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Job DescriptionP31133
Confidence100.00%DateThu Jan 5 11:47:16 GMT 2012
Rank4Aligned Residues332
% Identity20%Templatec3osqA_
PDB info PDB header:fluorescent protein, transport proteinChain: A: PDB Molecule:maltose-binding periplasmic protein, green fluorescent PDBTitle: maltose-bound maltose sensor engineered by insertion of circularly2 permuted green fluorescent protein into e. coli maltose binding3 protein at position 175
Resolution1.90 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   28.30.........40.. .......50.........60.........70.........80.........90.........100....
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Query Sequence  EQKTLHIYNWSDYIA. . . PDTVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLDKS
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Template Sequence  EEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITP.
Template Known Secondary structure 
TTS

TTS





TTTT
S
SGGGTT
B




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   3......10.........20.........30.........40.........50.........60.........70.........80.
 
   105....110.........120.........130.........140.........150.........160.........170. ........180...
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Query Sequence  KLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSWDLILKPENLEK. LKSCGVSFLDAP
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Template Sequence  DKAFQDKLYPFTWDAVRY. . NGKLIAYPIAVEALSLIYNKDLLPN. . . . . . PPKTWEEIPALDKELKAKGKSALMFNLQE
Template Known Secondary structure 
TTB
T..TT
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TTT
SS......

SBSTTTTT




SS
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   82.......90......... 100.........110.........120.... .....130.........140.........150...
 
   184.....190.........200...
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Query Sequence  EEVFATVLNYLGKDPNSTKA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  PYFTWPLIAADGGYAFKYENHLSHNVYIMADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSTQ
Template Known Secondary structure  TT

S


GGGTBTTS
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S
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   154.....160.........170.........180.........190.........200.........210.........220.........230...
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
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Template Sequence  SKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
Template Known Secondary structure 

TT

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TT


TGGGGGSSTTTTTT
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   234.....240.........250.........260.........270.........280.........290.........300.........310...
 
  
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Template Sequence  FICTTGKLPVPWPTLVTTLVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGI
Template Known Secondary structure  TTSS
SS
GGGGTTT

GGG
B

GGGGGG
TTTT
TTS
TT
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   320.........330.........340.........350.........360.........370.........380.........390.........
 
   204.....210.........220.. .......230.........240.........250
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . DDYTGPATDLLLKLRPNIR. . . YFHSSQYINDLANGDICVAIGWAGDVWQ
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Template Sequence  DFKEDGNILGHKLEYNFNGGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSN
Template Known Secondary structure  S

TTSTTTTT
B
SSSTTTTSSSTTSS
TT

TTS
GGG
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   403......410.........420.........430.........440.........450.........460.........470.........480..
 
   251........260.........270.........280.........290.........300.........310.........320.........330
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Query Sequence  ASNRAKEAKNGVNVSFSIPKEGAMAFFDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVR
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Template Sequence  IDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSY. . EEELV
Template Known Secondary structure  T




BBTTB



BTT
S
TTSSS


SB..T
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   483......490.........500.........510.........520.........530.........540.........550..... ....560
 
   331........340.........350.. .......360.........370
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Query Sequence  ENPGIYPPADVRAKLFTLKVQD. . PKIDRVRTRAWTKVKSGK
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Template Sequence  KDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGR
Template Known Secondary structure  TSS



TTTTS
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   561........570.........580.........590.........600..
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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