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Job DescriptionP21517
Confidence100.00%DateThu Jan 5 11:38:27 GMT 2012
Rank5Aligned Residues530
% Identity17%Templatec2e8yA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:amyx protein; PDBTitle: crystal structure of pullulanase type i from bacillus subtilis str.2 168
Resolution2.11 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   3......10.........20.........30.........40.........50.........60.........70.........80..
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Query Sequence  NAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDNEEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFK
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Template Sequence  DEFYYDGELGAVYTADHTVFKVWAPAATSAAVKLS. . HPNKSGRTFQMTR. . . . . . LEKGVYAVTVT. . GDLHGYEYLFC
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   98.100.........110.........120.........130.. .......140..... ....150...... ...160.......
 
   83......90 .........100.........110 .........120.........130.........140.........150.....
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Query Sequence  LLWHDRQR. WFTPQGFSRMPPARLEQFAV. . . . . . DVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQE
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Template Sequence  ICNNSEWMETVDQYAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINK. . . . . . . . . . .
Template Known Secondary structure  TT

TT
SSB
GGG
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GGG


STTS

SST...........
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   168.170.........180.........190.........200.........210.........220.........230......
 
   156...160.........170.........180..... ....190.........200....... ..210....
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Query Sequence  IILRDWDEPVTAQAGGSTFYGGDLDGISEK. . . . . . . . . . . LPYLKKLGVTALYLNPVFKAPS. . . . . . . . . . VHKYDTE
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . GKYLALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPL
Template Known Secondary structure  .....................TSGGGGG
TT
B
TTS
B
T
SS

SSS
TTSGGG





S
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   237..240.........250.........260.........270.........280.........290.....
 
   215....220..... ....230.........240.........250..... ....260.........270.........280.....
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Query Sequence  DYRHVDPQFGG. . . . . . . . DGALLRLRHNTQQLGMRLVLDGVFNHSGDS. HAWFDRHNRGTGGACHNPESPWRDWYSFSD
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Template Sequence  HFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPGY. . . . . . . . . . . . . FFRHDE
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STTSS
SSSTT

TT

SSGGGSSTTT.............SB
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   296...300.........310.........320.........330.........340.........350...... ...360..
 
   286...290.........300.........310.........320.........330.........340.........350.........360.....
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Query Sequence  DGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEAAKETQ
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Template Sequence  CGKPSNGTGVGN. . DIASERRMARKFIADC. . . . VVYWLEE. YNVDGFRFDLLGILD. . . . . . . . IDTVLYMKEKATKAK
Template Known Secondary structure  TSSB

TTSSS
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   363......370.... .....380.........390 ....... ..400.........410.. .......420.......
 
   366...370.........380.........390... ......400.........410... ......420.........430...
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Query Sequence  PEAYIVGEHFGDARQWLQADVEDAAMNY. . . . . RGFTFPLWGFLANTDISYDP. . . . . . QQIDAQTCMAWMDNYRAGLS.
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Template Sequence  PGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLKATGFALGNGESAQAVMHGIAGSSGWKAL
Template Known Secondary structure  TT







SS
GGGB

GGGGGG
TT



SSSTT


GGGT
GGGBTTS
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   428.430.........440.........450.........460.........470.........480.........490.........500.......
 
   434.....440.........450....... ..460.........470.........480.........490.. ...
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Query Sequence  . . . HQQQLRMFNQLDSHDTARFKTLLG. . . . . . . . . RDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKND. . . . . . PFC
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Template Sequence  APIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQRLAVAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDS
Template Known Secondary structure 

SSSSGGG
S

SSSS

TT

TSBSTTGGGT


TT
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   508.510.........520.........530.........540.........550.........560.........570.........580.......
 
   496...500.........510.........520.........530. ........540.........550 .........560...
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Query Sequence  RKPFPWQVEKQDTALFALYQRMIALRKKSQALRHGG. . . . . . . CQVLYAEDNVVVFVRVLNQ. . . . . QRVLVAINRGEAC
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Template Sequence  INQLDWDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHLIAYRLYDLDEVDEWKDIIVIHHASPDS
Template Known Secondary structure  S

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GGGG

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   588.590.........600.........610.........620.........630.........640.........650.........660.......
 
   564 .....570 .........580.........590.........600..
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Query Sequence  E. VVLPAS. PFLNAVQWQCKEGHGQLTDGILALPAISATVW
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Template Sequence  VEWRLPNDIPYRLLCDPSGFQEDPTEIKKTVAVNGIGTVIL
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SS
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   668.670.........680.........690.........700........
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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