Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	E	T	T	V	F	L	S	N	R	S	Q	A	V	R	L	P	K	A	V	A	L	P	E	N	V	K	R	V	E	V	I	A	V	G	R	T	R	I	I	T	P	A	G	E	T	W	D	E	W	F	D	G	N	S	V	S	A	D	F
Secondary structure
SS confidence
Disorder		?	?																																											?	?	?							?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.
Sequence		M	D	N	R	E	Q	P	G	M	Q	E	R	E	S	F
Secondary structure
SS confidence
Disorder			?		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3tnd chain F Region: 2 - 67 Aligned: 66 Modelled: 66 Confidence: 100.0% Identity: 97% PDB header:translation, toxin Chain: F: PDB Molecule:antitoxin vapb; PDBTitle: crystal structure of shigella flexneri vapbc toxin-antitoxin complex Phyre2 PDB 3zvk chain G Region: 2 - 75 Aligned: 72 Modelled: 74 Confidence: 100.0% Identity: 32% PDB header:antitoxin/toxin/dna Chain: G: PDB Molecule:antitoxin of toxin-antitoxin system vapb; PDBTitle: crystal structure of vapbc2 from rickettsia felis bound to2 a dna fragment from their promoter Phyre2 PDB 1ub4 chain C Region: 1 - 55 Aligned: 53 Modelled: 55 Confidence: 98.9% Identity: 28% Fold: Double-split beta-barrel Superfamily: AbrB/MazE/MraZ-like Family: Kis/PemI addiction antidote Phyre2 PDB 1mvf chain D Region: 3 - 43 Aligned: 41 Modelled: 41 Confidence: 97.6% Identity: 29% Fold: Double-split beta-barrel Superfamily: AbrB/MazE/MraZ-like Family: Kis/PemI addiction antidote Phyre2 PDB 1una chain A Region: 8 - 34 Aligned: 27 Modelled: 27 Confidence: 15.1% Identity: 30% Fold: RNA bacteriophage capsid protein Superfamily: RNA bacteriophage capsid protein Family: RNA bacteriophage capsid protein Phyre2 PDB 2fy9 chain A domain 1 Region: 14 - 42 Aligned: 27 Modelled: 29 Confidence: 14.0% Identity: 19% Fold: Double-split beta-barrel Superfamily: AbrB/MazE/MraZ-like Family: AbrB N-terminal domain-like Phyre2 PDB 1uf0 chain A Region: 26 - 54 Aligned: 29 Modelled: 29 Confidence: 10.5% Identity: 21% Fold: beta-Grasp (ubiquitin-like) Superfamily: Doublecortin (DC) Family: Doublecortin (DC) Phyre2 PDB 1yfb chain A domain 1 Region: 14 - 46 Aligned: 31 Modelled: 33 Confidence: 9.6% Identity: 13% Fold: Double-split beta-barrel Superfamily: AbrB/MazE/MraZ-like Family: AbrB N-terminal domain-like Phyre2 PDB 3ila chain G Region: 17 - 45 Aligned: 23 Modelled: 29 Confidence: 8.3% Identity: 26% PDB header:signaling protein Chain: G: PDB Molecule:ryanodine receptor 1; PDBTitle: crystal structure of rabbit ryanodine receptor 1 n-terminal domain (9-2 205) Phyre2 PDB 2qc8 chain J Region: 8 - 17 Aligned: 10 Modelled: 10 Confidence: 8.2% Identity: 50% PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of human glutamine synthetase in complex with adp2 and methionine sulfoximine phosphate Phyre2 PDB 2d3a chain J Region: 8 - 20 Aligned: 13 Modelled: 13 Confidence: 6.7% Identity: 31% PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the maize glutamine synthetase2 complexed with adp and methionine sulfoximine phosphate Phyre2 PDB 1fr5 chain A Region: 8 - 34 Aligned: 27 Modelled: 27 Confidence: 6.7% Identity: 26% Fold: RNA bacteriophage capsid protein Superfamily: RNA bacteriophage capsid protein Family: RNA bacteriophage capsid protein Phyre2 PDB 2hjq chain A domain 2 Region: 3 - 14 Aligned: 12 Modelled: 12 Confidence: 6.5% Identity: 33% Fold: GINS/PriA/YqbF domain Superfamily: PriA/YqbF domain Family: YqbF N-terminal domain-like Phyre2 PDB 1mjd chain A Region: 23 - 54 Aligned: 32 Modelled: 32 Confidence: 6.3% Identity: 16% Fold: beta-Grasp (ubiquitin-like) Superfamily: Doublecortin (DC) Family: Doublecortin (DC) Phyre2 PDB 2bu1 chain A domain 1 Region: 8 - 34 Aligned: 27 Modelled: 27 Confidence: 6.3% Identity: 30% Fold: RNA bacteriophage capsid protein Superfamily: RNA bacteriophage capsid protein Family: RNA bacteriophage capsid protein Phyre2 PDB 2w1t chain B Region: 14 - 46 Aligned: 31 Modelled: 33 Confidence: 6.2% Identity: 16% PDB header:transcription Chain: B: PDB Molecule:stage v sporulation protein t; PDBTitle: crystal structure of b. subtilis spovt Phyre2 PDB 1mg4 chain A Region: 27 - 54 Aligned: 28 Modelled: 28 