Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	E	K	N	N	E	V	I	Q	T	H	P	L	V	G	W	D	I	S	T	V	D	S	Y	D	A	L	M	L	R	L	H	Y	Q	T	P	N	K	S	E	Q	E	G	T	E	V	G	Q	T	L	W	L	T	T	D	V	A	R	Q	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																											?		?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.
Sequence		I	S	I	L	E	A	G	I	A	K	I	E	S	G	D	F	Q	V	N	E	Y	R	R	H
Secondary structure
SS confidence
Disorder													?				?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1vko chain A domain 1 Region: 15 - 27 Aligned: 13 Modelled: 13 Confidence: 28.0% Identity: 54% Fold: NAD(P)-binding Rossmann-fold domains Superfamily: NAD(P)-binding Rossmann-fold domains Family: Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain Phyre2 PDB 1p1j chain A domain 1 Region: 15 - 27 Aligned: 13 Modelled: 13 Confidence: 20.3% Identity: 54% Fold: NAD(P)-binding Rossmann-fold domains Superfamily: NAD(P)-binding Rossmann-fold domains Family: Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain Phyre2 PDB 1vko chain A Region: 15 - 27 Aligned: 13 Modelled: 13 Confidence: 18.4% Identity: 54% PDB header:isomerase Chain: A: PDB Molecule:inositol-3-phosphate synthase; PDBTitle: crystal structure of inositol-3-phosphate synthase (ce21227) from2 caenorhabditis elegans at 2.30 a resolution Phyre2 PDB 1pv0 chain A Region: 58 - 77 Aligned: 20 Modelled: 20 Confidence: 12.8% Identity: 30% Fold: Long alpha-hairpin Superfamily: Sporulation inhibitor Sda Family: Sporulation inhibitor Sda Phyre2 PDB 1p1h chain D Region: 15 - 27 Aligned: 13 Modelled: 13 Confidence: 11.8% Identity: 54% PDB header:isomerase Chain: D: PDB Molecule:inositol-3-phosphate synthase; PDBTitle: crystal structure of the 1l-myo-inositol/nad+ complex Phyre2 PDB 3bj4 chain B Region: 37 - 63 Aligned: 27 Modelled: 27 Confidence: 11.6% Identity: 37% PDB header:signaling protein Chain: B: PDB Molecule:potassium voltage-gated channel subfamily kqt PDBTitle: the kcnq1 (kv7.1) c-terminus, a multi-tiered scaffold for2 subunit assembly and protein interaction Phyre2 PDB 2j44 chain A domain 2 Region: 28 - 37 Aligned: 10 Modelled: 10 Confidence: 11.2% Identity: 30% Fold: Prealbumin-like Superfamily: Starch-binding domain-like Family: PUD-like Phyre2 PDB 2lfe chain A Region: 13 - 52 Aligned: 38 Modelled: 40 Confidence: 11.2% Identity: 34% PDB header:ligase Chain: A: PDB Molecule:e3 ubiquitin-protein ligase hecw2; PDBTitle: solution nmr structure of n-terminal domain of human e3 ubiquitin-2 protein ligase hecw2, northeast structural genomics consortium (nesg)3 target ht6306a Phyre2 PDB 2le2 chain B Region: 21 - 29 Aligned: 9 Modelled: 9 Confidence: 9.0% Identity: 78% PDB header:hydrolase inhibitor Chain: B: PDB Molecule:p56; PDBTitle: novel dimeric structure of phage phi29-encoded protein p56: insights2 into uracil-dna glycosylase inhibition Phyre2 PDB 2j43 chain A domain 2 Region: 28 - 38 Aligned: 11 Modelled: 11 Confidence: 7.4% Identity: 27% Fold: Prealbumin-like Superfamily: Starch-binding domain-like Family: PUD-like Phyre2 PDB 2qbx chain B Region: 10 - 54 Aligned: 38 Modelled: 45 Confidence: 6.6% Identity: 37% PDB header:signaling protein Chain: B: PDB Molecule:ephrin type-b receptor 2; PDBTitle: ephb2/snew antagonistic peptide complex Phyre2 1 d1vkoa1 2 d1p1ja1 3 c1vkoA_ 4 d1pv0a_ 5 c1p1hD_ 6 c3bj4B_ 7 d2j44a2 8 c2lfeA_ 9 c2le2B_ 10 d2j43a2 11 c2qbxB_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1vkoa1 28.0 54 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain 2 d1p1ja1 20.3 54 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain 3 c1vkoA_ 18.4 54 PDB header:isomerase Chain: A: PDB Molecule:inositol-3-phosphate synthase; PDBTitle: crystal structure of inositol-3-phosphate synthase (ce21227) from2 caenorhabditis elegans at 2.30 a resolution 4 d1pv0a_ 12.8 30 Fold:Long alpha-hairpin Superfamily:Sporulation inhibitor Sda Family:Sporulation inhibitor Sda 5 c1p1hD_ 11.8 54 PDB header:isomerase Chain: D: PDB Molecule:inositol-3-phosphate synthase; PDBTitle: crystal structure of the 1l-myo-inositol/nad+ complex 6 c3bj4B_ 11.6 37 PDB header:signaling protein Chain: B: PDB Molecule:potassium voltage-gated channel subfamily kqt PDBTitle: the kcnq1 (kv7.1) c-terminus, a multi-tiered scaffold for2 subunit assembly and protein interaction 7 d2j44a2 11.2 30 Fold:Prealbumin-like Superfamily:Starch-binding domain-like Family:PUD-like 8 c2lfeA_ 11.2 34 PDB header:ligase Chain: A: PDB Molecule:e3 ubiquitin-protein ligase hecw2; PDBTitle: solution nmr structure of n-terminal domain of human e3 ubiquitin-2 protein ligase hecw2, northeast structural genomics consortium (nesg)3 target ht6306a 9 c2le2B_ 9.0 78 PDB header:hydrolase inhibitor Chain: B: PDB Molecule:p56; PDBTitle: novel dimeric structure of phage phi29-encoded protein p56: insights2 into uracil-dna glycosylase inhibition 10 d2j43a2 7.4 27 Fold:Prealbumin-like Superfamily:Starch-binding domain-like Family:PUD-like 11 c2qbxB_ 6.6 37 PDB header:signaling protein Chain: B: PDB Molecule:ephrin type-b receptor 2; PDBTitle: ephb2/snew antagonistic peptide complex

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0