.2% Identity: 8% Fold: Thiolase-like Superfamily: Thiolase-like Family: Chalcone synthase-like Phyre2 PDB 1ycn chain A Region: 177 - 245 Aligned: 69 Modelled: 69 Confidence: 15.3% Identity: 17% Fold: Annexin Superfamily: Annexin Family: Annexin Phyre2 PDB 1zeq chain X Region: 15 - 72 Aligned: 50 Modelled: 58 Confidence: 15.2% Identity: 20% PDB header:metal binding protein Chain: X: PDB Molecule:cation efflux system protein cusf; PDBTitle: 1.5 a structure of apo-cusf residues 6-88 from escherichia2 coli Phyre2 PDB 1e0t chain A domain 1 Region: 29 - 78 Aligned: 50 Modelled: 50 Confidence: 15.0% Identity: 18% Fold: PK beta-barrel domain-like Superfamily: PK beta-barrel domain-like Family: Pyruvate kinase beta-barrel domain Phyre2 PDB 1apj chain A Region: 162 - 180 Aligned: 19 Modelled: 19 Confidence: 13.6% Identity: 26% Fold: TB module/8-cys domain Superfamily: TB module/8-cys domain Family: TB module/8-cys domain Phyre2 PDB 2g50 chain A domain 1 Region: 33 - 78 Aligned: 46 Modelled: 46 Confidence: 13.1% Identity: 20% Fold: PK beta-barrel domain-like Superfamily: PK beta-barrel domain-like Family: Pyruvate kinase beta-barrel domain Phyre2 PDB 1liu chain A domain 1 Region: 33 - 78 Aligned: 46 Modelled: 46 Confidence: 12.9% Identity: 11% Fold: PK beta-barrel domain-like Superfamily: PK beta-barrel domain-like Family: Pyruvate kinase beta-barrel domain Phyre2 PDB 1u0m chain A Region: 229 - 276 Aligned: 48 Modelled: 48 Confidence: 12.7% Identity: 8% PDB header:biosynthetic protein Chain: A: PDB Molecule:putative polyketide synthase; PDBTitle: crystal structure of 1,3,6,8-tetrahydroxynaphthalene synthase (thns)2 from streptomyces coelicolor a3(2): a bacterial type iii polyketide3 synthase (pks) provides insights into enzymatic control of reactive4 polyketide intermediates Phyre2 PDB 3ale chain B Region: 229 - 286 Aligned: 57 Modelled: 58 Confidence: 11.3% Identity: 12% PDB header:transferase Chain: B: PDB Molecule:os07g0271500 protein; PDBTitle: a type iii polyketide synthase that produces diarylheptanoid Phyre2 PDB 1ksq chain A Region: 162 - 179 Aligned: 18 Modelled: 18 Confidence: 11.0% Identity: 22% Fold: TB module/8-cys domain Superfamily: TB module/8-cys domain Family: TB module/8-cys domain Phyre2 PDB 2d1k chain C Region: 175 - 185 Aligned: 11 Modelled: 11 Confidence: 10.5% Identity: 36% PDB header:structural protein Chain: C: PDB Molecule:metastasis suppressor protein 1; PDBTitle: ternary complex of the wh2 domain of mim with actin-dnase i Phyre2 1 d1gpra_ 2 d2f3ga_ 3 d1u0ma2 4 d1glaf_ 5 d2gpra_ 6 d1pkla1 7 c2dchX_ 8 c2l55A_ 9 d1pkma1 10 d1teda_ 11 d1ycna_ 12 c1zeqX_ 13 d1e0ta1 14 d1apja_ 15 d2g50a1 16 d1liua1 17 c1u0mA_ 18 c3aleB_ 19 d1ksqa_ 20 c2d1kC_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1gpra_ 62.6 20 Fold:Barrel-sandwich hybrid Superfamily:Duplicated hybrid motif Family:Glucose permease-like 2 d2f3ga_ 58.5 18 Fold:Barrel-sandwich hybrid Superfamily:Duplicated hybrid motif Family:Glucose permease-like 3 d1u0ma2 56.5 9 Fold:Thiolase-like Superfamily:Thiolase-like Family:Chalcone synthase-like 4 d1glaf_ 52.1 18 Fold:Barrel-sandwich hybrid Superfamily:Duplicated hybrid motif Family:Glucose permease-like 5 d2gpra_ 38.1 19 Fold:Barrel-sandwich hybrid Superfamily:Duplicated hybrid motif Family:Glucose permease-like 6 d1pkla1 23.5 13 Fold:PK beta-barrel domain-like Superfamily:PK beta-barrel domain-like Family:Pyruvate kinase beta-barrel domain 7 c2dchX_ 23.5 50 PDB header:hydrolase