Job Description P76041 Confidence 100.00% Date Thu Jan 5 12:17:40 GMT 2012 Rank 1 Aligned Residues 546 % Identity 27% Template c1jgiA_ PDB info PDB header:transferase Chain: A: PDB Molecule:amylosucrase; PDBTitle: crystal structure of the active site mutant glu328gln of2 amylosucrase from neisseria polysaccharea in complex with3 the natural substrate sucrose Resolution 2.00 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.										.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40		.	.	.	.	.	.			.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.
Predicted Secondary structure																			.	.	.	.	.	.	.	.	.																							.							.	.
Query SS confidence																			.	.	.	.	.	.	.	.	.																							.							.	.
Query Sequence		K	Q	K	I	T	D	Y	L	D	E	I	Y	G	G	T	F	T	.	.	.	.	.	.	.	.	.	A	T	H	L	Q	K	L	V	T	R	L	E	S	A	K	R	L	I	T	Q	R	R	.	K	K	H	W	D	E	.	.	S	D	V	V	L	I	T	Y	A	D	Q	F	H	S	N	D	L	K	P	L	P	T	F
Query Conservation																			.	.	.	.	.	.	.	.	.																							.							.	.
Alig confidence																			.	.	.	.	.	.	.	.	.																							.							.	.														.	.	.	.
Template Conservation																																																																								.	.	.	.
Template Sequence		F	P	K	L	M	N	E	L	D	S	V	Y	G	N	N	E	A	L	L	P	M	L	E	M	L	L	A	Q	A	W	Q	S	Y	S	Q	R	N	S	S	L	K	D	I	D	I	A	R	E	N	N	P	D	W	I	L	S	N	K	Q	V	G	G	V	C	Y	V	D	L	F	A	.	.	.	.	G	D	L	K	G	L
Template Known Secondary structure														T	T		T	T																			S															G	G	G	G	G		T	T													.	.	.	.	S	S
Template Predicted Secondary structure																																																																								.	.	.	.
Template SS confidence
		40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.					110	.	.	.	.	.
		70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.				.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.						.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.
Predicted Secondary structure																									.	.	.																																	.	.	.	.	.
Query SS confidence																									.	.	.																																	.	.	.	.	.
Query Sequence		N	Q	F	Y	H	Q	W	L	Q	S	I	F	S	H	V	H	L	L	P	F	Y	P	W	.	.	.	S	S	D	D	G	F	S	V	I	D	Y	H	Q	V	A	S	E	A	G	E	W	Q	D	I	Q	Q	L	G	E	C	S	H	.	.	.	.	.	L	M	F	D	F	V	C	N	H	M	S	A	K	S	E	W	F
Query Conservation																									.	.	.																																	.	.	.	.	.
Alig confidence																									.	.	.																																	.	.	.	.	.
Template Conservation
Template Sequence		K	D	K	I	P	Y	F	Q	E	L	G	L	T	Y	L	Y	L	M	P	L	F	K	C	P	E	G	K	S	D	G	G	Y	A	V	S	S	Y	R	D	V	N	P	A	L	G	T	I	G	D	L	R	E	V	I	A	A	L	H	E	A	G	I	S	A	V	V	D	F	I	F	N	H	T	S	N	E	H	E	W	A
Template Known Secondary structure				T								T		S								B				S	S		S	T	T	T	T	S		S		S	S	S	B		T	T	T																T	T										S				T	T	S
Template Predicted Secondary structure
Template SS confidence
		116	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.
		142	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.					.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.
Predicted Secondary structure																								.	.	.	.
Query SS confidence																								.	.	.	.
Query Sequence		K	N	Y	L	Q	Q	H	P	G	F	E	D	F	F	I	A	V	D	P	Q	T	D	.	.	.	.	L	S	A	V	T	R	P	R	A	L	P	L	L	T	P	F	Q	M	R	D	H	S	T	R	H	L	W	T	T	F	S	D	D	Q	I	D	L	N	Y	R	S	P	E	V	L	L	A	M	V	D	V	L	L	C
Query Conservation																								.	.	.	.
Alig confidence																								.	.	.	.																						.
Template Conservation																																																	.
Template Sequence		Q	R	C	A	A	G	D	P	L	F	D	N	F	Y	Y	I	F	P	D	R	R	M	P	D	Q	Y	D	R	T	L	R	E	I	F	P	D	Q	H	P	G	G	F	S	Q	L	E	D	.	G	R	W	V	W	T	T	F	N	S	F	Q	W	D	L	N	Y	S	N	P	W	V	F	R	A	M	A	G	E	M	L	F
Template Known Secondary structure							T		G	G	G	T	T	S	B				S	S	S								T	S				S		T	T	T		S	T	T					T	T	.	S						S	S		T	T				B		T	T	S
Template Predicted Secondary structure																																																	.
Template SS confidence
		196	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.		.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.
		218	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		Y	L	A	K	G	A	E	Y	V	R	L	D	A	V	G	F	M	W	K	E	P	G	T	S	C	I	H	L	E	K	T	H	L	I	I	K	L	L	R	S	I	I	D	N	V	A	P	G	T	V	I	I	T	E	T	N	V	P	H	K	D	N	I	A	Y	F	G	A	G	D	D	E	A	H	M	V	Y	Q	F	S
Query Conservation
Alig confidence																																																																					.	.
Template Conservation																																																																					.	.
