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Job DescriptionP76616
Confidence100.00%DateThu Jan 5 12:25:01 GMT 2012
Rank46Aligned Residues565
% Identity13%Templatec2e8yA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:amyx protein; PDBTitle: crystal structure of pullulanase type i from bacillus subtilis str.2 168
Resolution2.11 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   52.......60..... ....70.........80.........90.........100.........110.........120...
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Query Sequence  PQGLKDREIISTFH. . . . . . . . AEKVINDLLKNDYKISLSPDTTYRELNKAAQRSITAPDRIGERKTWVYQRDTMIERGD
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Template Sequence  ELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEKGV. . . . . . . . . . . . . . . . . . . . . . . YA. . . . VTVTG
Template Known Secondary structure 


SS
TT
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TTS



GGG...........................S
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   105....110.........120.........130.........140.........150 .. .....
 
   124.....130.........140.........150...... ...160.........170.........180.........190.........200..
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Query Sequence  NSGVYQYGRAEHFTHIISDKPSPKDKYVAYAIN. IPDYELAADVYNINVTSPSGQQETFKILINLEHLRQTLERKSLTAV
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Template Sequence  DLHGYEYLFCICNNSEWMETVDQYAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENS. . . GMI
Template Known Secondary structure 

TT
TT

TT
SSB
GGG
S















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GGG


STTS...

S
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   158.160.........170.........180.........190.........200.........210.........220.........230. ...
 
   203......210.........220.........230.........240.........250.........260.........270.........280..
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Query Sequence  QKSQCEIITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHN
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Template Sequence  NKGKYLALTETDTQT. . . . . . . . . ANGSSSGLAYVKELGVTHVELLPVNDFAGV. DEEKPLDAYNW. . GYNPLHFFAPEG
Template Known Secondary structure  STTSGGGGG
TT
B
.........TTS
B
T
SS

SSS.
TTSGGG



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S
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   235....240......... 250.........260.........270......... 280.........290 .........300..
 
   283... ...290.........300.........310.........320.........330.........340.........350....
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Query Sequence  PMGD. . . . . . . . LQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIEGGIPGYWQGQVLIPF
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Template Sequence  SYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPG. . . . . YFFRHDECGKPSNGTGV. . . . .
Template Known Secondary structure  TTSS
SSSTT

TT

SSGGGSSTT.....TSB
B
TTSSB

TTSS.....
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   303......310.........320.........330.........340.........350..... ....360.........370..
 
   355....360.........370.........380.........390.........400.........410.........420...... ...430...
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Query Sequence  IKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMR. KYGVRGL
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GNDIASERRMARKFIADCVVYWLEEYNVDGF
Template Known Secondary structure  .................................................S

B
TTS


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   373......380.........390.........400...
 
   434.....440.........450.........460.........470.........480.........490.........500......
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Query Sequence  RYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAI. . . . . . .
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Template Sequence  RFDLLGILDIDTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLKATGF
Template Known Secondary structure  TTGGGSBSTT







SS
GGGB

GGGGGG
TT



SSSTT


G
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   404.....410.........420.........430.........440.........450.........460.........470.........480...
 
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Query Sequence  . . . DFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH. . . . . QQDEQLAMAYIA
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Template Sequence  ALGNGESAQAVMHGIAGSSGW. . . . . . KALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQRLAVAIIL
Template Known Secondary structure  GGT
GGGBTTS
B......TTB

SSSSGGG
S

SSSS

TT

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   484.....490.........500.... .....510.........520.........530.........540.........550.......
 
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Query Sequence  ALPFSRPLVYWDGQVLKSTTEIKNYDGS. . . . . TRVGGEAWLNKGCSTYQQLYNEFHAL. . . . . YIDKAGIWSAFEGVSA
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Template Sequence  LAQ. GVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTL
Template Known Secondary structure  TSB.STTGGGT


TT
S

TT

S

T
GGGG

S
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   558.560 .........570.........580.........590.........600.........610.........620.........630......
 
   649650.........660.........670.........680.........690.........700.........710.........720........
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Query Sequence  TKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNI
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Template Sequence  KEHLIAYRLYDLDE. VDEWKDIIVIHHASPDSVEWRLP. . . NDIPYRLLCDPSGF. . . . . . . . . . . . QEDPTEIKK. . TV
Template Known Secondary structure 
SS


TT.T
SSS
SSS
S...S
S
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   637..640.........650 .........660.........670... ......680....... ..690...... ..
 
   729730.........740..
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Query Sequence  NIPGRSALLLGKTG
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Template Sequence  AVNGIGTVILYLAS
Template Known Secondary structure  SS

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   699700.........710..
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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