Chain: X: PDB Molecule:putative homing endonuclease; PDBTitle: crystal structure of archaeal intron-encoded homing endonuclease i-2 tsp061i 8 c2l55A_ 20.8 20 PDB header:metal binding protein Chain: A: PDB Molecule:silb,silver efflux protein, mfp component of the three PDBTitle: solution structure of the c-terminal domain of silb from cupriavidus2 metallidurans 9 d1pkma1 18.7 20 Fold:PK beta-barrel domain-like Superfamily:PK beta-barrel domain-like Family:Pyruvate kinase beta-barrel domain 10 d1teda_ 17.2 8 Fold:Thiolase-like Superfamily:Thiolase-like Family:Chalcone synthase-like 11 d1ycna_ 15.3 17 Fold:Annexin Superfamily:Annexin Family:Annexin 12 c1zeqX_ 15.2 20 PDB header:metal binding protein Chain: X: PDB Molecule:cation efflux system protein cusf; PDBTitle: 1.5 a structure of apo-cusf residues 6-88 from escherichia2 coli 13 d1e0ta1 15.0 18 Fold:PK beta-barrel domain-like Superfamily:PK beta-barrel domain-like Family:Pyruvate kinase beta-barrel domain 14 d1apja_ 13.6 26 Fold:TB module/8-cys domain Superfamily:TB module/8-cys domain Family:TB module/8-cys domain 15 d2g50a1 13.1 20 Fold:PK beta-barrel domain-like Superfamily:PK beta-barrel domain-like Family:Pyruvate kinase beta-barrel domain 16 d1liua1 12.9 11 Fold:PK beta-barrel domain-like Superfamily:PK beta-barrel domain-like Family:Pyruvate kinase beta-barrel domain 17 c1u0mA_ 12.7 8 PDB header:biosynthetic protein Chain: A: PDB Molecule:putative polyketide synthase; PDBTitle: crystal structure of 1,3,6,8-tetrahydroxynaphthalene synthase (thns)2 from streptomyces coelicolor a3(2): a bacterial type iii polyketide3 synthase (pks) provides insights into enzymatic control of reactive4 polyketide intermediates 18 c3aleB_ 11.3 12 PDB header:transferase Chain: B: PDB Molecule:os07g0271500 protein; PDBTitle: a type iii polyketide synthase that produces diarylheptanoid 19 d1ksqa_ 11.0 22 Fold:TB module/8-cys domain Superfamily:TB module/8-cys domain Family:TB module/8-cys domain 20 c2d1kC_ 10.5 36 PDB header:structural protein Chain: C: PDB Molecule:metastasis suppressor protein 1; PDBTitle: ternary complex of the wh2 domain of mim with actin-dnase i 21 c3oitB_ not modelled 10.1 13 PDB header:transferase Chain: B: PDB Molecule:os07g0271500 protein; PDBTitle: crystal structure of curcuminoid synthase cus from oryza sativa 22 c3ov3A_ not modelled 9.4 17 PDB header:transferase Chain: A: PDB Molecule:curcumin synthase; PDBTitle: g211f mutant of curcumin synthase 1 from curcuma longa 23 d1a3xa1 not modelled 9.1 9 Fold:PK beta-barrel domain-like Superfamily:PK beta-barrel domain-like Family:Pyruvate kinase beta-barrel domain 24 c2p0uB_ not modelled 9.0 10 PDB header:transferase Chain: B: PDB Molecule:stilbenecarboxylate synthase 2; PDBTitle: crystal structure of marchantia polymorpha stilbenecarboxylate2 synthase 2 (stcs2) 25 c2e28A_ not modelled 8.7 20 PDB header:transferase Chain: A: PDB Molecule:pyruvate kinase; PDBTitle: crystal structure analysis of pyruvate kinase from bacillus2 stearothermophilus 26 d1u0ua2 not modelled 8.6 11 Fold:Thiolase-like Superfamily:Thiolase-like Family:Chalcone synthase-like 27 c3mmlE_ not modelled 8.3 18 PDB header:hydrolase Chain: E: PDB Molecule:allophanate hydrolase subunit 2; PDBTitle: allophanate hydrolase complex from mycobacterium smegmatis, msmeg0435-2 msmeg0436 28 c3u1nC_ not modelled 8.1 19 PDB header:hydrolase Chain: C: PDB Molecule:sam domain and hd domain-containing protein 1; PDBTitle: structure of