Email l.a.kelley@imperial.ac.uk Description C6Y4C7 Date Sun Jul 8 11:45:09 BST 2012 Unique Job ID 8c23cf585e88a7ea

Summary

Top model

Image coloured by rainbow N → C terminus Close Jmol

Model (left) based on template d1y76a1 Top template information Fold:L27 domain Superfamily:L27 domain Family:L27 domain Confidence and coverage Confidence: 49.5% Coverage: 46% 32 residues ( 46% of your sequence) have been modelled with 49.5% confidence by the single highest scoring template. You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library. Please note: You must be registered and logged in to use Phyrealarm. 3D viewing Interactive 3D view in Jmol

Sequence analysis

Secondary structure and disorder prediction

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1y76 chain A domain 1 Region: 35 - 66 Aligned: 32 Modelled: 32 Confidence: 49.5% Identity: 9% Fold: L27 domain Superfamily: L27 domain Family: L27 domain Phyre2 PDB 3dwl chain I Region: 30 - 56 Aligned: 27 Modelled: 27 Confidence: 38.2% Identity: 33% PDB header:structural protein Chain: I: PDB Molecule:actin-related protein 2/3 complex subunit 2; PDBTitle: crystal structure of fission yeast arp2/3 complex lacking the arp22 subunit Phyre2 PDB 1k8k chain D domain 2 Region: 30 - 52 Aligned: 23 Modelled: 23 Confidence: 33.1% Identity: 17% Fold: Secretion chaperone-like Superfamily: Arp2/3 complex subunits Family: Arp2/3 complex subunits Phyre2 PDB 2p9l chain D Region: 30 - 52 Aligned: 23 Modelled: 23 Confidence: 26.7% Identity: 17% PDB header:structural protein Chain: D: PDB Molecule:actin-related protein 2/3 complex subunit 2; PDBTitle: crystal structure of bovine arp2/3 complex Phyre2 PDB 1vf6 chain B Region: 38 - 66 Aligned: 29 Modelled: 29 Confidence: 25.2% Identity: 10% PDB header:protein binding/protein transport Chain: B: PDB Molecule:pals1-associated tight junction protein; PDBTitle: 2.1 angstrom crystal structure of the pals-1-l27n and patj2 l27 heterodimer complex Phyre2 PDB 2e74 chain G domain 1 Region: 15 - 27 Aligned: 13 Modelled: 13 Confidence: 19.9% Identity: 46% Fold: Single transmembrane helix Superfamily: PetG subunit of the cytochrome b6f complex Family: PetG subunit of the cytochrome b6f complex Phyre2 PDB 1vf5 chain G Region: 15 - 27 Aligned: 13 Modelled: 13 Confidence: 18.4% Identity: 46% Fold: Single transmembrane helix Superfamily: PetG subunit of the cytochrome b6f complex Family: PetG subunit of the cytochrome b6f complex Phyre2 PDB 1vf5 chain G Region: 15 - 27 Aligned: 13 Modelled: 13 Confidence: 18.4% Identity: 46% PDB header:photosynthesis Chain: G: PDB Molecule:protein pet g; PDBTitle: crystal structure of cytochrome b6f complex from m.laminosus Phyre2 PDB 1cf7 chain A Region: 3 - 10 Aligned: 8 Modelled: 8 Confidence: 13.9% Identity: 63% Fold: DNA/RNA-binding 3-helical bundle Superfamily: "Winged helix" DNA-binding domain Family: Cell cycle transcription factor e2f-dp Phyre2 PDB 2l1l chain A Region: 57 - 69 Aligned: 13 Modelled: 13 Confidence: 10.8% Identity: 54% PDB header:nuclear protein Chain: A: PDB Molecule:camp-dependent protein kinase inhibitor alpha; PDBTitle: nmr solution structure of the phi0 pki nes peptide in complex with2 crm1-rangtp Phyre2 PDB 2l9u chain A Region: 1 - 36 Aligned: 36 Modelled: 36 Confidence: 9.4% Identity: 25% PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-3; PDBTitle: spatial structure of dimeric erbb3 transmembrane domain Phyre2 PDB 2rmz chain A Region: 17 - 30 Aligned: 14 Modelled: 14 Confidence: 9.2% Identity: 21% PDB header:cell adhesion Chain: A: PDB Molecule:integrin beta-3; PDBTitle: bicelle-embedded integrin beta3 transmembrane segment Phyre2 PDB 2e74 chain D domain 2 Region: 8 - 28 Aligned: 21 Modelled: 21 Confidence: 8.1% Identity: 38% Fold: Single transmembrane helix Superfamily: ISP transmembrane anchor Family: ISP transmembrane anchor Phyre2 PDB 1vf6 chain A Region: 41 - 66 Aligned: 26 Modelled: 26 Confidence: 7.4% Identity: 12% Fold: L27 domain Superfamily: L27 domain Family: L27 domain Phyre2 PDB 1lbj chain A Region: 44 - 55 Aligned: 12 Modelled: 12 Confidence: 7.1% Identity: 42% PDB header:hormone/growth factor Chain: A: PDB Molecule:motilin; PDBTitle: nmr solution structure of motilin in phospholipid bicellar2 solution Phyre2 PDB 1jdh chain B Region: 45 - 66 Aligned: 22 Modelled: 22 Confidence: 7.0% Identity: 32% PDB header:transcription Chain: B: PDB Molecule:htcf-4; PDBTitle: crystal structure of beta-catenin and htcf-4 Phyre2 PDB 2klu chain A Region: 12 - 56 Aligned: 45 Modelled: 45 Confidence: 6.8% Identity: 24% PDB header:immune system, membrane protein Chain: A: PDB Molecule:t-cell surface glycoprotein cd4; PDBTitle: nmr structure of the transmembrane and cytoplasmic domains2 of human cd4 Phyre2 PDB 2p92 chain A domain 1 Region: 14 - 25 Aligned: 12 Modelled: 12 Confidence: 6.3% Identity: 33% Fold: eIF1-like Superfamily: TM1457-like Family: TM1457-like Phyre2 PDB 2knc chain B Region: 17 - 56 Aligned: 40 Modelled: 40 Confidence: 6.0% Identity: 20% PDB header:cell adhesion Chain: B: PDB Molecule:integrin beta-3; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 2g0i chain A domain 1 Region: 14 - 25 Aligned: 12 Modelled: 12 Confidence: 5.9% Identity: 33% Fold: eIF1-like Superfamily: TM1457-like Family: TM1457-like Phyre2 1 d1y76a1 2 c3dwlI_ 3 d1k8kd2 4 c2p9lD_ 5 c1vf6B_ 6 d2e74g1 7 d1vf5g_ 8 c1vf5G_ 9 d1cf7a_ 10 c2l1lA_ 11 c2l9uA_ 12 c2rmzA_ 13 d2e74d2 14 d1vf6a_ 15 c1lbjA_ 16 c1jdhB_ 17 c2kluA_ 18 d2p92a1 19 c2kncB_ 20 d2g0ia1 21 22

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0