Confidence: 6.0% Identity: 21% Fold: beta-Grasp (ubiquitin-like) Superfamily: Doublecortin (DC) Family: Doublecortin (DC) Phyre2 PDB 2a6q chain A domain 1 Region: 25 - 54 Aligned: 30 Modelled: 30 Confidence: 5.7% Identity: 3% Fold: YefM-like Superfamily: YefM-like Family: YefM-like Phyre2 PDB 3bmv chain A domain 3 Region: 29 - 46 Aligned: 18 Modelled: 18 Confidence: 5.6% Identity: 6% Fold: Glycosyl hydrolase domain Superfamily: Glycosyl hydrolase domain Family: alpha-Amylases, C-terminal beta-sheet domain Phyre2 PDB 1v5r chain A domain 1 Region: 20 - 54 Aligned: 33 Modelled: 35 Confidence: 5.2% Identity: 9% Fold: N domain of copper amine oxidase-like Superfamily: GAS2 domain-like Family: GAS2 domain Phyre2 1 c3tndF_ 2 c3zvkG_ 3 d1ub4c_ 4 d1mvfd_ 5 d1unaa_ 6 d2fy9a1 7 d1uf0a_ 8 d1yfba1 9 c3ilaG_ 10 c2qc8J_ 11 c2d3aJ_ 12 d1fr5a_ 13 d2hjqa2 14 d1mjda_ 15 d2bu1a1 16 c2w1tB_ 17 d1mg4a_ 18 d2a6qa1 19 d3bmva3 20 d1v5ra1 21 22

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3tndF_ 100.0 97 PDB header:translation, toxin Chain: F: PDB Molecule:antitoxin vapb; PDBTitle: crystal structure of shigella flexneri vapbc toxin-antitoxin complex 2 c3zvkG_ 100.0 32 PDB header:antitoxin/toxin/dna Chain: G: PDB Molecule:antitoxin of toxin-antitoxin system vapb; PDBTitle: crystal structure of vapbc2 from rickettsia felis bound to2 a dna fragment from their promoter 3 d1ub4c_ 98.9 28 Fold:Double-split beta-barrel Superfamily:AbrB/MazE/MraZ-like Family:Kis/PemI addiction antidote 4 d1mvfd_ 97.6 29 Fold:Double-split beta-barrel Superfamily:AbrB/MazE/MraZ-like Family:Kis/PemI addiction antidote 5 d1unaa_ 15.1 30 Fold:RNA bacteriophage capsid protein Superfamily:RNA bacteriophage capsid protein Family:RNA bacteriophage capsid protein 6 d2fy9a1 14.0 19 Fold:Double-split beta-barrel Superfamily:AbrB/MazE/MraZ-like Family:AbrB N-terminal domain-like 7 d1uf0a_ 10.5 21 Fold:beta-Grasp (ubiquitin-like) Superfamily:Doublecortin (DC) Family:Doublecortin (DC) 8 d1yfba1 9.6 13 Fold:Double-split beta-barrel Superfamily:AbrB/MazE/MraZ-like Family:AbrB N-terminal domain-like 9 c3ilaG_ 8.3 26 PDB header:signaling protein Chain: G: PDB Molecule:ryanodine receptor 1; PDBTitle: crystal structure of rabbit ryanodine receptor 1 n-terminal domain (9-2 205) 10 c2qc8J_ 8.2 50 PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of human glutamine synthetase in complex with adp2 and methionine sulfoximine phosphate 11 c2d3aJ_ 6.7 31 PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the maize glutamine synthetase2 complexed with adp and methionine sulfoximine phosphate 12 d1fr5a_ 6.7 26 Fold:RNA bacteriophage capsid protein Superfamily:RNA bacteriophage capsid protein Family:RNA bacteriophage capsid protein 13 d2hjqa2 6.5 33 Fold:GINS/PriA/YqbF domain Superfamily:PriA/YqbF domain Family:YqbF N-terminal domain-like 14 d1mjda_ 6.3 16 Fold:beta-Grasp (ubiquitin-like) Superfamily:Doublecortin (DC) Family:Doublecortin (DC) 15 d2bu1a1 6.3 30 Fold:RNA bacteriophage capsid protein Superfamily:RNA bacteriophage capsid protein Family:RNA bacteriophage capsid protein 16 c2w1tB_ 6.2 16 PDB header:transcription Chain: B: PDB Molecule:stage v sporulation protein t; PDBTitle: crystal structure of b. subtilis spovt 17 d1mg4a_ 6.0 21 Fold:beta-Grasp (ubiquitin-like) Superfamily:Doublecortin (DC) Family:Doublecortin (DC) 18 d2a6qa1 5.7 3 Fold:YefM-like Superfamily:YefM-like Family:YefM-like 19 d3bmva3 5.6 6 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 20 d1v5ra1 5.2 9 Fold:N domain of copper amine oxidase-like Superfamily:GAS2 domain-like Family:GAS2 domain 21 c3r2cJ_ not modelled 5.2 27 PDB header:transcription/rna Chain: J: PDB Molecule:30s ribosomal protein s10; PDBTitle: crystal structure of antitermination factors nusb and nuse in complex2 with boxa rna 22 c1pgsA_ not modelled 5.2 19 PDB header:endoglycosidase Chain: A: PDB Molecule:peptide-n(4)-(n-acetyl-beta-d-glucosaminyl) PDBTitle: the three-dimensional structure of pngase f, a2 glycosylasparaginase from flavobacterium meningosepticum

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0