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Job DescriptionP0AG80
Confidence100.00%DateThu Jan 5 11:28:23 GMT 2012
Rank5Aligned Residues367
% Identity22%Templatec3osqA_
PDB info PDB header:fluorescent protein, transport proteinChain: A: PDB Molecule:maltose-binding periplasmic protein, green fluorescent PDBTitle: maltose-bound maltose sensor engineered by insertion of circularly2 permuted green fluorescent protein into e. coli maltose binding3 protein at position 175
Resolution1.90 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   23......30.........40.........50.........60.........70.........80.........90.........100..
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Query Sequence  AVTTIPFWHSMEGELGKEVDSLAQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
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Template Sequence  EEGKLVIWINGDKGY. NGLAEVGKKFEKD. TGIKVTVE. . . HPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLA
Template Known Secondary structure 
TTS

TTS
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TTTT
S
SGGGTT
B
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   3......10....... ..20.........30 ........ .40.........50.........60.........70.......
 
   103......110.........120.........130.........140.........150.........160.........170.........180..
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Query Sequence  PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGLDPEQPPKTWQDLADYAAKLKAS
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Template Sequence  EITP. . . . . . . . DKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN. . . . . . . PPKTWEEIPALDKELKAK
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TTB
TTT
S

TTT
SS.......

SBSTTTT
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   78.80. ........90.........100.........110.........120.... .....130.........140..
 
   183......190.........200.........
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Query Sequence  GMKCGYASGWQGWIQLENFSAWNGLPF. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  G. KSALMFNLQEPYFTWPLIAADGGYAFKYENHLSHNVYIMADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
Template Known Secondary structure  T.




SSTT

S


GGGTBTTS
SSSS
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   143 ......150.........160.........170.........180.........190.........200.........210.........220.
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation                                                                                  
Template Sequence  VLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEG
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S

TT

SS




TT


TGGGGGSSTT
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   222.......230.........240.........250.........260.........270.........280.........290.........300.
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation                                                                                  
Template Sequence  EGDATYGKLTLKFICTTGKLPVPWPTLVTTLVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEG
Template Known Secondary structure  TTTTTTSS
SS
GGGGTTT

GGG
B

GGGGGG
TTTT
TTS
T
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   308.310.........320.........330.........340.........350.........360.........370.........380.......
 
   210.........220.........230.........240.........250.........260....
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . ASKNNGFDGTDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDC
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Template Sequence  DTLVNRIELKGIDFKEDGNILGHKLEYNFNGGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
Template Known Secondary structure  TS

TTSTTTTT
B
SSSTTTTSSSTTSS
TT

TTS
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   391........400.........410.........420.........430.........440.........450.........460.........470
 
   265....270.........280.........290.........300.........310.........320.........330.........340....
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Query Sequence  AMTTASSGSLANIREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKPENAAEWHQKT
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Template Sequence  AMTINGPWAWSNIDT. SKVNYGVTVLPTFK. . . GQPSKPFVGVLSAGINAA. . SPNKELAKEFLENYLLTDEGLEAVNKD
Template Known Secondary structure 
GGG.T




BBT...TB



BTT..
S
TTSS
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   471........480..... ....490......... 500.........510....... ..520.........530.........540....
 
   345....350.........360.........370.........380.........390.........400.........410.........420....
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Query Sequence  GYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGNMPQIRVIVDEELESVWTGKKTPQQALDTAVER
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Template Sequence  KPLGAVALKSYEEELV. . . . KDPRIAATMENAQKGEIMP. . . . . NIPQMSAFWYAVRTAVINAASGRQTVDEDLKDAQTR
Template Known Secondary structure  S


SBT....TSS

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TTTTSS
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   545....550.........560 .........570......... 580.........590.........600.........610.....
 
   425
Predicted Secondary structure 
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Query Sequence  G
Query Conservation   
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Template Conservation 
Template Sequence  I
Template Known Secondary structure 
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Template SS confidence 
   616
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
© Structural Bioinformatics Group, Imperial College, London
Lawrence Kelley, Benjamin Jefferys 
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