BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (73 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P64523 Uncharacterized protein yeeT n=144 Tax=Enterobac...   156   2e-37
UniRef50_B8FNH6 Putative uncharacterized protein n=1 Tax=Desulfa...    54   2e-06

>UniRef50_P64523 Uncharacterized protein yeeT n=144 Tax=Enterobacteriaceae
          RepID=YEET_ECO57
          Length = 73

 Score =  156 bits (394), Expect = 2e-37,   Method: Compositional matrix adjust.
 Identities = 73/73 (100%), Positives = 73/73 (100%)

Query: 1  MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIPGVLAVFAERRKDS 60
          MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIPGVLAVFAERRKDS
Sbjct: 1  MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIPGVLAVFAERRKDS 60

Query: 61 FGPYVRLMSVTLN 73
          FGPYVRLMSVTLN
Sbjct: 61 FGPYVRLMSVTLN 73


>UniRef50_B8FNH6 Putative uncharacterized protein n=1 Tax=Desulfatibacillum
          alkenivorans AK-01 RepID=B8FNH6_DESAA
          Length = 81

 Score = 53.5 bits (127), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 29/79 (36%), Positives = 45/79 (56%), Gaps = 15/79 (18%)

Query: 1  MKIITRGEAMRIHQQH----------PTSRLFPFCTGKYRWHGSAEAYTGREVQDI-PGV 49
          MKI+T+ +A+ +  +           PT     + +GKY+W G A  YTG ++  + P +
Sbjct: 1  MKIVTKKDAVELASKTILDGKNRAKTPTK----YDSGKYKWTGKASDYTGVDIDFVWPEL 56

Query: 50 LAVFAERRKDSFGPYVRLM 68
          LAV+ ERR D+ GPY +LM
Sbjct: 57 LAVYVERRSDANGPYAQLM 75


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P64523 Uncharacterized protein yeeT n=144 Tax=Enterobac...   141   1e-32
UniRef50_B8FNH6 Putative uncharacterized protein n=1 Tax=Desulfa...    99   4e-20

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P64523 Uncharacterized protein yeeT n=144 Tax=Enterobacteriaceae
          RepID=YEET_ECO57
          Length = 73

 Score =  141 bits (354), Expect = 1e-32,   Method: Composition-based stats.
 Identities = 73/73 (100%), Positives = 73/73 (100%)

Query: 1  MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIPGVLAVFAERRKDS 60
          MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIPGVLAVFAERRKDS
Sbjct: 1  MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIPGVLAVFAERRKDS 60

Query: 61 FGPYVRLMSVTLN 73
          FGPYVRLMSVTLN
Sbjct: 61 FGPYVRLMSVTLN 73


>UniRef50_B8FNH6 Putative uncharacterized protein n=1 Tax=Desulfatibacillum
          alkenivorans AK-01 RepID=B8FNH6_DESAA
          Length = 81

 Score = 99.0 bits (245), Expect = 4e-20,   Method: Composition-based stats.
 Identities = 27/75 (36%), Positives = 43/75 (57%), Gaps = 7/75 (9%)

Query: 1  MKIITRGEAMRIHQQH------PTSRLFPFCTGKYRWHGSAEAYTGREVQDI-PGVLAVF 53
          MKI+T+ +A+ +  +              + +GKY+W G A  YTG ++  + P +LAV+
Sbjct: 1  MKIVTKKDAVELASKTILDGKNRAKTPTKYDSGKYKWTGKASDYTGVDIDFVWPELLAVY 60

Query: 54 AERRKDSFGPYVRLM 68
           ERR D+ GPY +LM
Sbjct: 61 VERRSDANGPYAQLM 75


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.311    0.131    0.376 

Lambda     K      H
   0.267   0.0386    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 258,023,748
Number of Sequences: 3077464
Number of extensions: 7527135
Number of successful extensions: 20024
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 20018
Number of HSP's gapped (non-prelim): 4
length of query: 73
length of database: 1,040,396,356
effective HSP length: 44
effective length of query: 29
effective length of database: 904,987,940
effective search space: 26244650260
effective search space used: 26244650260
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (20.9 bits)
S2: 87 (38.2 bits)