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Job DescriptionP76041
Confidence100.00%DateThu Jan 5 12:17:40 GMT 2012
Rank17Aligned Residues431
% Identity18%Templatec2d0gA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha-amylase i; PDBTitle: crystal structure of thermoactinomyces vulgaris r-47 alpha-2 amylase 1 (tvai) mutant d356n/e396q complexed with p5, a3 pullulan model oligosaccharide
Resolution2.60 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   37..40.........50.........60... ......70.......
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Query Sequence  TQRRKKHWDESDVVLITYADQFHSNDL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . KPLPTFNQFYHQWL
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Template Sequence  PNFKTPDWLKNGVMYQIFPDRFYNGDSSNDVQTGSYTYNGTPTEKKAWGSSVYADPGYDNSLVFFGGDLAGIDQKLGYIK
Template Known Secondary structure  TT






GGGT

S
GGG


TTS
BSSS
B


TTS

S

TTS
GGG



T
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   122.......130.........140.........150.........160.........170.........180.........190.........200.
 
   78 .80.........90.........100.........110.........120.... .....130.........140.......
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Query Sequence  Q. SIFSHVHLLPFYPWSSDDGFSVIDYHQVASEAGEWQDIQQLGECSH. . . . . . . . . LMFDFVCNHMSAKSEWFKNYLQQ
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Template Sequence  KTLGANILYLNPIFKAPTNHKYDTQDYMAVDPAFGDNSTLQTLINDIHSTANGPKGYLILDGVFNHTGDSHPWFDKY. . .
Template Known Secondary structure  TTT


S


SSSS

S
S
TTT


SSSSS



SB

TTSTTT
TT...
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   202.......210.........220.........230.........240.........250.........260.........270........
 
   148.150.........160.........170.........180.........190.........200.... .....210...... ...
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Query Sequence  HPGFEDFFIAVDPQTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRS. . PEVLLAMVDVLL. . . . . CYL.
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Template Sequence  . . . . . . . . . . . . NNFSSQGAYESQSSPWYNYYTFYTWPDSYASFLGFNSLPKLNYGNSGSAVRGVIYNNSNSVAKTYLNP
Template Known Secondary structure  ............

SSS

TTT
TT
TTGGGB
TTT
TTBB


STT
TTSTTSST
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   279280.........290.........300.........310.........320.........330.........340......
 
   220.........230.........240.........250.........260.........270.........280.........290.........
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Query Sequence  AKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLP
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Template Sequence  PYSVDGWRLNAAQYVDANGNN. . GSDVTNHQIWSEFRNAVKGVNSNAAIIGQYWGNANPWT. . . . AQGNQWDAATNFDGF
Template Known Secondary structure  TT


TTGGG

STT

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SS
TT


SS

GGGT....TTSSS
SB
TTTT
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   347..350.........360....... ..370.........380.........390.........400..... ....410.........420
 
   300......... 310.........320.........330 .........340.........350.........360......
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Query Sequence  PLVLHAVQKQ. . . . . . . . . . NVEALCAWAQNLTLPSSNTTW. . . FNFLASHDGIGLNPLRGLLPESEILELVEALQQEGA
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Template Sequence  TQPVSEWITGKDYQNNSASISTTQFDSWLRGTRANYPTNVQQSMMNFLSNHDITRFATRSG. . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  SB
TTS
B



TTS
TT
S

TTS

TT...................
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   421........430.........440.........450.........460.........470.........480.
 
   367..370.........380.........390.........400.........410.........420.........430.........440......
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Query Sequence  LVNWKNNPDGTRSPYEINVTYMDALSRRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAIN
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GDLWKTYLALIFQMTYVGTPTIYYGDEYGMQ. . . . . . . . . GGADPDN
Template Known Secondary structure  .................................T
TTSSSTTTTTT

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STTTT
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   482.......490.........500.........510.. .......
 
   447..450.........460.........470.........480.........490........ .500.........510.........520.....
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Query Sequence  RKKYHSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTIN. SSVMRIPRSNADGNCLTGLFNVSKNIQ
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Template Sequence  RRSFDWSQ. . . . . . . . ATPSNSAVALTQKLITIRNQYPALRTGSFMTLITDDTNKIYSYGRFD. NVNRIAVVLNNDSVSH
Template Known Secondary structure  S



TTT........SSTTS
STTTT
.SS

SSS
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   520....... ..530.........540.........550.........560.........570.... .....580.........590
 
   526...530... ......540....... ..550........
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Query Sequence  HVNITNLH. . . . . . . . GRDLISEVDILGNE. . . . ITLRPWQVMWI
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Template Sequence  TVNVPVWQLSMPNGSTVTDKITGHSYTVQNGMVTVAVDGHYGAVL
Template Known Secondary structure 
GGGGT

TT
TTT


BTTB
TT
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   591........600.........610.........620.........630.....
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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