Template Sequence		L	A	N	L	G	V	D	I	L	R	M	D	A	V	A	F	I	W	K	Q	M	G	T	S	C	E	N	L	P	Q	A	H	A	L	I	R	A	F	N	A	V	M	R	I	A	A	P	A	V	F	F	K	S	Q	A	I	V	H	P	D	Q	V	V	Q	Y	I	G	.	.	Q	D	E	C	Q	I	G	Y	N	P	L
Template Known Secondary structure				T	T	T								T	G	G	G	S				T	T	S		S	S	S																				T	T									S						G	G	G	B	S	.	.	T	T	S	B	S
Template Predicted Secondary structure																																																																					.	.
Template SS confidence
		275	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.			.	.	.	.	.	.	.	.	350	.	.
		298	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		L	P	P	L	V	L	H	A	V	Q	K	Q	N	V	E	A	L	C	A	W	A	Q	N	L	T	L	P	S	S	N	T	T	W	F	N	F	L	A	S	H	D	G	I	G	L	N	P	L	R	G	L	L	P	E	S	E	I	L	E	L	V	E	A	L	Q	Q	E	G	A	L	V	N	W	K	N	N	P	D	G	T
Query Conservation
Alig confidence
Template Conservation
Template Sequence		Q	M	A	L	L	W	N	T	L	A	T	R	E	V	N	L	L	H	Q	A	L	T	Y	R	H	N	L	P	E	H	T	A	W	V	N	Y	V	R	S	H	D	D	I	G	W	T	F	A	D	E	D	A	A	Y	L	G	I	S	G	Y	D	H	R	Q	F	L	N	R	F	F	V	N	R	F	D	G	S	F	A	R
Template Known Secondary structure																									S					T	T							S			S	S		B												T	T															T	T		S	T	T
Template Predicted Secondary structure
Template SS confidence
		353	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.
		378	.	380	.	.	.	.	.	.	.	.	.	390									.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.				.	.	.	.	.	.	.	440	.	.	.	.	.	.
Predicted Secondary structure															.	.	.	.	.	.	.	.																																											.	.	.
Query SS confidence															.	.	.	.	.	.	.	.																																											.	.	.
Query Sequence		R	S	P	Y	E	I	N	V	T	Y	M	D	A	.	.	.	.	.	.	.	.	L	S	R	R	E	S	S	D	E	E	R	C	A	R	F	I	L	A	H	A	I	L	L	S	F	P	G	V	P	A	I	Y	I	Q	S	I	L	G	S	R	N	D	.	.	.	Y	A	G	V	E	K	L	G	Y	N	R	A	I	N
Query Conservation															.	.	.	.	.	.	.	.																																											.	.	.
Alig confidence															.	.	.	.	.	.	.	.																																											.	.	.
Template Conservation
Template Sequence		G	V	P	F	Q	Y	N	P	S	T	G	D	C	R	V	S	G	T	A	A	A	L	V	G	L	A	Q	D	D	P	H	A	V	D	R	I	K	L	L	Y	S	I	A	L	S	T	G	G	L	P	L	I	Y	L	G	D	E	V	G	T	L	N	D	D	D	W	S	Q	D	S	N	K	S	D	D	S	R	W	A	H
Template Known Secondary structure									T	T	T														T	T	T	T	T		T	T															S	S	S						T	T	G	G	G	T					S	S	G	G	G		T	T	T	T	T		G	G	G	G	G
Template Predicted Secondary structure
Template SS confidence
		433	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480	.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.
		447	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480	.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		R	K	K	Y	H	S	K	E	I	T	R	E	L	N	D	E	A	T	L	R	H	A	V	Y	H	E	L	S	R	L	I	T	L	R	R	S	H	N	E	F	H	P	D	N	N	F	T	I	D	T	I	N	S	S	V	M	R	I	P	R	S	N	A	D	G	N	C	L	T	G	L	F	N	V	S	K	N	I	Q	H
Query Conservation
Alig confidence												.																																																		.	.	.	.
Template Conservation												.																																																		.	.	.	.
Template Sequence		R	P	R	Y	N	E	A	L	Y	A	.	Q	R	N	D	P	S	T	A	A	G	Q	I	Y	Q	D	L	R	H	M	I	A	V	R	Q	S	N	P	R	F	D	G	G	R	L	V	T	F	N	T	N	N	K	H	I	I	G	Y	I	R	.	.	.	.	N	N	A	L	L	A	F	G	N	F	S	E	Y	P	Q	T
Template Known Secondary structure											T	.	T	T	T		T	T	S																				G	G	G		S	S								S		T	T							.	.	.	.	T	T	T								S	S	S
Template Predicted Secondary structure												.																																																		.	.	.	.
Template SS confidence
		513	.	.	.	.	.	.	520	.	.		.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540	.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.					.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.
		527	.	.	530						.	.	.	.	.	.	.	.	.	540	.	.	.		.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.
Predicted Secondary structure						.	.	.	.	.														.
Query SS confidence						.	.	.	.	.														.
Query Sequence		V	N	I	T	.	.	.	.	.	N	L	H	G	R	D	L	I	S	E	V	D	I	.	L	G	N	E	I	T	L	R	P	W	Q	V	M	W	I	K
Query Conservation						.	.	.	.	.														.
Alig confidence						.	.	.	.	.														.
Template Conservation
Template Sequence		V	T	A	H	T	L	Q	A	M	P	F	K	A	H	D	L	I	G	G	K	T	V	S	L	N	Q	D	L	T	L	Q	P	Y	Q	V	M	W	L	E
Template Known Secondary structure				T	T	T	T	T	T	S		S					T	T	T						S	S	S						T	T
Template Predicted Secondary structure
Template SS confidence
		588	.	590	.	.	.	.	.	.	.	.	.	600	.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.