the catalytic core of human samhd1 29 c3a5qA_ not modelled 7.6 13 PDB header:transferase Chain: A: PDB Molecule:benzalacetone synthase; PDBTitle: benzalacetone synthase from rheum palmatum 30 d1fasa_ not modelled 7.1 57 Fold:Snake toxin-like Superfamily:Snake toxin-like Family:Snake venom toxins 31 c3t07D_ not modelled 7.0 15 PDB header:transferase/transferase inhibitor Chain: D: PDB Molecule:pyruvate kinase; PDBTitle: crystal structure of s. aureus pyruvate kinase in complex with a2 naturally occurring bis-indole alkaloid 32 c2h84A_ not modelled 7.0 15 PDB header:biosynthetic protein, transferase Chain: A: PDB Molecule:steely1; PDBTitle: crystal structure of the c-terminal type iii polyketide synthase (pks2 iii) domain of 'steely1' (a type i/iii pks hybrid from dictyostelium) 33 d1m98a1 not modelled 6.9 9 Fold:Orange carotenoid protein, N-terminal domain Superfamily:Orange carotenoid protein, N-terminal domain Family:Orange carotenoid protein, N-terminal domain 34 d1ve2a1 not modelled 6.8 47 Fold:Tetrapyrrole methylase Superfamily:Tetrapyrrole methylase Family:Tetrapyrrole methylase 35 d1twda_ not modelled 6.7 100 Fold:TIM beta/alpha-barrel Superfamily:CutC-like Family:CutC-like 36 c1ee0A_ not modelled 6.5 6 PDB header:transferase Chain: A: PDB Molecule:2-pyrone synthase; PDBTitle: 2-pyrone synthase complexed with acetoacetyl-coa 37 d1cnza_ not modelled 6.5 33 Fold:Isocitrate/Isopropylmalate dehydrogenase-like Superfamily:Isocitrate/Isopropylmalate dehydrogenase-like Family:Dimeric isocitrate & isopropylmalate dehydrogenases 38 c3iwpK_ not modelled 6.4 80 PDB header:metal binding protein Chain: K: PDB Molecule:copper homeostasis protein cutc homolog; PDBTitle: crystal structure of human copper homeostasis protein cutc 39 c2q14A_ not modelled 6.4 19 PDB header:hydrolase Chain: A: PDB Molecule:phosphohydrolase; PDBTitle: crystal structure of phosphohydrolase (bt4208) from bacteroides2 thetaiotaomicron vpi-5482 at 2.20 a resolution 40 c1m98A_ not modelled 6.3 9 PDB header:unknown function Chain: A: PDB Molecule:orange carotenoid protein; PDBTitle: crystal structure of orange carotenoid protein 41 d1h9ma1 not modelled 5.9 22 Fold:OB-fold Superfamily:MOP-like Family:BiMOP, duplicated molybdate-binding domain 42 c2e0kA_ not modelled 5.9 41 PDB header:transferase Chain: A: PDB Molecule:precorrin-2 c20-methyltransferase; PDBTitle: crystal structure of cbil, a methyltransferase involved in anaerobic2 vitamin b12 biosynthesis 43 d1guta_ not modelled 5.9 14 Fold:OB-fold Superfamily:MOP-like Family:Molybdate/tungstate binding protein MOP 44 c3nutC_ not modelled 5.5 41 PDB header:transferase Chain: C: PDB Molecule:precorrin-3 methylase; PDBTitle: crystal structure of the methyltransferase cobj 45 c3eoeC_ not modelled 5.5 12 PDB header:transferase Chain: C: PDB Molecule:pyruvate kinase; PDBTitle: crystal structure of pyruvate kinase from toxoplasma gondii, 55.m00007 46 d1drsa_ not modelled 5.5 38 Fold:Snake toxin-like Superfamily:Snake toxin-like Family:Dendroaspin 47 c2bb3B_ not modelled 5.3 22 PDB header:transferase Chain: B: PDB Molecule:cobalamin biosynthesis precorrin-6y methylase (cbie); PDBTitle: crystal structure of cobalamin biosynthesis precorrin-6y methylase2 (cbie) from archaeoglobus fulgidus 48 c2j5dA_ not modelled 5.2 21 PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: nmr structure of bnip3 transmembrane domain in lipid2 bicelles

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0