BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (95 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q02885 Uncharacterized protein yubO n=25 Tax=Enterobact...   200   1e-50
UniRef50_C8T078 Putative uncharacterized protein n=1 Tax=Klebsie...   120   1e-26

>UniRef50_Q02885 Uncharacterized protein yubO n=25 Tax=Enterobacteriaceae
          RepID=YUBO_ECOLI
          Length = 95

 Score =  200 bits (509), Expect = 1e-50,   Method: Compositional matrix adjust.
 Identities = 95/95 (100%), Positives = 95/95 (100%)

Query: 1  MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRKSHPEKMAFSYSYALSR 60
          MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRKSHPEKMAFSYSYALSR
Sbjct: 1  MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRKSHPEKMAFSYSYALSR 60

Query: 61 GVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC 95
          GVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC
Sbjct: 61 GVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC 95


>UniRef50_C8T078 Putative uncharacterized protein n=1 Tax=Klebsiella pneumoniae
           subsp. rhinoscleromatis ATCC 13884 RepID=C8T078_KLEPR
          Length = 104

 Score =  120 bits (302), Expect = 1e-26,   Method: Compositional matrix adjust.
 Identities = 60/104 (57%), Positives = 72/104 (69%), Gaps = 13/104 (12%)

Query: 1   MSTRNIHVNTA-------------SYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRKSHP 47
           M TRN++V TA             SY +LVA   KN  EEWDV+EFSSL  LKK+R+  P
Sbjct: 1   MHTRNVNVKTAAQESSRKMGGKPVSYRILVADMPKNAEEEWDVMEFSSLAVLKKFRRRCP 60

Query: 48  EKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDI 91
           EKM+F Y YALS GVD QF+HIN+ EADHFKQF+R I+ AG+DI
Sbjct: 61  EKMSFIYGYALSSGVDKQFQHINVIEADHFKQFVRLIEHAGIDI 104


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q02885 Uncharacterized protein yubO n=25 Tax=Enterobact...   165   3e-40
UniRef50_C8T078 Putative uncharacterized protein n=1 Tax=Klebsie...   154   9e-37

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_Q02885 Uncharacterized protein yubO n=25 Tax=Enterobacteriaceae
          RepID=YUBO_ECOLI
          Length = 95

 Score =  165 bits (419), Expect = 3e-40,   Method: Composition-based stats.
 Identities = 95/95 (100%), Positives = 95/95 (100%)

Query: 1  MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRKSHPEKMAFSYSYALSR 60
          MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRKSHPEKMAFSYSYALSR
Sbjct: 1  MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRKSHPEKMAFSYSYALSR 60

Query: 61 GVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC 95
          GVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC
Sbjct: 61 GVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC 95


>UniRef50_C8T078 Putative uncharacterized protein n=1 Tax=Klebsiella pneumoniae
           subsp. rhinoscleromatis ATCC 13884 RepID=C8T078_KLEPR
          Length = 104

 Score =  154 bits (389), Expect = 9e-37,   Method: Composition-based stats.
 Identities = 60/104 (57%), Positives = 72/104 (69%), Gaps = 13/104 (12%)

Query: 1   MSTRNIHVNTA-------------SYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRKSHP 47
           M TRN++V TA             SY +LVA   KN  EEWDV+EFSSL  LKK+R+  P
Sbjct: 1   MHTRNVNVKTAAQESSRKMGGKPVSYRILVADMPKNAEEEWDVMEFSSLAVLKKFRRRCP 60

Query: 48  EKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDI 91
           EKM+F Y YALS GVD QF+HIN+ EADHFKQF+R I+ AG+DI
Sbjct: 61  EKMSFIYGYALSSGVDKQFQHINVIEADHFKQFVRLIEHAGIDI 104


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.322    0.139    0.404 

Lambda     K      H
   0.267   0.0434    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 369,180,627
Number of Sequences: 3077464
Number of extensions: 12983606
Number of successful extensions: 31359
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 31354
Number of HSP's gapped (non-prelim): 4
length of query: 95
length of database: 1,040,396,356
effective HSP length: 64
effective length of query: 31
effective length of database: 843,438,660
effective search space: 26146598460
effective search space used: 26146598460
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 87 (38.0 bits)