Email l.a.kelley@imperial.ac.uk Description C6Y4A4 Date Sun Jul 8 11:45:05 BST 2012 Unique Job ID 0cafb4733d507466

Summary

Top model

Image coloured by rainbow N → C terminus Close Jmol

Model (left) based on template c3fqgA_ Top template information PDB header:protein binding Chain: A: PDB Molecule:protein din1; PDBTitle: crystal structure of the s. pombe rai1 Confidence and coverage Confidence: 17.6% Coverage: 12% 13 residues ( 12% of your sequence) have been modelled with 17.6% confidence by the single highest scoring template. You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library. Please note: You must be registered and logged in to use Phyrealarm. 3D viewing Interactive 3D view in Jmol

Sequence analysis

Secondary structure and disorder prediction

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3fqg chain A Region: 84 - 96 Aligned: 13 Modelled: 13 Confidence: 17.6% Identity: 23% PDB header:protein binding Chain: A: PDB Molecule:protein din1; PDBTitle: crystal structure of the s. pombe rai1 Phyre2 PDB 1z65 chain A Region: 53 - 66 Aligned: 14 Modelled: 14 Confidence: 16.8% Identity: 29% PDB header:unknown function Chain: A: PDB Molecule:prion-like protein doppel; PDBTitle: mouse doppel 1-30 peptide Phyre2 PDB 1oed chain E Region: 49 - 107 Aligned: 58 Modelled: 59 Confidence: 16.2% Identity: 12% Fold: Neurotransmitter-gated ion-channel transmembrane pore Superfamily: Neurotransmitter-gated ion-channel transmembrane pore Family: Neurotransmitter-gated ion-channel transmembrane pore Phyre2 PDB 1sdb chain B Region: 62 - 77 Aligned: 14 Modelled: 16 Confidence: 16.2% Identity: 36% PDB header:hormone Chain: B: PDB Molecule:desb1-2 despentapeptide (b26-b30) insulin; PDBTitle: porcine desb1-2 despentapeptide(b26-b30) insulin Phyre2 PDB 1dei chain B Region: 62 - 77 Aligned: 14 Modelled: 16 Confidence: 15.9% Identity: 36% PDB header:hormone Chain: B: PDB Molecule:insulin; PDBTitle: desheptapeptide (b24-b30) insulin Phyre2 PDB 2axt chain I domain 1 Region: 85 - 97 Aligned: 13 Modelled: 13 Confidence: 14.2% Identity: 31% Fold: Single transmembrane helix Superfamily: Photosystem II reaction center protein I, PsbI Family: PsbI-like Phyre2 PDB 1his chain B Region: 62 - 77 Aligned: 14 Modelled: 16 Confidence: 13.6% Identity: 36% PDB header:hormone Chain: B: PDB Molecule:insulin; PDBTitle: structure and dynamics of des-pentapeptide-insulin in2 solution: the molten-globule hypothesis. Phyre2 PDB 3fqi chain A Region: 84 - 96 Aligned: 13 Modelled: 13 Confidence: 13.3% Identity: 38% PDB header:protein binding Chain: A: PDB Molecule:protein dom3z; PDBTitle: crystal structure of the mouse dom3z Phyre2 PDB 2rn5 chain B Region: 62 - 77 Aligned: 14 Modelled: 16 Confidence: 10.7% Identity: 36% PDB header:hormone Chain: B: PDB Molecule:insulin; PDBTitle: humal insulin mutant b31lys-b32arg Phyre2 PDB 3pcq chain M Region: 55 - 72 Aligned: 18 Modelled: 18 Confidence: 10.2% Identity: 28% PDB header:photosynthesis Chain: M: PDB Molecule:photosystem i reaction center subunit xii; PDBTitle: femtosecond x-ray protein nanocrystallography Phyre2 PDB 3cx5 chain E domain 2 Region: 68 - 92 Aligned: 25 Modelled: 25 Confidence: 8.6% Identity: 32% Fold: Single transmembrane helix Superfamily: ISP transmembrane anchor Family: ISP transmembrane anchor Phyre2 PDB 1kzy chain C domain 2 Region: 83 - 94 Aligned: 12 Modelled: 12 Confidence: 7.7% Identity: 33% Fold: BRCT domain Superfamily: BRCT domain Family: 53BP1 Phyre2 1 c3fqgA_ 2 c1z65A_ 3 d1oede_ 4 c1sdbB_ 5 c1deiB_ 6 d2axti1 7 c1hisB_ 8 c3fqiA_ 9 c2rn5B_ 10 c3pcqM_ 11 d3cx5e2 12 d1kzyc2

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0