Return to main results Retrieve Phyre Job Id

Job DescriptionP76616
Confidence100.00%DateThu Jan 5 12:25:01 GMT 2012
Rank21Aligned Residues525
% Identity14%Templatec2d0gA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha-amylase i; PDBTitle: crystal structure of thermoactinomyces vulgaris r-47 alpha-2 amylase 1 (tvai) mutant d356n/e396q complexed with p5, a3 pullulan model oligosaccharide
Resolution2.60 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   93......100.........110.........120.........130.........140.........150.........160..... ....
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Query Sequence  KAAQRSITAPDRIGERKTWVYQRDTMIERGDNSGVYQYGRAEHFTHIISDKPSPKDKYVAYAINIPDYELAAD. . . VYNI
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Template Sequence  HWVPMVWDSNDPTGTFDYWKGT. . . . IPASPSIKYYRF. . . . . . . . . . . . QINDGTSTAWYNGNGPSSTEPNADDFYIIP
Template Known Secondary structure 

TTSS....


SS
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SS


T
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   5960.........70.........80 .........90.. .......100.........110.........120..
 
   170.........180.........190.........200...... ...210.........220.........230.........
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Query Sequence  NVTSPSGQQETFKILINLEHLRQTLERKSLTAVQKSQ. . . . . . . . . . CEIITPKKPGEAILHAFNATYQQIRENMSEFAR
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Template Sequence  NFKTPDWLKNGVMYQIFPDRFYNGDSSNDVQTGSYTYNGTPTEKKAWGSSVYADPGYDNSLVFFGGDLAGIDQKLGYIKK
Template Known Secondary structure  T






GGGT

S
GGG


TTS
BSSS
B


TTS

S

TTS
GGG



TT
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   123......130.........140.........150.........160.........170.........180.........190.........200..
 
   240.........250.........260.........270.........280.........290.........300. ........310....
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Query Sequence  . CHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFG. . . . IDIIPDYTFNFMG
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Template Sequence  TLGANILYLNPIFKAPTN. . . . . . . . . . . . . HKYDTQDYMAVDPAFGDNSTLQTLINDIHSTANGPKGYLILDGVFNHTG
Template Known Secondary structure  TT


S


SSS.............S

S
S
TTT


SSSSS



SB

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   203......210.........220 .........230.........240.........250.........260.........
 
   315....320.........330.........340.........350.........360.........370.........380.........390....
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Query Sequence  IGGSGKNDLDYPSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENLH
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Template Sequence  DSHPWFDK. . . . . . . . . . . . . . . . . . . . . . . YNNFSSQGAYESQSSPWYNYYTFYTWPDSYASFLGF. . . . . . . . . . . . .
Template Known Secondary structure  TTSTTT
T.......................T

SSS

TTT
TT
TTGGGB
TTT
TT.............
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   270....... ..280.........290.........300.........310...
 
   395....400.........410. ........420 ...... ...430.........440... ......450.....
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Query Sequence  ALKEKRLNGMPKTTPKS. . DQVIMLQNQ. . . . YVREMR. . KYGVRGLRYDAAKHSKH. . . . . . . . . . . EQIERSITPPLK
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Template Sequence  . . . . . . . NSLPKLNYGNSGSAVRGVIYNNSNSVAKTYLNPPYSVDGWRLNAAQYVDANGNNGSDVTNHQIWSEFRNAVKG
Template Known Secondary structure  .......BB


STT
TTSTTSSTTT


TTGGG

STT


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   314.....320.........330.........340.........350.........360.........370.........380......
 
   456...460.........470.........480.........490.........500.........510.........520.. ....
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Query Sequence  NYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGG. . . . . . . . . DLQT
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Template Sequence  VNS. . . . . . . . . . . . . . . . NAAIIGQYWGNAN. . . . . . PWTAQGNQWDAATNFDGFTQPVSEWITGKDYQNNSASISTTQ
Template Known Secondary structure 
T................T


SS

G......GGTTTSSS
SB
TTTTSB
TTS
B



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   387.. 390.........400.. .......410.........420.........430.........440....
 
   527..530.........540.. .......550.........560.........570.........580.........590.........600...
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Query Sequence  LASKPGSTISSIPSER. . . RILININHDFPNNGNLFNDFLFNHQQDEQLAMAYIAALPFSRPLVYWDGQVLKSTTEIKNY
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Template Sequence  FDSWLRGTRANYPTNVQQSMMNFLSNHDITRFATRSG. . . . GDLWKTYLALIFQMTYV. GTPTIYYGDEYGMQ. GGADPD
Template Known Secondary structure  TTS
TT
S

TTS

TT....T
TTSS.STTTTTT

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STTT
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   445....450.........460.........470.........480. ........490........ .500.........510.. ......
 
   604.....610.........620.........630.........640.........650.........660.........670.........680...
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Query Sequence  DGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVSATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG
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Template Sequence  NRRSFDWSQATPSNSAVALTQKLITIRNQYPALRTGSFMTLITDDTNKIYSYGR. . . . . . FDNVNRIAVVLNNDSVSHTV
Template Known Secondary structure  TS



TTTSSTTS
STTTT......
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   519520.........530.........540.........550.........560.........570.. .......580.........590..
 
   684..... 690.........700.........710.........720.........730.........
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Query Sequence  TWPNKL. . . . QPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLG
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Template Sequence  NVPVWQLSMPNGSTVTDKIT. . . . . . . . . . . . . . . . GHSYTVQNGMVTVAVDGHYGAVLA
Template Known Secondary structure 
GGGGT

TT
TTT................


BTTB
TT
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   593......600.........610.. .......620.........630......
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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