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Job DescriptionP76616
Confidence100.00%DateThu Jan 5 12:25:01 GMT 2012
Rank34Aligned Residues370
% Identity14%Templatec2qpuB_
PDB info PDB header:hydrolaseChain: B: PDB Molecule:alpha-amylase type a isozyme; PDBTitle: sugar tongs mutant s378p in complex with acarbose
Resolution1.70 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   217..220...... ...230.........240.........250.........260.........270.........280.. .....
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Query Sequence  EAILHAFNAT. . . . . . . . YQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHN. PMGDL
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Template Sequence  QVLFQGFNWESWKQSGGWYNMMMGKVDDIAAAGVTHVWLPPPSHSVSN. . . . . . . . . . . . . EGYMPGRLYDIDASKYGNA
Template Known Secondary structure 




TTGGG
TT
GGGTT




S
BSST.............TS
SB

TT
GGG
SS

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   2.......10.........20.........30.........40......... 50.........60........
 
   288.290.........300.........310.........320.........330.........340.........350.........360.......
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Query Sequence  QDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIH
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Template Sequence  AELKSLIGALHGKGVQAIADIVINHRCADYKDSRGIYCIFEGGTSDGR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  TT


SB


S
TTS



SSSS
T................................
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   6970.........80.........90.........100.........110......
 
   368.370.........380.........390.........400.........410.........420...... ...430.........440......
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Query Sequence  PEDIHLTAKDFEASKDNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMR. KYGVRGLRYDAAKHSKHEQI
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Template Sequence  . . . . . . LDWGPHMICRDDTKYSDGTANLDTGADFAAAPDIDHLNDRVQRELKEWLLWLKSDLGFDAWRLDFARGYSPEMA
Template Known Secondary structure  ......T


GGGB
TT
TTT

S
S


SS


TTS
BB
TTSTT


TTGGGS
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   117..120.........130.........140.........150.........160.........170.........180.........190
 
   447..450.........460.........470.........480.........490....... ..500.........510
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Query Sequence  ERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLL. . . . . . . . . . . . . . . . YERDDLSAIDFSL
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Template Sequence  KVYIDGT. . . . . . . . . . . . . . . . . . . . SPSLAVAEVWDNMATGGDGKPNYDQDAHRQNLVNWVDKVGGAASAGMVFDFTT
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S







B
TTS
B
S

TGGGSS
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   191...... ..200.........210.........220.........230.........240.........250
 
   511........520.........530.........540.........550.........560.........570.........580.........590
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Query Sequence  LMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYIAALPFSRPLVYWD
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Template Sequence  KGILNAAVEGELWRLIDPQGKAPGVMGWWPAKAVTFVDNHDTGSTQAMWP. . . . FPSDKVMQGYAYILTHP. GIPCIFYD
Template Known Secondary structure  TTT
GGGSS
TTSS
SSGGGT
GGGS

TTTSTTT
SS
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GGGTTSS.S
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   251........260.........270.........280.........290.........300 .........310....... ..320.....
 
   591........600.........610.........620.........630.........640.........650.........660.........670
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Query Sequence  GQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVSATKNVLAFSRGDSVNINHSPHDG
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Template Sequence  HFFNW. . . . . . . . . . . . . . . . . . . . . . GFKDQIAALVAIRK. . RNGITATSALKILMHEGDAYVAEIDGK. . . . . . . . . V
Template Known Secondary structure  TS......................S
..TT

TT

TTTTT.........
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   326...330 .........340.... .....350.........360.........370. .
 
   671........680.........690.........700.
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Query Sequence  LVIINKGNEEVEGTWPNKLQPGIYKNMGSNS
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Template Sequence  VVKIGPRYDV. . . . . . GAVIPAGFVTSAHGN
Template Known Secondary structure  SS



......GGGS
TT
T
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   373......380.. .......390.......
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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